EP0800830A2
Expired / Lapsed
EP
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Method of in vivo delivery of functioning foreign genes
Assignee: Vanderbilt University
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Filing: 1990-10-18
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Granted: —
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Expires: 2010-10-18
Abstract
Abstract A method of expressing a foreign gene in cells of a mammalian organ in vivo includes the step of injecting genetic material capable of inducing desired gene products into a mammal. The genetic material is complexed to cationic liposome carriers. The genetic material is lipofected into cells of the mammalian organ. Gene expression and cellular function of the genetic material are activated to produce the desired foreign products in the cells of the organ.
Inventors
- 👤 Kenneth L. Brigham
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 10:09
CPC Classifications
A; A61; A61K; A61K48/00; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1272
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
74 words
A method of expressing a foreign gene in cells of a mammalian organ in vivo , said method including the steps of: injecting a gene expression inducer of desired gene products into a mammal, the inducer being complexed to a cationic liposome carrier; lipofecting the inducer into cells of the mammalian organ; and activating gene expression and cellular function of the inducer to produce the desired foreign gene products in cells of the organ.
Claim 2
Dependent Claim
30 words
A method as set forth in claim 1 wherein said injecting step is further defined as injecting a plasmid containing the inducer and bound to the carrier into the mammal.
Claim 3
Dependent Claim
27 words
A method as set forth in claim 2 wherein said injecting step is further defined as injecting the plasmid bound to the carrier into the mammal intravenously.
Claim 4
Dependent Claim
49 words
A method as set forth in claim 3 wherein said injecting step is further defined as injecting the plasmid bound to the carrier intravenously into the blood stream of the mammal in proximity to the organ for transfection to increase the activity of the gene expression in the organ.
Claim 5
Dependent Claim
32 words
A method as set forth in claim 1 wherein said activating step is further defined as activating transient expression and cellular function by the inducer to transiently produce the desired gene products.
Claim 6
Dependent Claim
16 words
A method as set forth in claim 1 wherein the inducer is a foreign nucleic acid.
Claim 7
Dependent Claim
23 words
A method as set forth in claim 6 further including the step of promoting expression of particular expression vectors of the nucleic acid.
Claim 8
Dependent Claim
69 words
A method as set forth in claim 7 wherein said injecting step is further defined as injecting carriers containing plasmids containing a coding region for human growth hormone intravenously into a mouse, the region being driven by a mouse metallothionein promoter, said activating step being further defined as feeding the mouse a metallothionein promoter, the promoter activating the production of human growth hormone in the lung of the mouse.
Claim 9
Dependent Claim
43 words
A method as set forth in claim 7 wherein said injecting step is further defined as injecting a carrier containing a plasmid containing a prokaryotic gene, chloramphenicol acetyltransferace, into a mouse and activating chloramphenicol acetyltransferace activity in the lung cells of the mouse.
Claim 10
Dependent Claim
23 words
A method as set forth in claim 9 wherein said injecting step is further defined as injecting the carrier containing the plasmid intratracheally.
References Cited (Backward Citations)
No citation records indexed.
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