Production of nicotinamide adenine dinucleotide

Abstract

Abstract PURPOSE:To obtain nicotinamide adenine dinucleotide (NAD) from a culture mixture efficiently, by cultivating yeast belonging to the genus Candida in a medium comprising methanol as a main carbon source. CONSTITUTION:Yeast such as Candida 25A (FERM 8725) or Candida methanolica (ATCC 26175) belonging to the genus Candida is cultivated in a nutritive medium containing about 1-3vol% methanol preferably at pH 4-7 at 25-35 deg.C while aerated stirring. NAD content can be improved when about 0.1-0.5wt% quinolinic acid is added to the medium at any period in the middle of the cultivation. Molds are collected from the prepared culture mixture, ground and NAD is obtained from supernatant liquid of the molds.

Inventors

• 👤 Yoshiki Tani
• 👤 吉樹 谷
• 👤 Akira Takeda
• 👤 明 武田

Bibliographic Details