Novel method for quantitative determination of antigen

Abstract

Abstract PURPOSE:To determine indirectly an antigen from the hemolysis of red blood cells by complement fixation reaction by bringing the antigen to be quantitatively determined and antibody into reaction directly on the surface of the red blood cells. CONSTITUTION:The 1st antibody is added to a red blood cell suspension formed by bonding the 2nd antibody for the immune globlin protein of an animal of the same kind as the 1st antibody for the antigen to be quantitatively determined with said 1st antibody and thereafter the antigen and complement are further added thereto to bring the same into reaction and to hemolyze the red blood cells, by which the antigen is indirectly and quantitatively determined from said hemolysis.

Inventors

• 👤 Shosaku Motoda
• 👤 昭策 元田
• 👤 Shigeru Sekine
• 👤 盛 関根
• 👤 Satoru Imai
• 👤 悟 今井

Bibliographic Details