EP0617282A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Dry slide for diagnostic tests
Assignee: Difco Laboratories Inc
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Filing: 1994-03-14
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Granted: —
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Expires: 2014-03-14
Abstract
Abstract A test slide (10) for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives. The test slide (10) comprises a support strip (14), a coating (34) comprising a carrier and a diagnostic reagent disposed on the support strip (14), and a mount (12) comprising a front wall (18) and a rear wall (20). The coating (34) has the properties of being dry and stable, and water soluble. Further, the coating (34) and the diagnostic reagent are capable of rehydration. The support strip (14) is disposed between the front wall (18) and the rear wall (20) of the mount (12). Both the front (18) and rear (20) walls can have coinciding openings (28,28a) that overlie the support strip (14) thereby allowing spectroscopic, visual and microscopic examination of the support strip (14).
Inventors
- 👤 Ruth F. Eden
- 👤 Amy T. Meszaros
- 👤 Jerry W. Smith
- 👤 Leon F. Strenkoski
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 15:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
145 words
A test slide for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives, said test slide (10) comprising: a support strip (14) being dimensionally stable and having a top surface and a bottom surface, a coating (34) directly adhering to said support strip (14), said coating (34) comprising a carrier and a diagnostic reagent, said coating (34) being dry and stable, and water soluble, said coating (34) and said reagent being capable of rehydration, a mount (12) comprising a front wall (18) and a rear wall (20), said support strip (14) being disposed between said front wall (18) and said rear wall (20), and characterized by said front (18) and rear (20) walls having at least one coinciding opening (28,28a) that overlie said support strip (14) thereby allowing spectroscopic, visual and microscopic examination of said support strip (14).
Claim 2
Dependent Claim
145 words
A test slide for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives, said test slide (10) comprising: a support strip (14) being dimensionally stable and having a top surface and a bottom surface, a coating (34) directly adhering to said support strip (14), said coating (34) comprising a carrier and a diagnostic reagent, said coating (34) being dry and stable, and water soluble, said coating (34) and said reagent being capable of rehydration, a mount (12) comprising a front wall (18) and a rear wall (20), said support strip (14) being disposed between said front wall (18) and said rear wall (20), and characterized by said front (18) and rear (20) walls having at least one coinciding opening (28,28a) that overlie said support strip (14) thereby allowing spectroscopic, visual and microscopic examination of said support strip (14).
Claim 3
Dependent Claim
15 words
The test slide (10) of claim 1 further characterized by having four coinciding openings (28,28a).
Claim 4
Dependent Claim
34 words
The test slide (10) of claim 2 further characterized by having at least one of said openings (28) formed in said front wall (18) without said coinciding opening (28a) in said rear wall (20).
Claim 5
Dependent Claim
58 words
The test slide (10) of claim 1 wherein said reagent being capable of rehydration at room temperature by application of a plurality of cells, enzyme or metabolite to the coating (with or without the addition of water) to the coating such that it changes its visual or microscopic properties upon exposure to the specific cells, enzyme or metabolite.
Claim 6
Dependent Claim
15 words
The test slide (10) of claim 1 further characterized by a plurality of diagnostic reagents.
Claim 7
Dependent Claim
75 words
The test slide (10) of claim 1 wherein said diagnostic reagent is selected from the group consisting of a reagent for detecting: oxidase, beta lactamase, L-aniline amino peptidase (Gram reactions), indole, catalase, coagulase, urease, H 2 S, Voges-Proskauer, bile esculin, phosphatase, B-glucosidase, citrate utilization, nitrate, C 8 Esterase activity, PYR, glucuronidase activity, antibodies, antigen-antibody reactions, latex test, cellular staining, antibiotic sensitivity, high level aminoglycoside resistance, other cellular metabolites and enzymes, drug sensitivity, toxins and agglutination.
Claim 8
Dependent Claim
32 words
The test slide (10) of claim 6 wherein aid plurality of diagnostic reagents consists of said diagnostic reagent for detecting Gram staining and said diagnostic reagent for detecting glucuronidase reaction of bacteria.
Claim 9
Dependent Claim
23 words
The test slide (10) of claim 1 further characterized by said support strip (14) being composed of glass, plastic material or paper material.
Claim 10
Dependent Claim
22 words
The test slide (10) of claim 8 further characterized by said support strip (14) being in the form of a plastic film.
Claim 11
Dependent Claim
31 words
The support strip (14) of claim 8 wherein said plastic material is selected from the group consisting of polyethylene, polyester, polyvinyl chloride, polyethylene terephthalate, polyethylene terephthalate glycol, cellulose triacetate, and polycarbonate.
Claim 12
Dependent Claim
32 words
The support strip (14) of claim 8 wherein said paper material is selected from the group consisting of absorbent paper, plastic-backed paper, membrane covered absorbent paper, and porous membrane covered absorbent paper.
Claim 13
Dependent Claim
37 words
The test slide (10) of claim 1 further characterized by said coating (34) being applied to said entire top surface, spot dispensed onto said top surface of said support strip (14) or impregnating said support strip (14).
Claim 14
Dependent Claim
38 words
The test slide (10) of claim 1 wherein said carrier is selected from the group consisting of gelatin, polygalacturonic acid, pectin, agar, agarose, cellulose, carboxymethyl cellulose, guar, xanthan, acacia, plant gums, starch, polyvinyl alcohol, polyvinyl chloride and polyacrylamide.
