Production of branched cyclodextrin

Abstract

Abstract PURPOSE:To obtain the titled compound having high solubility in water, in remarkably improved reaction rate and yield, by reacting cyclodextrin with glycose having fluorine atom bonded to the reducing terminal carbon atom in the presence of isoamylase. CONSTITUTION:The objective compound can be produced by (1) reacting (A) cyclodextrin (e.g. alpha-cyclodextrin) with (B) a glycol of formula I having fluorine atom bonded to the reducing terminal carbon atom or a malto-oligosaccharide of formula II (n is integer of 1-3) (e.g., alpha-maltosyl fluoride) in the presence of an isoamylase at 40 deg.C, (2) adding an aqueous solution of sodium hydroxide to the reaction liquid, (3) heating the mixture at 100 deg.C for 30min to effect the neutralization of the product and (4) purifying the product by ion-exchange treatment and gel-filtration.

Inventors

• 👤 Yoshinori Yoshimura
• 👤 佳典 吉村
• 👤 Shigetaka Okada
• 👤 岡田　茂孝
• 👤 Sumio Kitahata
• 👤 北畑　寿美雄

Bibliographic Details