Reagent for quantitative determination of hydrogen peroxide

Abstract

Abstract PURPOSE:To obtain a reagent easy to prepare a uniform aqueous solution and having no influence on the activity of enzyme and also, having good reproducibility, by using specific triaryl imidazole derivative etc. having a sulfonic acid radical or carboxyl group. CONSTITUTION:A compound expressed by the general formula in the figure (wherein R<1> is -H, -OCH3-CH3, R<2> is -CH3,-C2H5, R<3> is -(CH2)n-SO3H or -(CH2)n- COOH and n=1-3) or its salt, is incorporated as a color forming agent. For example, a reagent is constituted by desired reagent components participating in a series of reaction which produce hydrogen peroxide finally by acting to a substance and peroxidase or the substance acting like peroxidase and also, said compound. Said compound is used effectively for the determination of trace components because it is easily soluble in water and the maximum absorption wavelength of coloring matter produced by reacting with hydrogen peroxide is 610mm. and said compound is not affected by the substance having an absorption band in a short wavelength e.g. bilirubin, hemoglobin etc. at measurement and moreover, it has a high molecular absorption coefficient.

Inventors

• 👤 Eigou Yuki
• 👤 英剛 由岐
• 👤 ▲さい▼藤　仁俊
• 👤 Hitotoshi Saitou
• 👤 Kazuyoshi Nishidate
• 👤 西館　和由
• 👤 Norimasa Takizawa
• 👤 滝沢　徳正

Bibliographic Details