Measuring method of amylase activity

Abstract

Abstract PURPOSE: To measure the titled substance accurately, by adding cyclodextrin to a buffer solution containing a maltooligosaccharide having phenyl halide groups linked to the reducing terminal groups as a substrate. CONSTITUTION: In dissolving a maltooligosaccharide having phenyl halide groups linked to the reducing terminal groups in a buffer solution, cyclodextrin is added thereto. The amount of the cyclodextrin is about 0.5W5wt% based on the buffer solution. A sample is then reacted with the resultant substrate solution, and then α-glucosidase and β-glucosidase are reacted therewith to measure the liberated halogenophenol. Thus, the amylase activity in the sample can be measured accurately. COPYRIGHT: (C)1982,JPO&Japio

Inventors

• 👤 Takaaki Kanda
• 👤 Yoshitaka Nakagiri
• 👤 Shigetaka Okada

Bibliographic Details