Dna repair and mutation inducing method

Abstract

Abstract PURPOSE: To induce a site-specific mutation by simultaneously culturing a cleaved linear target DNA and an oligonucleotide having a nucleotide sequence complementary to both the side chains of the cleaved site in a proper host cell. CONSTITUTION: This method for inducing the repair and mutation of a DNA comprises cleaving the DNA at a site near to a DNA subregion to be deleted, mixing the cleaved DNA with an oligonucleotide having a nucleotide sequence complementary to both the side chains of the cleaved site and corresponding to a desired DNA sequence after the deletion, inserting the modified mixture into a cell and subsequently culturing the cell. When plasmid pWM300 is cleaved with SmaI enzyme and subsequently mutated with an oligonucleotide of the Figure, plasmid pWM301 is obtained. COPYRIGHT: (C)1990,JPO

Inventors

• 👤 Wlodzimierz Mandecki
• 👤 ロッツィミールツ・マンデッキ
• 👤 Mark Allan Hayden
• 👤 マーク・アラン・ハイデン

Bibliographic Details