EP0541296A2
Expired / Lapsed
EP
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Device for optically discriminating kinds of bases
Assignee: Hamamatsu Photonics KK
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Filing: 1992-10-29
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Granted: —
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Expires: 2012-10-29
Abstract
Abstract According to this invention, a base is dropped onto a specular tape on run, whereby bases are applied onto the tape separate from one another. According to the first aspect of this invention, fluorescence is generated by a laser beam. Accordingly it is possible to consecutively discriminate bases. Because bases are applied to the specular tape, the reflected beam is never incident on the optical detecting device. The prepared specular tape is a recording medium which can perfectly secure a sequence of bases. According to the second aspect of this invention, a base is captured by a monoclonal antibody applied to the specular tape, and then a luminous antibody is supplied. Accordingly by providing different wavelengths of the luminescence for the anti-A, -G, -C and -T, bases can be discriminated from one another by the luminescence detection.
Inventors
- 👤 Shigeru c/o Hamamatsu Photonics K. K. Hosoi
- 👤 Tsuyoshi C/O Hamamatsu Photonics K. K. Hayakawa
- 👤 Hiroshi c/o Hamamatsu Photonics K. K. Tsuchiya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 23:44
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N21/84; G01N2021/845; G01N21/85; G01N2021/8557; G01N2021/8564; G01N21/6402
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
85 words
A device for optically discriminating bases in DNA, comprising: tape running means for running an elongate light reflecting specular tape lengthwise at a constant speed; base dropping means for separating a base from a single fragment of DNA sequentially and dropping the same onto a surface of the specular tape; light source for irradiating a beam to the specular tape at the downstream of a position where the base is dropped; and optical detecting means for detecting fluorescence of the base due to said beam.
Claim 2
Dependent Claim
39 words
A device for optically discriminating bases in DNA according to claim 1, wherein the tape running means includes an elongate light reflecting specular tape, and driving means for running the specular tape forwardly and backwardly in the longitudinal direction.
Claim 3
Dependent Claim
30 words
A device for optically discriminating bases in DNA according to claim 2, wherein the specular tape is a metal-vaporized tape or a tape of an organic material, and has flexibility.
Claim 4
Dependent Claim
44 words
A device for optically discriminating bases in DNA according to claim 2, wherein the specular tape is rolled before run, and after run, the specular tape is wound or left in its run state, depending on a length of a single fragment of DNA.
Claim 5
Dependent Claim
41 words
A device for optically discriminating bases in DNA according to claim 2, wherein the specular tape has collars provided on both sides, and when the specular tape is wound, the collars define a gap between each winding and its adjacent winding.
Claim 6
Dependent Claim
46 words
A device for optically discriminating bases in DNA according to claim 2, wherein a speed of the specular tape is so controlled that drops are dropped from the base dropping means to stay on the specular tape at an interval much wider than a dropping interval.
Claim 7
Dependent Claim
41 words
A device for optically discriminating bases in DNA according to claim 1, wherein the dropping means is a flow cell for giving fine vibrations to a liquid containing a single fragment of bases of DNA dropping the liquid in fine particles.
Claim 8
Dependent Claim
23 words
A device for optically discriminating bases in DNA according to claim 7, wherein the flow cell gives fine vibrations by a piezoelectric element.
Claim 9
Dependent Claim
36 words
A device for optically discriminating bases in DNA according to claim 7, where some of said fine particles contain bases sequentially severed from an end of said single fragment of DNA, and others contain no bases.
Claim 10
Dependent Claim
37 words
A device for optically discriminating bases in DNA according to claim 7, wherein said liquid contains an enzyme which sequentially separates bases constituting said single fragment of bases of DNA one by one from one end thereof.
Claim 11
Dependent Claim
37 words
A device for optically discriminating bases in DNA according to claim 7, wherein the flow cell drops the fine particles at a rate higher than a speed at which a base of the single fragment is severed.
Claim 12
Dependent Claim
34 words
A device for optically discriminating bases in DNA according to claim 1, wherein the single fragment of DNA to be introduced into the flow cell has the bases modified beforehand with identifiable fluorescent dye.
Claim 13
Dependent Claim
29 words
A device for optically discriminating bases in DNA according to claim 1, wherein the light source is a continuous laser or a pulse laser which emits short wavelength radiation.
Claim 14
Dependent Claim
35 words
A device for optically discriminating bases in DNA according to claim 1, wherein the light source is installed oblique to the specular tape to prevent reflected beams from directly incident on the optical detecting means.
Claim 15
Dependent Claim
34 words
A device for optically discriminating bases in DNA according to claim 1, wherein the optical detecting means includes as an optical detector a photon counting television camera with an optical fiber or a photomultiplier.
Claim 16
Dependent Claim
24 words
A device for optically discriminating bases in DNA according to claim 15, wherein the photomultiplier includes wavelength selecting filters on the light detecting surface.
Claim 17
Dependent Claim
29 words
A device for optically discriminating bases in DNA according to claim 15, wherein the optical detecting means includes electric storing means for storing electric outputs from the optical detector.
