Data processing in chromatography

Abstract

Abstract PURPOSE:To automatically perform analysis with high accuracy, in the comparison of a plurality of chromatograms, by determining the holding times of one or plural main peaks in each chromatogram and performing data processing so as to coincide with the holding time of each chromatogram with each of said determined holding times. CONSTITUTION:When a plurality of chromatograms are compared to investigate correspondence, one or plural main peaks are determined in each chromatogram. Next, the holding time of each chromatogram is converted so that the holding times of these main peaks are substantially coincided between chromatograms to each other. This conversion is performed by multiplying the holding time by coefficient, by adding or subtracting constant with respect to the holding time, a primary linear regression equation, a min./max. method or spline function. Because the peak coming to the reference of holding time conversion may be determined from expericenc even if the condition of the gradient or flow amount of a migration phase is changed, the analysis of each peak can be performed automatically in almost all cases by a computer.

Inventors

• 👤 Kiyoharu Nakatsuka
• 👤 中塚　木代春
• 👤 Masahide Ikeou
• 👤 池応　真英

Bibliographic Details