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EP0512769A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells

Assignee: Becton Dickinson and Co
•
Filing: 1992-05-01
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Granted: —
•
Expires: 2012-05-01
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Abstract

Abstract A device is provided, and a method, for collecting and processing tissue to produce an endothelial cell product having a vessel for rinsing, draining, digesting and isolating tissue. The vessel has a rinsing and digesting chamber for containing tissue during processing. An inlet in the rinsing and digesting chamber allows entry of rinsing solution and tissue from liposuction means. A drain chamber is in the vessel and is in fluid communication with the rinsing and digesting chamber. The drain chamber connects with a vacuum source. A screen in the vessel separates the rinsing and digesting chamber from the drain chamber to allow filtered transfer. An isolation chamber in the vessel is separated by another screen. A channel in fluid communication with the isolation chamber includes a pair of ports controlled by valve means to be selectively in fluid communication with the channel. After processing, the channel has a pellet of microvessel endothelial cells and the valve means may place the pellet of microvessel endothelial cells in fluid communication with the ports. The method includes providing the vessel, introducing tissue to be processed, orienting the vessel to screen the tissue, introducing an enzyme and agitating to digest the tissue, centrifuging the vessel to transfer the cells from the digested tissue, and isolating the cells for retrieval.

Inventors

  • 👤 Paul G. Alchas
  • 👤 Joseph A. Di Pisa

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-31 17:44
CPC Classifications
C; C12; C12M; C12M45/00; C12M45/09

Claims Analysis

Total Claims: 10
Claim 1
Independent Claim 50 words
A device for collecting and processing tissue to produce an endothelial cell product comprising: a vessel having a rinsing and digesting chamber, a drain chamber, and an isolation chamber which chambers allow the rinsing, digestion and processing of tissue to produce within the vessel under sterile conditions endothelial cell product.
Claim 2
Dependent Claim 20 words
The device of Claim 1 wherein the rinsing and digesting chamber is separated from the drain chamber by a screen.
Claim 3
Dependent Claim 20 words
The device of Claim 2 wherein the rinsing and digesting chamber is separated from the isolation chamber by a screen.
Claim 4
Dependent Claim 42 words
The device of Claim 2 wherein said screen separates said rinsing and digesting chamber from said drain chamber and said isolation chamber, said drain chamber surrounds said rinsing and digesting chamber; and said drain chamber has a connection to a vacuum source.
Claim 5
Dependent Claim 37 words
The device of Claim 4 wherein the rinsing and digesting chamber includes an inlet for connection in fluid communication with a source of fat; said connection being on the side of said screen opposite said drain chamber.
Claim 6
Dependent Claim 47 words
The device of Claim 1 wherein the isolation chamber has a pair of ports connected by a channel, and valve means between the channel and each of the pair of ports to selectively control flow to and from said channel through each of the pair of ports.
Claim 7
Dependent Claim 39 words
The device of Claim 6 wherein the channel has a trap at the bottom thereof and the channel is shaped to collect therein a pellet of microvessel. endothelial cells and segregate red blood cells in the trap by centrifugation.
Claim 8
Dependent Claim 39 words
The device of Claim 1 wherein the vessel defines a cylindrical chamber with a frusto-conical end for housing the isolation chamber, and including a screen within the chamber for separating the rinsing and digesting chamber from the drain chamber.
Claim 9
Independent Claim 169 words
A method for collecting and processing tissue for producing an endothelial cell product comprising: providing a collecting vessel for processing tissue to produce an endothelial cell product, the vessel having a rinsing and digesting chamber, a drain chamber and an isolation chamber; introducing tissue to be processed into the rinsing and digesting chamber; introducing rinsing solution into the rinsing and digesting chamber; orienting the vessel to screen the tissue to be processed in the rinsing and digesting chamber from rinsing solution passed into the drain chamber; introducing an enzyme into the rinsing and digesting chamber; heating the tissue to be processed and the enzyme for a period of time and at a temperature sufficient to digest while agitating the rinsing and digesting chamber to digest the tissue with the enzyme, centrifuging the vessel to transfer the cells from the digested tissue from the rinsing and digesting chamber into the isolation chamber, and isolating the cells from the digested tissue in a channel as a pellet of microvessel endothelial cells.
Claim 10
Dependent Claim 33 words
The method of Claim 9 with the further step of isolating the cells from the digested tissue in a channel as a pellet of microvessel endothelial cells by application of a valve means.

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