Reagent for immunological analysis

Abstract

Abstract PURPOSE:To widen the application range of a material to be detected by constituting a reagent in such a way that the 2nd antibody is deposited on a microcapsule. CONSTITUTION:This reagent 10 for immunological analysis consists of a microcapsule 11 consisting at least partly of a liquid film, the 2nd antibody 12, which is fixed onto the capsule 11, for the 1st antibody and a labeled material 13 sealed in said capsule 11. The reagent 10, a substantial amt. of the 1st antibody 14 and an antigen 15 which is the material to be detected are first mixed in a suitable buffer soln. to induce an antigen-antiboty reaction and to obtain an antigen-1st antibody-2nd antibody sensitized microcapsule 16. When a substantial amt. of complement 17 diluted with a suitable buffer is added thereto, the capsule 11 is broken by the effect of the complement 17 according to the quantity of the antigen 15, i.e., the antigen-(1st) antiboty reacting weight and the material 13 is released from the inside of the capsule 11. The quantity of the material 15 in the sample is measured by the analyzing method meeting the material 13.

Inventors

• 👤 Yoshio Ishimori
• 👤 石森　義雄
• 👤 Masako Notsuke
• 👤 野附　正子
• 👤 Masao Koyama
• 👤 小山　昌夫

Bibliographic Details