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EP0501005A2
Expired / Lapsed
EP
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Flow imaging cytometer
Assignee: Sysmex Corp
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Filing: 1991-09-04
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Granted: —
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Expires: 2011-09-04
Abstract
Abstract A flow imaging cytometer, in which an image capturing area for cells traveling in the flow of a specimen solution is constantly monitored to perform cell photography in an efficient manner, is improved so that fluorescent images of cells can be acquired with greater efficiency. Cell particles to be sensed in the specimen are treated with a fluorescent stain, and the specimen solution is irradiated with light from a pulsed light source for exciting fluorescence, and with infrared light constantly for monitoring the passage of cells through the cytometer. When the monitoring light is made to serve also as the light for exciting fluorescence, the specimen flow is continuously irradiated at all times at a low luminance for the purpose of monitoring. Then, when a cell particle of interest is detected, the cell particle is irradiated with a light pulse of high luminance to obtain a still picture of the fluorescence-emitting cell. At detection of the cell, a source of white light can be actuated to acquire a white-light image of the cell.
Inventors
- 👤 Shinichi Ogino
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 11:12
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; G01N15/1425; G01N15/1427; G01N15/1456; G01N15/1459; G01N15/1434; G01N2015/144; G01N21/00; G01N21/17; G01N2021/1765; G01N2021/177; G01N2021/1772; G01N2021/1774; G01N2021/1789; G01N2021/1791; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6456
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
222 words
A flow imaging cytometer comprising: a flow cell formed to include a flat flow path for causing a specimen solution containing particle components to be sensed to flow as a flat stream; a first light source arranged on a first side of said flow cell for irradiating the specimen solution in said flow cell with pulsed light; first image capturing means arranged on a second side of said flow cell for capturing still pictures of the particle components in the specimen solution irradiated by said first light source; a second light source arranged on the first side of said flow cell for irradiating the specimen solution in said flow cell with light constantly; second image capturing means arranged on the second side of said flow cell for capturing images of the particle components irradiated by said second light source; processing means for executing prescribed analysis based upon image data from said first and second image capturing means; and control means for detecting the particle components based upon the image data from said second image capturing means, and on the basis of such detection, for actuating said first light source within an image capturing period of said first image capturing means, wherein the particle components to be sensed are treated with a fluorescent stain and said first light source is for exciting fluorescence.
Claim 2
Dependent Claim
16 words
The flow imaging cytometer according to claim 1, wherein said second light source emits infrared light.
Claim 3
Dependent Claim
19 words
The flow imaging cytometer according to claim 1, wherein said second light source emits exciting light for exciting fluorescence.
Claim 4
Dependent Claim
123 words
The flow imaging cytometer according to any of claims 1 through 3, further comprising: a third light source arranged on the first side of said flow cell for irradiating the specimen solution in said flow cell with pulsed white light; and third image capturing means arranged on the second side of said flow cell for capturing still pictures of the particle components in the specimen solution irradiated by said third light source; said control means detecting the particle components based upon the image data from said second image capturing means, and on the basis of such detection, for actuating said first and third light sources after respective prescribed time delays within image capturing periods of said first and third image capturing means respectively.
Claim 5
Dependent Claim
83 words
The flow imaging cytometer according to any of claims 1 through 3, wherein said first image capturing means has a two-dimensional image capturing area on the flow of the specimen solution, said second image capturing means has a linear image capturing area on the flow of the specimen solution, and the image capturing area of said second image capturing means is formed so as to cross the flow of the specimen solution within the image capturing area of said first image capturing means.
Claim 6
Dependent Claim
84 words
The flow imaging cytometer according to claim 4, wherein said first and third image capturing means each has a two-dimensional image capturing area on the flow of the specimen solution, said second image capturing means has a linear image capturing area on the flow of the specimen solution, and the image capturing area of said second image capturing means is formed so as to cross the flow of the specimen solution within the image capturing area of said first and third image capturing means.
Claim 7
Dependent Claim
28 words
The flow imaging cytometer according to claim 5 or 6, further comprising means for forming the irradiating light from said second light source into an elongated elliptical shape.
Claim 8
Dependent Claim
209 words
A flow imaging cytometer comprising: a flow cell formed to include a flat flow path for causing a specimen solution containing particle components to be sensed to flow as a flat stream; a first light source arranged on a first side of said flow cell for irradiating the specimen solution in said flow cell with light the quantity of which is switched; first image capturing means arranged on a second side of said flow cell for capturing still pictures of particle components in the specimen solution irradiated with high-luminance pulsed light from said first light source; second image capturing means arranged on a second side of said flow cell for capturing images of the particle components in the specimen solution irradiated continuously with low-luminance light from said first light source; processing means for executing prescribed analysis based upon image data from said first and second image capturing means; and control means for detecting the particle components based upon the image data from said second image capturing means, and on the basis of such detection, for switching said first light source over to irradiation with the high-luminance light, wherein the particle components to be sensed are treated with a fluorescent stain and said first light source is for exciting fluorescence.
Claim 9
Dependent Claim
118 words
The flow imaging cytometer according to claim 8, further comprising: a third light source arranged on a first side of said flow cell for irradiating the specimen solution in said flow cell with pulsed white light; and third image capturing means arranged on a second side of said flow cell for capturing still pictures of the particle components in the specimen solution irradiated by said third light source; said control means for detecting the particle components based upon the image data from said second image capturing means, and on the basis of such detection, for switching said first light source over to irradiation with the high-luminance light and actuating said third light source after respective prescribed time delays.
Claim 10
Dependent Claim
79 words
The flow imaging cytometer according to claim 8, wherein said first image capturing means has a two-dimensional image capturing area on the flow of the specimen solution, said second image capturing means has a linear image capturing area on the flow of the specimen solution, and the image capturing area of said second image capturing means is formed so as to cross the flow of the specimen solution within the image capturing area of said first image capturing means.
Claim 11
Dependent Claim
84 words
The flow imaging cytometer according to claim 9, wherein said first and third image capturing means each has a two-dimensional image capturing area on the flow of the specimen solution, said second image capturing means has a linear image capturing area on the flow of the specimen solution, and the image capturing area of said second image capturing means is formed so as to cross the flow of the specimen solution within the image capturing areas of said first and third image capturing means.
Claim 12
Dependent Claim
28 words
The flow imaging cytometer according to claim 10 or 11, further comprising means for forming the irradiating light from said first light source into an elongated elliptical shape.
References Cited (Backward Citations)
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