Claim 15
Dependent Claim
58 words
The test slide (10) of claim 1 wherein said front (18) and rear (20) walls include opposing internal surfaces (24,26), further characterized by an outer margin of said support strip (14) attached to said mount (12) by an adhesive (22) disposed on either or both opposing internal surfaces (24,26) at least in an area surrounding said openings (28,28a).
Claim 16
Dependent Claim
45 words
The test slide (10) of claim 14 further characterized by said adhesive (22) covering said opposing internal surfaces (24,26) such that said internal surfaces (24,26) are bound to one another and thereby said front (18) and rear (20) walls are securely fastened to each other.
Claim 17
Dependent Claim
39 words
The test slide (10) of claim 14 further characterized by a clasp holding said opposing internal surfaces (24,26) contiguous such that said front (18) and rear (20) walls are securely fastened with the ability to open said mount (12).
Claim 18
Dependent Claim
51 words
The test slide (10) of claim 1 wherein said front (18) and rear (20) walls include opposing internal surfaces (24,26), further characterized by a clasp holding said opposing internal surfaces (24,26) contiguous with said support strip (14) sandwiched between such that said front (18) and rear (20) walls are securely fastened.
Claim 19
Dependent Claim
27 words
The test slide (10) of claim 1 wherein said mount (12) is of a semi-rigid material selected from the group consisting of plastic material and cardboard material.
Claim 20
Independent Claim
160 words
A method of making a test slide (10) for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives, said method comprising: forming a support strip (14) being dimensionally stable and having a top surface and a bottom surface, preparing a coating (34) comprising a carrier and a diagnostic reagent being dry and stable, water soluble, and capable of rehydration, bonding the coating (34) by its own properties to the support strip (14), mounting the support strip (14) in a mount (12) comprising a front wall (18) and a rear wall (20), the support strip (14) being disposed between the front wall (18) and the rear wall (20), and placing at least one coinciding openings (28,28a) in the front wall (18) and the rear wall (20) that overlie the support strip (14) such that spectroscopic, visual and microscopic examination of changes in the diagnostic reagent disposed on the support strip (14) can be monitored.
Claim 21
Independent Claim
160 words
A method of making a test slide (10) for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives, said method comprising: forming a support strip (14) being dimensionally stable and having a top surface and a bottom surface, preparing a coating (34) comprising a carrier and a diagnostic reagent being dry and stable, water soluble, and capable of rehydration, bonding the coating (34) by its own properties to the support strip (14), mounting the support strip (14) in a mount (12) comprising a front wall (18) and a rear wall (20), the support strip (14) being disposed between the front wall (18) and the rear wall (20), and placing at least one coinciding openings (28,28a) in the front wall (18) and the rear wall (20) that overlie the support strip (14) such that spectroscopic, visual and microscopic examination of changes in the diagnostic reagent disposed on the support strip (14) can be monitored.
Claim 22
Dependent Claim
34 words
The method of claim 19 wherein said step of bonding the coating (34) includes applying the coating to the entire top surface, spot dispensed onto the top surface, or impregnating the support strip (14).
Claim 23
Independent Claim
15 words
The method of claims 19 or 20 further characterized by forming four coinciding openings (28,28a).
Claim 24
Dependent Claim
31 words
The method of claim 21 further characterized by forming at least one of said openings (28) in said front wall (18) without said coinciding opening (28a) in said rear wall (20).
Claim 25
Dependent Claim
65 words
The method of claim 19 to 22 wherein said step of mounting the support strip (14) includes forming front (18) and rear (20) walls having opposing internal surfaces (24,26), and an outer margin of the support strip (14) attached to the mount (12) by an adhesive (22) disposed on either or both opposing internal surfaces (24,26) at least in an area surrounding the openings (28,28a).
Claim 26
Dependent Claim
44 words
The method of claim 22 further characterized by covering the opposing internal surfaces (24,26) with the adhesive (22) such that the internal surfaces (24,26) are bound to one another and thereby said front (18) and rear (20) walls are securely fastened to each other.
Claim 27
Dependent Claim
30 words
The method of claim 22 further characterized by holding the opposing internal surfaces (24,26) contiguous such that said front (18) and rear (20) walls are securely fastened by a clasp.
Claim 28
Dependent Claim
19 words
The method of claim 24 wherein said front (18) and rear (20) walls are securely fastened by a clasp.
Claim 29
Independent Claim
82 words
The method of any of claims 19 to 26 further characterized by including the step of applying a cell, its enzymes, metabolites or other cellular derivatives on the coating (34) on the support strip (14) through the opening (28,28a) adjacent the top surface of the support strip (14) through the opening (28,28a) adjacent the top surface of the support strip (14), and observing any spectroscopic, visible or microscopic changes which occur indicating the diagnostic reagent has changed in response to the application.
Claim 30
Dependent Claim
49 words
A kit for identification of a cell type, its enzymes, metabolites or other cellular derivatives comprising: modifying means for selecting specific cell types from a sample of inducing production of cellular enzymes, metabolites or other cellular derivatives, and a test slide according to any of claims 1 to 18.
References Cited (Backward Citations)
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