Enzyme preparations

Abstract

Abstract 1385319 Cholesterol oxidase ex mycobacterium rhodocrons group NATIONAL RESEARCH DEVELOPMENT CORP 18 Sept 1972 [22 Sept 1971 19 June 1972] 44095/71 and 28650/72 Heading C3H An enzyme preparation derived from a cholesterol oxidase producing microorganism of the Mycobacterium rhodocrons group and especially from Nocardia species NCIB 10554 and 10555 and having a cholesterol oxidase specific activity of at least 1 unit per 5mg. of protein nitrogen may be obtained by growing said cholesterol oxidase producing microorganism and recovering therefrom an enzyme preparation as defined. The enzyme may be recovered by harvesting the cells and extracting with a surface active agent (iso-octyl phenoxy polyethoxy ethanol) and may be purified by ion exchange or affinity chromatography.

Inventors

No inventors recorded.

Bibliographic Details