Enzyme electrode

Abstract

Abstract PURPOSE:To obtain the enzyme electrode having quick response by providing a platinum layer on a conductive substrate as an electrode for detecting hydrogen and directly fixing enzyme on said electrode. CONSTITUTION:The pellet shaped conductive substrate 3 is formed by compressing the mixture of 10pts.wt. of fluororesin powder as a binding agent and 90pts.wt. of graphite. Then the platinum layer 2 is provided on the surface of said conductive substrate 3 by the electrolysis of aqueous rolution of chloroplatinic acid and the electrode for detecting hydrogen peroxide is obtained. On said electrode, is applied aqueous solution of glucose oxidase. After it has been dried, the device is reacted at 25 deg.C for about one hour in the vapor of alutaric aldehyde, and bridging and fixing are made. Thereafter, the device is well washed, the material not reacted is removed, and an enzyme fixed layer 1 is formed. The enzyme electrode obtained in this way indicates quick response, and its characteristics will not change for a long time even though it is repeatedly measured and washed.

Inventors

• 👤 Shiro Nankai
• 👤 Kenichi Nakamura
• 👤 Takashi Iijima

Bibliographic Details