Enzyme sensor and production thereof

Abstract

Abstract PURPOSE:To easily, rapidly and accurately measure the substrate concn. of glucose, etc., by measuring an enzyme reaction from the change in the electron transfer reaction by an electron transfer medium effected between the enzyme in a monomolecular film and a conductive base body. CONSTITUTION:An LB (Langmuir-Blodgett) film 3 by an LB technique is formed as the monomolecular film immobilized with the electron transfer medium together with the enzyme on the surface of the conductive base body 4 produced by depositing an iridium oxide film 2a on a glass plate 1 and further a thin film 2b of platinum thereon. The electron transfer is effected in such a manner between the enzyme and the conductive base body so as to cause the indirect oxidation reduction of the enzyme. The enzyme reaction is directly electrochemically detected and the substrate concn. is measured by the increased current value on the conductive base body. The response of the enzyme reaction is accurately measured in this way even if the substrate concn. is low. The response characteristic is thus improved. The construction is simplified and the measurement is facilitated. Further, the time for the measurement is shortened.

Inventors

• 👤 Teruaki Katsube
• 👤 勝部　昭明
• 👤 Takeshi Shimomura
• 👤 猛 下村
• 👤 Hideichiro Yamaguchi
• 👤 秀一郎 山口

Bibliographic Details