Claim 18
Independent Claim
167 words
A device for optically discriminating bases in DNA, comprising: tape running means for running an elongate light-reflecting specular tape lengthwise at a constant speed; base dropping means for separating a base from a single fragment of DNA and dropping the same onto a surface of the specular tape; monoclonal antibodies applying means for applying monoclonal antibodies corresponding to kinds of bases on the specular tape at the upstream of a position where the base is dropped; luminous antibodies supplying means for supplying luminous antibodies corresponding to kinds of the bases, combining with said monoclonal antibodies, to the specular tape at the downstream of the position where the base is dropped; washing means for washing said luminous antibodies, not combining with the bases, on the surface of the specular tape at the down stream of a position where the luminous antibodies are supplied; and detecting means for detecting luminescence of the base while supplying a luminescence reagent to the specular tape at the downstream of the washing position.
Claim 19
Dependent Claim
39 words
A device for optically discriminating bases in DNA according to claim 18, wherein the tape running means includes an elongate light reflecting specular tape, and driving means for running the specular tape forwardly and backwardly in the longitudinal direction.
Claim 20
Dependent Claim
30 words
A device for optically discriminating bases in DNA according to claim 19, wherein the specular tape is a metal-vaporized tape or a tape of an organic material, and has flexibility.
Claim 21
Dependent Claim
44 words
A device for optically discriminating bases in DNA according to claim 19, wherein the specular tape is rolled before run, and after run, the specular tape is wound or left in its run state, depending on a length of a single fragment of DNA.
Claim 22
Dependent Claim
40 words
device for optically discriminating bases in DNA according to claim 19, wherein the specular tape has collars provided on both sides, and when the specular tape is wound, the collars define a gap between each winding and its adjacent winding.
Claim 23
Dependent Claim
46 words
A device for optically discriminating bases in DNA according to claim 19, wherein a speed of the specular tape is so controlled that drops are dropped from the base dropping means to stay on the specular tape at an interval much wider than a dropping interval.
Claim 24
Dependent Claim
20 words
A device for optically discriminating bases in DNA according to claim 19, wherein the specular tape is run partially loosened.
Claim 25
Dependent Claim
41 words
A device for optically discriminating bases in DNA according to claim 18, wherein the dropping means is a flow cell for giving fine vibrations to a liquid containing a single fragment of bases of DNA dropping the liquid in fine particles.
Claim 26
Dependent Claim
23 words
A device for optically discriminating bases in DNA according to claim 25, wherein the flow cell gives fine vibrations by a piezoelectric element.
Claim 27
Dependent Claim
36 words
A device for optically discriminating bases in DNA according to claim 25, where some of said fine particles contain bases sequentially severed from an end of said single fragment of DNA, and others contain no bases.
Claim 28
Dependent Claim
37 words
A device for optically discriminating bases in DNA according to claim 25, wherein said liquid contains an enzyme which sequentially separates bases constituting said single fragment of bases of DNA one by one from one end thereof.
Claim 29
Dependent Claim
37 words
A device for optically discriminating bases in DNA according to claim 25, wherein the flow cell drops the fine particles at a rate higher than a speed at which a base of the single fragment is severed.
Claim 30
Dependent Claim
35 words
A device for optically discriminating bases in DNA according to claim 18, wherein the single fragment of DNA to be introduced into the flow cell has the bases modified beforehand with a identifiable fluorescent dye.
Claim 31
Dependent Claim
42 words
A device for optically discriminating bases in DNA according to claim 18, wherein the monoclonal antibodies applying means applies monoclonal antibodies to the entire surface of the specular tape, whereby monoclonal antibodies corresponding to the respective bases stay on the specular tape.
Claim 32
Dependent Claim
29 words
A device for optically discriminating bases in DNA according to claim 18, wherein the luminous antibodies supplying means are selective of the bases specifically bonded with the monoclonal antibodies.
Claim 33
Dependent Claim
21 words
A device for optically discriminating bases in DNA according to claim 18, wherein the washing means washes off nonbonded luminous antibodies.
Claim 34
Dependent Claim
42 words
A device for optically discriminating bases in DNA according to claim 18, wherein the optical detecting means includes a luminescence reagent supply means for supplying the luminescence reagent onto the specular tape, and luminescence detector for detecting luminescence corresponding to the bases.
Claim 35
Dependent Claim
26 words
A device for optically discriminating bases in DNA according to claim 34, wherein the optical detecting means has wavelength selecting filters on the light detecting surface.
Claim 36
Dependent Claim
25 words
A device for optically discriminating bases in DNA according to claim 34, wherein the luminescence detector includes electric storing means for storing electric outputs therefrom.
Claim 37
Dependent Claim
61 words
Apparatus for distinguishing bases cleaved from a nucleic acid, the apparatus comprising: means for driving a specularly reflective tape; means for applying a plurality of bases cleaved from a nucleic acid to a surface of the tape; means for sequentially causing the bases applied to the tape to luminesce or fluoresce; and means for detecting the resultant luminescent or fluorescent light.
Claim 38
Dependent Claim
54 words
Method for distinguishing bases cleaved from a nucleic acid, the method comprising the steps of: driving a specularly reflective tape; applying a base cleaved from a nucleic acid to a surface of the tape; causing sequentially the bases applied to the tape to luminesce or fluoresce; and detecting the resultant luminescent or fluorescent light.
References Cited (Backward Citations)
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