§
Luxuse
Patent Intelligence
Sign In
← Back to Patent Explorer
EP0488528A1 Expired / Lapsed EP ⏱️ 0.0 years remaining

Recombinant adeno-associated virus vectors

Assignee: Applied Immune Sciences Inc
•
Filing: 1991-10-29
•
Granted: —
•
Expires: 2011-10-29
📄 Original PDF 🌐 Google Patents 🔄 Sync Now

Abstract

Abstract A simplified method to produce recombinant adeno-associated virus (AAV) vectors is described. The procedure involves the use of chimeric plasmids which incorporate the Epstein Barr nuclear antigen (EBNA) gene, the latent origin of replication of Epstein Barr Virus (oriP), and a recombinant AAV genome. The chimeric plasmids themselves are also a part of the present invention. These EBV/AAV plasmids are maintained as multicopy extra-chromosomal elements in cells, such as human 293 cells. Permanent cell lines carrying these EBV/AAV plasmids are induced to produce large amounts of recombinant AAV virus upon addition of wild-type, adeno-associated virus helper functions. Recombinant AAV vectors produced in this manner are capable of transducing exogenous genes into other human cell lines and exhibit all of the attributes of viral elements produced by conventional methods.

Inventors

  • 👤 Jane Lebkowski
  • 👤 Maureen Mcnally
  • 👤 Thomas Okarma

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-03 22:12
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C07; C07K; C07K14/00; C07K14/005; C12N7/00; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16211; C12N2710/16222; C12N2710/16241; C12N2710/16244; C12N2710/16261; C12N2710/16262; C12N2750/00; C12N2750/00011; C12N2750/14011; C12N2750/14111; C12N2750/14122; C12N2750/14141; C12N2750/14143

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 48 words
A recombinant plasmid vector, comprising in the order given in the 5′-3′ direction: an Epstein Barr nuclear antigen (EBNA) gene, an Epstein Barr virus (EBV) latent origin of replication (oriP), and a recombinant adeno-associated virus (AAV) vector comprising exogenous genetic material and lacking a functional AAV rep gene.
Claim 2
Dependent Claim 14 words
The vector of Claim 1, wherein said plasmid vector comprises a detectible genetic marker.
Claim 3
Dependent Claim 14 words
The vector of Claim 2, wherein said genetic marker is an antibiotic resistance gene.
Claim 4
Dependent Claim 16 words
The vector of Claim 3, wherein said antibiotic resistance gene is located in said AAV vector.
Claim 5
Dependent Claim 20 words
The vector of Claim 3, wherein said antibiotic resistance gene is located in said plasmid vector outside said AAV vector.
Claim 6
Dependent Claim 39 words
The vector of Claim 1, wherein at least two genetic markers are present in said plasmid vector, an antibiotic resistance gene in said AAV vector and a second genetic marker located in said plasmid vector outside said AAV vector.
Claim 7
Dependent Claim 17 words
The vector of Claim 1, wherein said AAV vector further lacks a functioning structural capsid gene segment.
Claim 8
Dependent Claim 21 words
The vector of Claim 1, wherein said recombinant AAV vector comprises a selectable marker gene and two or more exogenous genes.
Claim 9
Independent Claim 86 words
A method for producing AAV vectors, which comprises: cloning a preformed AAV vector containing exogenous genetic material into an EBV plasmid, wherein said EBV plasmid comprises an EBNA gene, an oriP DNA fragment, and a detectible genetic marker; transfecting a host cell line with said EBV plasmid to produce a transfected cell line; growing said transfected cell line in a cell growth medium; contacting said transfected host cell with adenovirus and wild-type adeno-associated virus helper functions; and isolating said AAV vector from said cell growth medium.

References Cited (Backward Citations)

No citation records indexed.

USPTO Annuity & Maintenance Windows

Maintenance schedule is calculated for granted US utility patents.

Assigned Categories & Tags

No categories assigned to this patent yet.

Assign Category Tag