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EP0901018A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Method for quantitative measurement of a substrate

Assignee: Panasonic Holdings Corp
•
Filing: 1998-07-24
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Granted: —
•
Expires: 2018-07-24
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Abstract

Abstract The invention provides a method for quantitative measurement of a substrate with high accuracy by electrochemically oxidizing an electron mediator which has been reduced by enzyme reaction thereby determining the substrate concentration based on a current flowing during electrochemical oxidation from which adverse effects of an easy-to-oxidize substance on the oxidation process have been minimized. The quantitating method in accordance with the present invention comprises a first step for causing a substrate contained in a sample to react with a specific oxidoreductase to the substrate in the presence of an electron mediator in oxidized state, and a second step for electrochemically reducing the electron mediator in oxidized state which remains non-reduced by the enzyme reaction in the first step, thereby obtaining a current flowing during electrochemical reduction.

Inventors

  • 👤 Shin Ikeda
  • 👤 Toshihiko Yoshioka
  • 👤 Shiro Nankai

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-28 16:24
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; C12Q1/005; C12Q1/006

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 70 words
A method for quantitative measurement of a substrate comprising: a first step for causing a substrate contained in a sample to react with a specific oxidoreductase to said substrate in the presence of an electron mediator in oxidized state, and a second step for electrochemically reducing said electron mediator in oxidized state which remains non-reduced by enzyme reaction in said first step, thereby obtaining a current flowing during electrochemical reduction.
Claim 2
Dependent Claim 30 words
The method for quantitative measurement of a substrate in accordance with claim 1, wherein an electron mediator in reduced state further participates in the enzyme reaction in said first step.
Claim 3
Dependent Claim 66 words
The method for quantitative measurement of a substrate in accordance with claim 1, wherein said quantitative measurement is conducted using a biosensor comprising an electrically insulating base plate, an electrode system having at least a working electrode and a counter electrode which are formed on said base plate, and a reaction layer including an oxidoreductase and an electron mediator which are disposed on said electrode system.
Claim 4
Dependent Claim 69 words
The method for quantitative measurement of a substrate in accordance with claim 1, wherein said quantitative measurement is conducted using a biosensor comprising an electrically insulating base plate, an electrode system having at least a working electrode, a counter electrode and a reference electrode which are formed on said base plate, and a reaction layer including an oxidoreductase and an electron mediator which are disposed on said electrode system.
Claim 5
Dependent Claim 22 words
The method for quantitative measurement of a substrate in accordance with claim 3, wherein said reaction layer further includes a hydrophilic polymer.
Claim 6
Dependent Claim 22 words
The method for quantitative measurement of a substrate in accordance with claim 4, wherein said reaction layer further includes a hydrophilic polymer.
Claim 7
Dependent Claim 30 words
The method for quantitative measurement of a substrate in accordance with claim 3, wherein an electron mediator in reduced state further participates in the enzyme reaction in said first step.
Claim 8
Dependent Claim 30 words
The method for quantitative measurement of a substrate in accordance with claim 4, wherein an electron mediator in reduced state further participates in the enzyme reaction in said first step.

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