MONOCATENARY OLIGONUCLEOTIDE COMPOSITIONS AND PROCESS FOR THEIR PREPARATION

Abstract

Abstract Translated from French L'INVENTION CONCERNE NOTAMMENT UN PROCEDE POUR LA SYNTHESE D'OLIGONUCLEOTIDES QUI CONTIENNENT UN OU DES GROUPE(S) AMINO ALIPHATIQUE(S) LIBRE(S). LE PROCEDE DE SYNTHESE EST GENERAL, PERMETTANT DE FIXER DES GROUPES AMINO SUR DES OLIGONUCLEOTIDES DE TOUTE COMPOSITION OU LONGUEUR OBTENABLE PAR DES PROCEDES USUELS DE SYNTHESE D'ADN. APPLICATION A LA BIOLOGIE MOLECULAIRE ET NOTAMMENT AU SEQUENCAGE AUTOMATISE D'ADN, A LA DETECTION DE TROUBLES GENETIQUES PAR HYBRIDATION D'ADN ET A L'UTILISATION DE LA FLUORESCENCE POUR LA DETECTION D'UNE HYBRIDATION. THE INVENTION CONCERNS IN PARTICULAR A PROCESS FOR THE SYNTHESIS OF OLIGONUCLEOTIDES WHICH CONTAIN ONE OR MORE FREE ALIPHATIC AMINO GROUP (S). THE SYNTHESIS PROCESS IS GENERAL, ALLOWING TO FIX AMINO GROUPS ON OLIGONUCLEOTIDES OF ANY COMPOSITION OR LENGTH OBTAINABLE BY USUAL DNA SYNTHESIS METHODS. APPLICATION TO MOLECULAR BIOLOGY AND IN PARTICULAR TO AUTOMATED DNA SEQUENCING, TO THE DETECTION OF GENETIC DISORDERS BY DNA HYBRIDATION AND TO THE USE OF FLUORESCENCE FOR THE DETECTION OF A HYBRIDATION.

Inventors

• 👤 Lloyd M Smith

Bibliographic Details