EP0534178A2
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EP
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Improved tissue glue prepared by using cryoprecipitate
Assignee: Omrix Biopharmaceuticals SA
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Filing: 1992-09-02
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Granted: —
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Expires: 2012-09-02
Abstract
Abstract A tissue glue is described comprising a component A which comprises a cryoprecipitate of whole blood and high an amount of a protease inhibitor corresponding to 3,000 to 5,000 KIU units of approtinin, and a component B comprising a proteolytic enzyme being capable of cleaving specifically fibrinogen present in component A and causing the formation of a fibrine polymer. In another embodiment an improved tissue glue is described comprising a component A which comprises a cryoprecipitate of whole blood, and a component B comprising a proteolytic enzyme obtainable from snake venom which enzyme is capable of cleaving specifically fibrinogen present in component A and causing the formation of a fibrine polymer.
Inventors
- 👤 Uri Dr. Martinowitz
- 👤 Frederic Bal
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 08:52
CPC Classifications
A; A61; A61L; A61L24/00; A61L24/04; A61L24/10; A61L24/106; A61L2/00; A61L2/16; A61L2/18; A61L24/0005; A61L2103/00; A61L2103/05
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
57 words
A tissue glue comprising a component A which comprises a cryoprecipitate of whole blood and high an amount of a protease inhibitor corresponding to 3,000 to 5,000 KIU units of aprotinin, and a component B comprising a proteolytic enzyme being capable of cleaving specifically fibrinogen present in component A and causing the formation of a fibrine polymer.
Claim 2
Dependent Claim
14 words
The tissue glue of claim 1 wherein the cryoprecipitate of component A is concentrated.
Claim 3
Dependent Claim
20 words
The tissue glue of claim 1 wherein the protease inhibitor is aprotinin in amounts of 3,000 to 5,000 KIU units.
Claim 4
Dependent Claim
23 words
The tissue glue of anyone of the claims 1 to 3 wherein the proteolytic enzyme is thrombin derived from mamals or human beings.
Claim 5
Dependent Claim
46 words
A tissue glue comprising a component A which comprises a cryoprecipitate of whole blood, and a component B comprising a proteolytic enzyme obtainable from snake venom which enzyme is capable of cleaving specifically fibrinogen present in component A and causing the formation of a fibrine polymer.
Claim 6
Dependent Claim
25 words
The tissue glue of claim 5 wherein the snake venom enzyme is batroxobin isolated from the venom of the South American pit viper Bothrpos Moujeni.
Claim 7
Dependent Claim
27 words
The tissue glue of claims 5 or 6 having high an amount of a protease inhibitor, preferably aprotinin, corresponding to 3,000 to 5,000 KIU units of aprotinin.
Claim 8
Dependent Claim
17 words
The tissue glue of anyone of the claims 1 to 7 wherein the cryoprecipitate is virus inactivated.
Claim 9
Dependent Claim
58 words
Process for manufacturing a fibrin glue according to claims 1 to 8 having the steps of - manufacturing component A comprising the steps of preparing a cryo solution from cryoprecipitate, - a virus inactivation, - the removal of virucidal agents, - addition of the protease inhibitor and preparing an appropiate solution of a suitable protease as component B.
Claim 10
Dependent Claim
27 words
A tissue glue comprising as component A fibrinogen, fibronectin and factor XIII and a protease inhibitor corresponding to an aprotinin amount of from 3,000 to 5,000 KIU/ml.
Claim 11
Dependent Claim
21 words
The tissue glue of claim 10 wherein component B is a proteolytic enzyme according to claims 5 and/or 6 or thrombin.
Claim 12
Dependent Claim
25 words
A tissue glue comprising as component A fibrinogen, fibronectin and factor XIII and component B is a proteolytic enzyme according to claims 5 and/or 6.
Claim 13
Dependent Claim
31 words
Use of high an amount of aprotinin of from 3,000 to 5,000 KIU/ml in combination with cyroprecipitate or a combination of fibrinogen, fibronectin and factor XIII for preparing a tissue glue.
Claim 14
Dependent Claim
13 words
Use of a snake venom protease for the preparation of a tissue glue.
Claim 15
Dependent Claim
14 words
Use of batroxobin isolated from Bothrpos Moujeni for the preparation of a tissue glue.
References Cited (Backward Citations)
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