Enzyme electrode

Abstract

Abstract PURPOSE:To check an impeding material and to prevent deaceleration of responding rate and reduction in sensitivity, by a method wherein an acetyl-cellulose layer is formed in an aperture in a porous film or on one film surface, an enzyme is fixed to the one film surface, and H2O2 detecting electrode is formed on the other film surface. CONSTITUTION:One surface side of a porous film 1 comprising polycarbonate having no swelling property against water is immersed in an aceton solution consisting of acetylcellulose and is dried. The operation is repeated several times to form an acetylcellulose layer 2 in an aperture in the film 1 or on one side surface. A H2O2 detecting electrode 4 is formed on other side surface of the film 1 by spattering Pt and the like. A water thick solution of a glucol oxidase 3 is spread on the film 1 surface located opposite to an electrode 4, and is dried to fix said enzyme 3 in glutaraldehyde vapor to obtain an enzyme electrode 8. The electrode 8 is held to a cylindrical body 11 with a cap 10 through the medium of a packing 9. This causes a reliable measurement of concentration of a substrate, i.e., glucose, in a sample without being affected by uric acid, ascorbic acid and without reduction in a responding rate and sensitivity, and enables the enzyme electrode to be repeatedly used.

Inventors

• 👤 Shiro Nankai
• 👤 史朗 南海
• 👤 Akihiro Imai
• 👤 章博 今井
• 👤 Takashi Iijima
• 👤 孝志 飯島

Bibliographic Details