EP0502589A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Kit for use in amplifying and detecting nucleic acid sequences
Assignee: F Hoffmann La Roche AG
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Filing: 1986-03-27
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Granted: —
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Expires: 2006-03-27
Abstract
Abstract The present invention is directed to a kit for the amplification and detection of at least one specific nucleic acid sequence in a sample, which kit comprises in packaged form, a multicontainer unit comprising: (a) primer for each different specific nucleic acid sequence being amplified and detected, selected so as to provide a primer substantially complementary to each strand of each specific sequence such that the extension product synthesized from one primer, when it is separated from its complement, serves as a template for synthesis of an extension product of the other primer; (b) means for synthesizing primer extension products; and (c) means for detecting the amplified sequence or sequences.
Inventors
- 👤 Kary Banks Mullis
- 👤 Norman Arnheim
- 👤 Randall Keichi Saiki
- 👤 Henry Anthony Erlich
- 👤 Glenn Thomas Horn
- 👤 Stephen Joel Scharf
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 15:40
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; B; B01; B01L; B01L7/00; B01L7/52; C07; C07K; C07K14/00; C07K14/795; C07K14/805; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6827; C12Q1/6844; C12Q1/6853; C12Q1/6858; C12Q1/686; B01J; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00495; B01J2219/00583; B01J2219/0059; B01J2219/00718; B01J2219/0072; B01J2219/00722; C12Q1/6876; C12Q1/6883; C12Q1/6886; C12Q1/6888; C12Q1/689; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
104 words
A kit for the amplification and detection of at least one specific nucleic acid sequence in a sample, which kit comprises in packaged form, a multicontainer unit comprising: (a) primer for each different specific nucleic acid sequence being amplified and detected, selected so as to provide a primer substantially complementary to each strand of each specific sequence such that the extension product synthesized from one primer, when it is separated from its complement, serves as a template for synthesis of an extension product of the other primer; (b) means for synthesizing primer extension products; and (c) means for detecting the amplified sequence or sequences.
Claim 2
Dependent Claim
36 words
A kit according to claim 1 characterized in that the primers are selected so as to be substantially complementary to the different strands of a specific nucleic acid sequence which is contained within a larger sequence.
Claim 3
Dependent Claim
56 words
A kit according to any one of claims 1 to 2 characterized in that the primers are selected so as to be substantially complementary to the different strands of a specific nucleic acid sequence which is DNA or RNA, including messenger RNA, which DNA or RNA may be single-stranded or double-stranded, or is a DNA-RNA hybrid.
Claim 4
Dependent Claim
38 words
A kit according to any one of claims 1 - 3 characterized in that the primers are selected so as to be substantially complementary to the different strands of a specific nucleic acid sequence which is genomic DNA.
Claim 5
Dependent Claim
44 words
A kit according to any one of claims 1 - 4 characterized in that it comprises a collection of primers for each strand of the specific nucleic acid being amplified and detected, at least one of said primers is substantially complementary to said strand.
Claim 6
Dependent Claim
24 words
A kit according to any one of claims 1 - 5 characterized in that the primers contain about 10 - 25 nucleotides or more.
Claim 7
Dependent Claim
45 words
A kit according to any one of claims 1 - 6 characterized in that at least one of the primers contains a nucleotide sequence attached to the 5'-end which sequence is non-complementary to the corresponding strand of the specific nucleic acid being amplified and detected.
Claim 8
Dependent Claim
48 words
A kit according to any one of claims 1 - 6 characterized in that at least one of the primers is interspersed with bases or a nucleotide sequence which bases or nucleotide sequence are non-complementary to the corresponding strand of the specific nucleic acid being amplified and detected.
Claim 9
Dependent Claim
34 words
A kit according to any one of claims 1 - 6 characterized in that each primer contains a restriction site which is the same as or different from a restriction site on another primer.
Claim 10
Dependent Claim
25 words
A kit according to any one of claims 1 - 9 characterized in that they further comprise an enzyme from the class known as helicases.
Claim 11
Dependent Claim
40 words
A kit according to any one of claims 1 - 10 comprising an enzyme selected from E. coli DNA polymerase I, Klenow fragment of E. coli DNA polymerase I, T4 DNA polymerase, another DNA polymerase, reverse transcriptase, and heat-stable enzymes.
Claim 12
Dependent Claim
15 words
A kit according to any one of claims 1 - 11 comprising readily detectable nucleotides.
Claim 13
Dependent Claim
27 words
A kit according to any one of claims 1 - 12 characterized in that the means for detecting the amplified sequence or sequences comprise a labelled probe.
Claim 14
Dependent Claim
58 words
The use of a kit according to any one of claims 1 - 13 for enabling detection and/or characterization of specific nucleic acid sequences associated with infectious diseases such as those caused by bacteria, viruses and protozoan parasites, genetic disorders such as those caused by specific deletions and/or mutations in genomic DNA or cellular disorders such as cancer.
References Cited (Backward Citations)
No citation records indexed.
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