EP0537761B1
Expired / Lapsed
EP
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A biosensor and a method for measuring a concentration of a substrate in a sample
Assignee: Panasonic Holdings Corp
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Filing: 1992-10-16
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Granted: 1997-08-27
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Expires: 2012-10-16
Abstract
No abstract record available.
Inventors
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 05:23
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/902; G01N2333/904; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
81 words
A biosensor comprising: an electrical insulating substrate (1), a main electrode system (19) formed on the substrate (1) and having a working electrode (6) and a counter electrode (7), a reaction layer (5;50;51;52) provided in contact with or in the vicinity of the main electrode system (19) and containing an oxidoreductase, and a sub electrode system (20) as a reference provided with an interval from the main electrode system (19) and having a working electrode (8) and a counter electrode (9).
Claim 2
Dependent Claim
19 words
A biosensor according to claim 1, wherein the reaction layer (5;50;51;52) further contains electron acceptors and a hydrophilic polymer.
Claim 3
Dependent Claim
28 words
A biosensor according to claim 1 or 2, wherein a reference layer (25;53) containing electron acceptors and a hydrophilic polymer is provided on the sub electrode system (20).
Claim 4
Dependent Claim
38 words
A biosensor according to any of claims 1 to 3, wherein the oxidoreductase is selected from the group consisting of fructose dehydrogenase, glucose oxidase, alcohol oxidase, lactase oxidase, lactase dehydrogenase, cholesterol oxidase, xanthine oxidase and amino acid oxidase.
Claim 5
Dependent Claim
65 words
A biosensor according to claim 2, wherein the hydrophilic polymer is selected from the group consisting of carboxy methyl cellulose, hydroxy ethyl cellulose, hydroxy propyl cellulose, methyl cellulose, ethyl cellulose, ethyl hydroxy ethyl cellulose, carboxy methyl ethyl cellulose, polyvinylpyrrolidone, polyvinyl alcohol, gelatin or its derivatives, acrylic acid or its salts, methacrylic acid or its salts, starch or its derivatives, and maleic anhydride or its salts.
Claim 6
Dependent Claim
25 words
A biosensor according to claim 2, wherein the electron acceptors is selected from the group consisting of potassium ferricyanide, p-benzoquinone, phenazinemethosulfate, methylene blue and ferrocene.
Claim 7
Dependent Claim
43 words
A biosensor according to claim 1, wherein the main electrode system (19) and the sub electrode system (20) are provided on different surfaces of the substrate (1) from each other, and the reaction (50;51;52) layer is provided on the main electrode system (19).
Claim 8
Dependent Claim
30 words
A biosensor according to claim 1, wherein the counter electrode (7) of the main electrode system (19) is common to the counter electrode (9) of the sub electrode system (20).
Claim 9
Dependent Claim
23 words
A biosensor according to claim 1, wherein the main electrode system (19) is made from a material comprising carbon as a main component.
Claim 10
Dependent Claim
23 words
A biosensor according to claim 1, wherein the sub electrode system (20) is made from a material comprising carbon as a main component.
Claim 11
Dependent Claim
126 words
A biosensor comprising: an electrical insulating substrate (1), a main electrode system (19) formed on the substrate (1) and having a working electrode (6) and a counter electrode (7), a reaction layer (5;50;51;52) provided on the main electrode system (19) and containing an oxidoreductase and electron acceptors, and a sub electrode system (20) as a reference provided with an interval from the main electrode system (19) and the reaction layer (5;50;51;52) and having a working electrode (8) and a counter electrode (9), wherein a substrate contained in a sample liquid is quantified by reducing the electron acceptors by electrons generated in a reaction of the substrate contained in the sample liquid and the oxidoreductase and then by electrochemically measuring the amount of the reduced electron acceptors.
Claim 12
Independent Claim
157 words
A method for quantifying a substrate contained in a sample liquid by using a biosensor, the biosensor comprising an electrical insulating substrate (1), a main electrode system (19) formed on the substrate (1) and having a working electrode (6) and a counter electrode (7), a reaction layer (5;50;51;52) provided in contact with or in the vicinity of the main electrode system (19) and containing an oxidoreductase, and a sub electrode system (20) as a reference provided on the substrate (1) with an interval from the main electrode system (19) and having a working electrode (8) and a counter electrode (9), and the method comprising the steps of detecting a presence of the sample liquid on both the electrode systems (19,20) by detecting a change of electrical characteristics between the main electrode system (19) and the sub electrode system (20) and then applying a voltage to the main electrode system (19) and the sub electrode system (20), respectively.
Claim 13
Independent Claim
163 words
A method for quantifying a substrate contained in a sample liquid by using a biosensor, the biosensor comprising an electrical insulating substrate (1), a main electrode (19) formed on the substrate (1) and having a working electrode (6) and a counter electrode (7), a reaction layer (5;50;51;52) provided in contact with or in the vicinity of the main electrode system (19) and containing an oxidoreductase, and a sub electrode system (20) as a reference provided on the substrate (1) an interval from the main electrode system (19) and having a working electrode (8) and a counter electrode (9), and the method comprising the steps of detecting a change of electrical characteristics between the working electrode (6;8) and the counter electrode (7;9) in the main electrode system (19) and the sub electrode system (20), respectively and determining a nature of the sample liquid on the basis of the difference of time required for detecting the electrical characteristics in the main and sub electrode systems.
References Cited (Backward Citations)
No citation records indexed.
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