EP0494724A2
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EP
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Plasmids for controlling expression in plants
Assignee: Max Planck Institute for Molecular Genetics
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Filing: 1992-01-06
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Granted: —
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Expires: 2012-01-06
Abstract
Abstract Plasmids that comprise DNA sequences that permit the expression, controlled in respect of time and location, of a heterologous product in plants are described. The expression is not achieved until an inducer has been added. The DNA sequences include a functional combination of plant expression sequences and bacterial control sequences. Plants that comprise the DNA sequences of those plasmids are also described. Tetracycline is used as an inducer.
Inventors
- 👤 Christiane Dr. Gatz
- 👤 Claus Frohberg
- 👤 Astrid Kaiser
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-10 01:22
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8237; C12N15/8238
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
22 words
A plasmid that comprises means for controlling the time and the location of the expression of a DNA sequence in a plant.
Claim 2
Dependent Claim
16 words
A plasmid as claimed in claim 1, wherein the DNA sequence codes for a heterologous product.
Claim 3
Dependent Claim
31 words
A plasmid as claimed in claim 1 or claim 2, wherein the control means is such that expression of the DNA sequence is effected only in the presence of an inducer.
Claim 4
Dependent Claim
30 words
A plasmid as claimed in any one of claims 1 to 3, wherein the control means comprises a functional combination of a plant control sequence and one or more operators.
Claim 5
Dependent Claim
62 words
A plasmid as claimed in claim 4, wherein the control means comprises a plant promoter in which has been inserted one or more operator sequences, one or more genes for a repressor, the arrangement of the plant promoter, the operator(s) and the DNA sequence being such that the DNA sequence is expressed only in the presence of the inducer for the repressor.
Claim 6
Dependent Claim
22 words
A plasmid as claimed in claim 4 or claim 5, wherein the or each operator sequence is of prokaryotic or eukaryotic origin.
Claim 7
Dependent Claim
26 words
A plasmid as claimed in any one of claims 3 to 6, wherein the or each operator comprises the regulatory elements of a tetracycline resistance operon.
Claim 8
Dependent Claim
22 words
A plasmid as claimed in claim 7, wherein the operator comprises the regulatory elements of the tetracycline resistance operon of transposon Tn10.
Claim 9
Dependent Claim
27 words
A plasmid as claimed in any one of claims 3 to 8, wherein two operator sequences are located 3′ of the TATA element of the plant promoter.
Claim 10
Dependent Claim
36 words
A plasmid as claimed in any one of claims 1 to 9, which also comprises one or more of the following: a polylinker sequence, a polyadenylation signal and a DNA sequence coding for a selectable marker.
Claim 11
Dependent Claim
38 words
A plasmid as claimed in claim 9 or claim 10, which comprises a cauliflower mosaic virus 355 promoter having enhancer elements, a TATA box, two or three tet operators and a DNA sequence coding for a heterologous product.
Claim 12
Dependent Claim
61 words
A plasmid as claim in claim 11, which comprises a polylinker sequence, a 334 bp cauliflower mosaic virus 35S promoter fragment having enhancer elements, a 99 bp oligo-DNA fragment having a TATA-box and two tet opertors, a DNA sequence coding for a heterologous product, a 203 bp polyadenylation signal of the nopaline synthase gene and a 1650 bp hygromycin phosphotransferase gene.
Claim 13
Dependent Claim
38 words
A plasmid as claimed in claim 11 or claim 12, wherein the TATA box and the two tet operators are present, together with a linked CCC fragment, in a 99 bp oligo DNA fragment having the following sequence:
Claim 14
Dependent Claim
28 words
A plasmid as claimed in claim 11, wherein the TATA box and the three operators are present, together with a 83 bp DNA fragment having the following sequence:
Claim 15
Dependent Claim
4 words
Plasmid pAT2HyStu4 (DSM 6280).
Claim 16
Dependent Claim
4 words
Plasmid pAT2HyTriple-X (DSM ).
Claim 17
Dependent Claim
30 words
A plasmid that comprises a 526 bp cauliflower mosaic virus 35S promoter fragment, a 695 bp tet repressor fragment, and a 180 bp polyadenylation signal of the octopine synthase gene.
Claim 18
Dependent Claim
4 words
Plasmid pTET1 (DSM 6281).
Claim 19
Dependent Claim
19 words
A plant that comprises means for controlling the time and the location of the expression of a DNA sequence.
Claim 20
Dependent Claim
16 words
A plant as claimed in claim 19, wherein the DNA sequence codes for a heterologous product.
Claim 21
Dependent Claim
39 words
A plant as claimed in claim 19 or claim 20, wherein the control means is as defined in any one of claims 3 to 10 or comprises the control elements defined in any one of claims 11 to 13.
Claim 22
Dependent Claim
26 words
A plant as claimed in any one of claims 19 to 21, which comprises a plasmid as claimed in any one of claims 1 to 15.
Claim 23
Dependent Claim
27 words
A plant as claimed in any one of claims 19 to 22, which also comprises a DNA sequence that codes for the repressor for the operator present.
Claim 24
Dependent Claim
27 words
A plant as claime in any one of claims 19 to 23, wherein the operator is a tet operator and the repressor is a tet repressor protein.
Claim 25
Dependent Claim
19 words
A plant as claimed in claim 24, which comprises a plasmid as claimed in claim 17 or claim 18.
Claim 26
Dependent Claim
17 words
A plant which comprises plasmid pTET1 (DSM 6281), plasmid pAT2HyStu4 (DSM 6280) and plasmid pAT2HyTriple-X (DSM ).
Claim 27
Dependent Claim
17 words
A plant as claimed in any one of claims 19 to 26, which is a tobacco plant.
Claim 28
Dependent Claim
33 words
Use of a plasmid as claimed in any ony of claims 1 to 14 in production of plants in which expression of a specific DNA sequence is controllable in time and in location.
Claim 29
Dependent Claim
42 words
Use of a plasmid as claimed in any one of claims 1 to 14 and a plasmid coding for the corresponding repressor for the production of plants in which expression of a specific DNA sequence is controllable in time and in location
Claim 30
Dependent Claim
28 words
Use of plasmids pTET1, pAT2HyStu4 and pAT2Hy Triple-X for the production of plants in which expression of a specific DNA sequence is controllable in time and in location.
References Cited (Backward Citations)
No citation records indexed.
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