EP0731178A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method of measuring oligonucleotide decomposition
Assignee: Hamamatsu Photonics KK
•
Filing: 1996-03-08
•
Granted: —
•
Expires: 2016-03-08
Abstract
Abstract The present invention provides an in situ monitoring of the decomposing activity against oligonucleotides target in a biological tissue. A single-chain oligonucleotide target comprises an appropriate number of nucleic acid bases to be examined with an energy donor and an energy acceptor respectively at its 5'- and 3'-terminals. Monitoring of the fluorescence changes of the target after injection into a biological tissue, particularly the fluorescent resonance energy transfer(FRET) phenomena between the energy donor and acceptor, indicates whether the oligonucleotide is not decomposed yet.
Inventors
- 👤 Hisatoshi Uchiyama
- 👤 Masaki Jibu
- 👤 Kenichi Hirano
- 👤 Kazunari Taira
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 20:55
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
59 words
A method of monitoring the cleavage of an oligonucleotide comprising the steps of; a) detecting a resonance energy transfer caused by an energy donor group and an energy acceptor group combined with the oligonucleotide, b) detecting a change in the resonance energy transfer resulting from decomposition of the oligonucleotide between the energy donor group and the energy acceptor group.
Claim 2
Dependent Claim
26 words
A method according to claim 1, wherein the energy donor group and the energy acceptor group are combined with the 5'- and 3'-terminals of the oligonucleotide.
Claim 3
Dependent Claim
17 words
A method according to claim 1 or claim 2, wherein the fluorescence energy transfer (FRET) is detected.
Claim 4
Dependent Claim
23 words
A method according to claim 3, wherein the energy donor group contains a fluorescein fluorochrome and the energy acceptor contains a rhodamine fluorochrome.
Claim 5
Independent Claim
31 words
A method according to any preceding claim, wherein the oligonucleotide between the energy donor group and the energy acceptor group comprises not less than 3 but not more than 20 nucleotides.
Claim 6
Independent Claim
27 words
A method according to any one of claims 1 to 4, wherein the oligonucleotide between the energy donor group and the energy acceptor group comprises 10 nucleotides.
Claim 7
Independent Claim
12 words
A method according to any preceding claim wherein the oligonucleotide is single-stranded.
Claim 8
Independent Claim
67 words
A method of measuring a decomposing activity against a subject oligonucleotide comprising the steps of detecting a resonance energy transfer caused by a set of energy donor group and energy acceptor group combined with said subject oligonucleotide and then detecting a change in said resonance energy transfer resulting from a decomposition reaction of an oligonucleotide chain between said set of energy donor group and energy acceptor group.
Claim 9
Independent Claim
60 words
A method of measuring a decomposing activity against a subject single-chain oligonucleotide comprising the steps of detecting a resonance energy transfer caused by a set of energy donor group and energy acceptor group combined with 5'- and 3'-terminals of said subject oligonucleotide and then detecting a change in said resonance energy transfer resulting from a decomposition reaction of said oligonucleotide.
Claim 10
Independent Claim
71 words
A method of measuring a decomposing activity against a subject oligonucleotide comprising the steps of detecting a fluorescence resonance energy transfer (FRET) caused by a set of energy donor group and energy acceptor group combined with said subject oligonucleotide and then detecting a change in said fluorescence resonance energy transfer (FRET) resulting from a decomposition reaction of an oligonucleotide chain between said set of energy donor group and energy acceptor group.
Claim 11
Independent Claim
64 words
A method of measuring a decomposing activity against a subject single-chain oligonucleotide comprising the steps of detecting a fluorescence resonance energy transfer (FRET) caused by a set of energy donor group and energy acceptor group combined with 5'- and 3'-terminals of said subject oligonucleotide and then detecting a change in said fluorescence resonance energy transfer (FRET) resulting from a decomposition reaction of said oligonucleotide.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.