EP0537761A2
Expired / Lapsed
EP
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A biosensor and a method for measuring a concentration of a substrate in a sample
Assignee: Panasonic Holdings Corp
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Filing: 1992-10-16
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Granted: —
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Expires: 2012-10-16
Abstract
Abstract The present invention provides a biosensor comprising an electrical insulating substrate, a main electrode system formed on the substrate and having a working electrode and a counter electrode, a reaction layer provided in contact with or in the vicinity of the main electrode system and containing an oxidoreductase, and a sub electrode system as a reference provided with an interval from the main electrode system and having a working electrode and a counter electrode.
Inventors
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 14:46
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/902; G01N2333/904; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
70 words
A biosensor comprising: an electrical insulating substrate, a main electrode system formed on the substrate and having a working electrode and a counter electrode, a reaction layer provided in contact with or in the vicinity of the main electrode system and containing an oxidoreductase, and a sub electrode system as a reference provided with an interval from the main electrode system and having a working electrode and a counter electrode.
Claim 2
Dependent Claim
18 words
A biosensor according to claim 1, wherein the reaction layer further contains electron acceptors and a hydrophilic polymer.
Claim 3
Dependent Claim
24 words
A biosensor according to claim 1, wherein a reference layer containing electron acceptors and a hydrophilic polymer is provided on the sub electrode system.
Claim 4
Dependent Claim
34 words
A biosensor according to claim 1, wherein the oxidoreductase is selected from the group consisting of fructose dehydrogenase, glucose oxidase, alcohol oxidase, lactase oxidase, lactase dehydrogenase, cholesterol oxidase, xanthine oxidase and amino acid oxidase.
Claim 5
Dependent Claim
65 words
A biosensor according to claim 2, wherein the hydrophilic polymer is selected from the group consisting of carboxy methyl cellulose, hydroxy ethyl cellulose, hydroxy propyl cellulose, methyl cellulose, ethyl cellulose, ethyl hydroxy ethyl cellulose, carboxy methyl ethyl cellulose, polyvinylpyrrolidone, polyvinyl alcohol, gelatin or its derivatives, acrylic acid or its salts, methacrylic acid or its salts, starch or its derivatives, and maleic anhydride or its salts.
Claim 6
Dependent Claim
25 words
A biosensor according to claim 2, wherein the electron acceptors is selected from the group consisting of potassium ferricyanide, p-benzoquinone, phenazinemethosulfate, methylene blue and ferrocene.
Claim 7
Dependent Claim
38 words
A biosensor according to claim 1, wherein the main electrode system and the sub electrode system are provided on different surfaces of the substrate from each other, and the reaction layer is provided on the main electrode system.
Claim 8
Dependent Claim
26 words
A biosensor according to claim 1, wherein the counter electrode of the main electrode system is common to the counter electrode of the sub electrode system.
Claim 9
Dependent Claim
22 words
A biosensor according to claim 1, wherein the main electrode system is made from a material comprising carbon as a main component.
Claim 10
Dependent Claim
22 words
A biosensor according to claim 1, wherein the sub electrode system is made from a material comprising carbon as a main component.
Claim 11
Dependent Claim
114 words
A biosensor comprising: an electrical insulating substrate, a main electrode system formed on the substrate and having a working electrode and a counter electrode, a reaction layer provided on the main electrode system and containing an oxidoreductase and electron acceptors, and a sub electrode system as a reference provided with an interval from the main electrode system and the reaction layer and having a working electrode and a counter electrode, wherein a substrate contained in a sample liquid is quantified by reducing the electron acceptors by electrons generated in a reaction of the substrate contained in the sample liquid and the oxidoreductase and then by electrochemically measuring the amount of the reduced electron acceptors.
Claim 12
Dependent Claim
79 words
A biosensor comprising: an electrical insulating substrate having a plurality of surfaces, a plurality of electrode systems formed on at least two surfaces of the substrate, each of the electrode systems having a working electrode and a counter electrode, and a plurality of reaction layers containing an oxidoreductase, respectively, wherein the electrode systems are respectively provided on different surfaces of the substrate from each other, and the reaction layers are provided on the same surfaces as the electrode systems.
Claim 13
Dependent Claim
24 words
A biosensor according to claim 12, wherein kinds of the one or more oxidoreductases contained in reaction layers are different in every reaction layer.
Claim 14
Independent Claim
139 words
A method for quantifying a substrate contained in a sample liquid by using a biosensor, the biosensor comprising an electrical insulating substrate, a main electrode system formed on the substrate and having a working electrode and a counter electrode, a reaction layer provided in contact with or in the vicinity of the main electrode system and containing an oxidoreductase, and a sub electrode as a reference provided on the substrate with an interval from the main electrode system and having a working electrode and a counter electrode, and the method comprising the steps of detecting a presence of the sample liquid on both the electrode systems by detecting a change of electrical characteristics between the main electrode system and the sub electrode system and then applying a voltage to the main electrode system and a sub electrode system, respectively.
Claim 15
Independent Claim
149 words
A method for quantifying a substrate contained in a sample liquid by using a biosensor, the biosensor comprising an electrical insulating substrate, a main electrode system formed on the substrate and having a working electrode and a counter electrode, a reaction layer provided in contact with or in the vicinity of the main electrode system and containing an oxidoreductase, and a sub electrode system as a reference provided on the substrate with an interval from the main electrode system and having a working electrode and a counter electrode, and the method comprising the steps of detecting a change of electrical characteristics between the working electrode and the counter electrode in the main electrode system and the sub electrode system, respectively and determining a nature of the sample liquid on the basis of the difference of time required for detecting the electrical characteristics in the main and sub electrode systems.
References Cited (Backward Citations)
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