EP0964060A2
Expired / Lapsed
EP
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Method for quantitating a substrate and measurement device used therefor
Assignee: Panasonic Holdings Corp
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Filing: 1999-06-08
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Granted: —
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Expires: 2019-06-08
Abstract
Abstract The present invention provides a measurement device facilitating accurate quantitation of a substrate from a trace amount of sample and a highly reliable quantitating method of a substrate. The method uses an analysis element comprising a pair of electrodes for electrochemically quantitating reaction between a substrate in a sample and an oxidoreductase. One of the electrodes of the analysis element is a membrane formed on an inner wall of a cylindrical hollow space having an opening and the other is a needle. The needle electrode is projected temporarily external to the hollow space to puncture a subject and the resultant sample is collected from the subject.
Inventors
- 👤 Shin Ikeda
- 👤 Motokazu Watanabe
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 20:56
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/145; A61B5/1468; A61B5/1486; A61B5/15; A61B5/150007; A61B5/150015; A61B5/150022; A61B5/150374; A61B5/150381; A61B5/150412; A61B5/150435; A61B5/151; A61B5/15101; A61B5/15103; A61B5/15107; A61B5/15113; A61B5/15115; A61B5/15117; A61B5/15126; A61B5/1513; A61B5/15142; A61B5/15146; A61B5/15148; A61B5/15149; A61B5/15151; A61B5/15157; A61B5/15165; A61B5/15171; A61B5/68; A61B5/6846; A61B5/6847; A61B5/6848; A61B5/14532; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
150 words
A method for quantitating a substrate
comprising the steps of: preparing an analysis element comprising a needle
electrode, a membrane electrode and a cylinder having a
hollow space, said hollow space being open at at least one end
thereof, wherein said needle electrode is accommodated in
said hollow space and said membrane electrode is formed on
an inner wall of said hollow space; pressing an opening of said cylinder against a
subject to be examined; projecting a tip of said needle electrode external
to said opening of said cylinder temporarily to puncture
said subject and collecting a sample from said subject; introducing the collected sample between said
membrane electrode and said needle electrode in said
cylinder; reacting a substrate contained in said sample with
an oxidoreductase; and applying a voltage across said membrane electrode
and said needle electrode and measuring resultant current in
said membrane electrode or said needle electrode.
Claim 2
Dependent Claim
83 words
A measurement device of a substrate for
electrochemically quantitating a concentration of a
substrate contained in a sample based on reaction between
said substrate and an oxidoreductase, said device
comprising: an element having a cylindrical hollow space, said
space being open at at least one end thereof; a membrane electrode formed on an inner wall of
said hollow space; a needle electrode accommodated in said hollow
space; and means for projecting a tip of said needle
electrode external to said hollow space temporarily.
Claim 3
Dependent Claim
29 words
The measurement device of a substrate in
accordance with claim 2, wherein said needle member is
formed with an electrically insulating layer on a portion of
the surface thereof.
Claim 4
Dependent Claim
32 words
The measurement device of a substrate in
accordance with claim 2, wherein said needle member is
formed with a reagent layer containing an electron acceptor
and an oxidoreductase on the surface thereof.
Claim 5
Dependent Claim
34 words
The measurement device of a substrate in
accordance with claim 2, wherein said hollow space is
further formed with a reagent layer containing an electron
acceptor and an oxidoreductase on an inner wall thereof.
Claim 6
Dependent Claim
31 words
The measurement device of a substrate in
accordance with claim 2 further comprising a unit for fixing
the needle electrode at the predetermined position after the
projection of said needle electrode.
References Cited (Backward Citations)
No citation records indexed.
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