EP0636879A2
Expired / Lapsed
EP
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Biosensor and method for producing the same
Assignee: Panasonic Holdings Corp
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Filing: 1994-07-21
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Granted: —
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Expires: 2014-07-21
Abstract
Abstract A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.
Inventors
- 👤 Tomohiro Yamamoto
- 👤 Mariko Miyahara
- 👤 Toshihiko Yoshioka
- 👤 Satoko Fujisawa
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 11:26
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
66 words
A biosensor comprising, an electrical insulating base, an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base, and a reaction layer formed on said insulating base in close contact with said electrode system; wherein said reaction layer contains at least a hydrophilic polymer, an enzyme and a buffer, said enzyme being separated from said buffer.
Claim 2
Dependent Claim
43 words
The biosensor in accordance with claim 1, wherein said reaction layer comprises at least two layers, and wherein a first layer is in contact with said electrode system and contains the enzyme and the hydrophilic polymer, and a second layer contains the buffer.
Claim 3
Dependent Claim
17 words
The biosensor in accordance with claim 2, wherein said second layer is composed of an amphipathic lipid.
Claim 4
Dependent Claim
43 words
The biosensor in accordance with claim 1, wherein said reaction layer comprises at least two layers, and wherein a first layer is in contact with said electrode system and contains the buffer and the hydrophilic polymer, and a second layer contains the enzyme.
Claim 5
Dependent Claim
34 words
The biosensor in accordance with claim 4, wherein said second layer is composed of a hydrophilic polymer soluble in an organic solvent that does not dissolve said hydrophilic polymer contained in said first layer.
Claim 6
Dependent Claim
18 words
The biosensor in accordance with claim 2, wherein either one of said two layers contains an electron acceptor.
Claim 7
Dependent Claim
38 words
The biosensor in accordance with claim 1, wherein said reaction layer comprises at least three layers, and a first layer contains the buffer and the hydrophilic polymer, and a second layer contains the enzyme and the hydrophilic polymer.
Claim 8
Dependent Claim
15 words
The biosensor in accordance with claim 7, wherein said second layer contains an electron acceptor.
Claim 9
Dependent Claim
22 words
The biosensor in accordance with claim 7, further comprising a third layer containing a lipid placed outside said first and second layers.
Claim 10
Dependent Claim
27 words
The biosensor in accordance with claim 7, further comprising a third layer consisting essentially of a hydrophilic polymer and placed in close contact with said electrode system.
Claim 11
Dependent Claim
56 words
The biosensor in accordance with claim 7, wherein said first layer is in close contact with said second layer, wherein said hydrophilic polymers are different from each other, and wherein the hydrophilic polymer contained in the upper layer is soluble in an organic solvent that does not dissolve the hydrophilic polymer contained in the underlying layer.
Claim 12
Independent Claim
83 words
A method for producing a biosensor comprising the steps of: forming a first layer containing an enzyme and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode which are provided on said insulating base; and forming a second layer containing a buffer on said first layer by using an organic solvent as the medium that does not dissolve said hydrophilic polymer.
Claim 13
Dependent Claim
72 words
The method for producing a biosensor in accordance with claim 12, wherein said step of forming the first layer comprises spreading an aqueous solution which dissolves the enzyme and the hydrophilic polymer on the insulating base and drying the spread solution, and wherein said step of forming the second layer comprises spreading a solution obtained by dispersing the buffer in an organic solvent solution of a lipid and drying the spread solution.
Claim 14
Dependent Claim
77 words
The method for producing a biosensor in accordance with claim 12, wherein said step of forming the first layer comprises spreading an aqueous solution which dissolves the enzyme and the hydrophilic polymer on the insulating base and drying the spread solution, and wherein said step of forming the second layer comprises spreading a solution obtained by dispersing the buffer in an organic solvent solution of the hydrophilic polymer on the fist layer and drying the spread solution.
Claim 15
Independent Claim
90 words
A method for producing a biosensor comprising the steps of: forming a first layer containing a buffer and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode provided on said insulating base; and forming a second layer containing a hydrophilic polymer and an enzyme on said first layer by using an organic solvent as the medium that does not dissolve said hydrophilic polymer contained in the first layer.
Claim 16
Dependent Claim
87 words
The method for producing a biosensor in accordance with claim 15, wherein said step of forming the first layer comprises spreading an aqueous solution which dissolves the buffer and the hydrophilic polymer on the insulating base and drying the spread solution, and wherein said step of forming the second layer comprises spreading an organic solvent solution of the hydrophilic polymer on the first layer and drying the spread solution, and further dropping an aqueous solution of the enzyme on the second layer and drying the dropped solution.
Claim 17
Dependent Claim
30 words
The method for producing a biosensor in accordance with claim 13 or 14, wherein said aqueous solution which dissolves the enzyme and the hydrophilic polymer further dissolves an electron acceptor.
Claim 18
Dependent Claim
23 words
The method for producing a biosensor in accordance with claim 16, wherein said aqueous solution of the enzyme further dissolves an electron acceptor.
Claim 19
Dependent Claim
40 words
The method for producing a biosensor in accordance with claim 14 or 16, further comprising a step of forming a third layer by spreading an organic solvent solution of a lipid over the second layer and drying the spread solution.
Claim 20
Independent Claim
77 words
A method for producing a biosensor comprising: a first step of spreading an aqueous solution containing a hydrophilic polymer and a buffer on an insulating base in close contact with an electrode system including a working electrode and a counter electrode provided on a face of said insulating base and drying the spread solution, and a second step of spreading an organic solvent solution containing at least an enzyme over the layer and drying the spread solution.
Claim 21
Dependent Claim
37 words
The method for producing a biosensor in accordance with claim 20, wherein either of said aqueous solution employed in said first step or the organic solvent solution employed in said second step further contains an electron acceptor.
Claim 22
Dependent Claim
28 words
The method for producing a biosensor in accordance with claim 20 or 21, wherein the organic solvent solution employed in said second step further contains a hydrophilic polymer.
References Cited (Backward Citations)
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