Column for chromatography

Abstract

Abstract PURPOSE:To increase the limiting flow rate for supplying a fluid sample, and to make quick analysis by using hydroxy-apatite [Ca10(PO4)6(OH)2] having a specific grain size range as a stationary material 3 of column chromatography. CONSTITUTION:A stationary material 3 is packed in a column 1 of chromatography and a fluid sample is supplied thereto through a sample port 5. The sample is separated by the column 1 and is discharged through an outflow port 6. Hydroxy-apatite crystal particles are used as the stationary material. The grain size thereof is made to contain small grain sizes below <=10mum stokes equiv. diameter or even if such small particles are contained therein, the mixing rate thereof is made <=1%. The preferable grain size is in the stokes grain size range of about 20-100mum. The limiting flow rate for supply is therefore increased if the hydroxy-apatite having such grain size is used and since the sepn. accuracy is good, a molecular sepn. of DAN.RAN, etc, and sepn. of the high molecular components in blood are efficiently effected.

Inventors

• 👤 Tsutomu Kawasaki
• 👤 力 川崎

Bibliographic Details