EP0473189A2
Expired / Lapsed
EP
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Multilayer analytical element for assaying fructosamine
Assignee: Arkray Inc
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Filing: 1991-08-30
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Granted: —
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Expires: 2011-08-30
Abstract
Abstract A multilayer analytical element for assaying fructosamine having a liquid-impermeable support, a buffer-containing layer which contains a buffer having pH of 8 to 12 and is formed on the support, a tetrazolium salt-containing layer which is formed on the buffer-containing layer and optionally an intermediate layer interposed between the buffer-containing layer and the tetrazolium salt-containing layer to prevent contact of these two layers, which element assays fructosamine in a short time with good accuracy.
Inventors
- 👤 Hisashi Sakamoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 16:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/66; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
42 words
A multilayer analytical element for assaying fructosamine comprising a liquid-impermeable support, a buffer-containing layer which contains a buffer having pH of 8 to 12 and is formed on said support and a tetrazolium salt-containing layer which is formed on said buffer-containing layer.
Claim 2
Dependent Claim
31 words
The multilayer analytical element according to claim 1, which further comprises an intermediate layer interposed between said buffer-containing layer and said tetrazolium salt-containing layer to prevent contact of said two layers.
Claim 3
Dependent Claim
14 words
The multilayer analytical element according to claim 1, wherein said buffer-containing layer contains uricase.
Claim 4
Dependent Claim
41 words
The multilayer analytical element according to claim 1, wherein a tetrazolium salt containing in said tetrazolium salt-containing layer is at least one selected from the group consisting of 3,3'-[3,3'-dimethoxy-(1,1'-biphenyl)-4,4'-diyl]-bis[2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 3,3'-[3,3'-dimethoxy-(1,1'-biphenyl)-4,4'-diyl]-bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride], 3,3'-(1,1'-biphenyl-4,4'-diyl)-bis(2,5-diphenyl-2H-tetrazolium chloride], 3-(p-iodophenyl)-2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and 2,5-diphenyl-3-(1-naphthyl)-2H-tetrazolium chloride.
Claim 5
Dependent Claim
16 words
The multilayer analytical element according to claim 1, a buffer has pH of 8.5 to 10.5.
References Cited (Backward Citations)
No citation records indexed.
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