EP0467349B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Regulation of gene expression by employing translational inhibition utilizing mRNA interfering complementary RNA
Assignee: Research Foundation of the State University of New York
•
Filing: 1984-10-19
•
Granted: 2000-12-27
•
Expires: 2004-10-19
Abstract
Abstract Gene expression of the genetic material of cellular material or an organism is regulated or inhibited by incorporating in or along with the genetic material of the cellular material or organisms DNA or other genetic material which transcribes to an RNA which is complementary to and capable of binding to the mRNA of the genetic material of said organism. Gene expression or regulation of a gene is controlled by expressing a DNA fragment or replica of said gene inserted or positioned after a promoter in the opposite direction relative to the gene so as to regulate or inhibit the expression of said gene.
Inventors
- 👤 Masayori Inouye
- 👤 Takeshi Mizuno
- 👤 Mei-Yin Chou
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 19:21
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C07; C07H; C07H21/00; A; A61; A61K; A61K38/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
71 words
A non-human organism containing a nucleic acid construct, said nucleic acid
construct being an artificial nucleic acid construct which, upon introduction into a
cell of said organism containing a gene, regulates the function of said gene and
produces a ribonucleotide sequence which does not naturally occur in the cell, is
complementary to at least a portion of a ribonucleotide sequence transcribed by
said gene, and regulates the function of said gene.
Claim 2
Dependent Claim
134 words
A non-human organism containing a nucleic acid construct, said nucleic acid
construct being an artificial nucleic acid construct which, upon introduction into a
cell of said organism containing a gene, regulates the function of said gene, and
which contains the following nucleic acid segments: (a) a transcriptional promotor segment; (b) a transcription termination segment; and (c) a segment of said gene, said gene segment located between said
promotor segment and said termination segment and being inverted with
respect to said promotor segment and said termination segment, the
polarity of said inverted gene segment being the same as that of said
promotor segment and said termination segment, whereby transcription of
the inverted gene segment occurs in a direction opposite to the direction of
transcription of the gene to thereby regulate the function of said gene.
Claim 3
Dependent Claim
40 words
The organism of any one of claims 1 to 2, wherein said nucleic acid construct or
nucleic acid sequence segment encodes a ribonucleotide sequence
complementary to a 5' end non-coding portion of said ribonucleotide acid
sequence transcribed by said gene.
Claim 4
Dependent Claim
39 words
The organism of any one of claims 1 to 2, wherein said nucleic acid construct or
nucleic acid sequence segment encodes a ribonucleotide sequence
complementary to a ribosome binding portion of said ribonucleotide acid
sequence transcribed by said gene.
Claim 5
Dependent Claim
39 words
The organism of any one of claims 1 to 2, wherein said nucleic acid construct or
nucleic acid sequence segment encodes a ribonucleotide sequence
complementary to the translation initiation portion of said ribonucleotide acid
sequence transcribed by said gene.
Claim 6
Dependent Claim
16 words
The organism of any one of claims 1 to 5, wherein said gene is an oncogene.
Claim 7
Dependent Claim
17 words
The organism of any one of claims 1 to 5, wherein said gene is a viral gene.
Claim 8
Dependent Claim
16 words
The organism of any one of claims 1 to 7, wherein said gene encodes a protein.
Claim 9
Dependent Claim
19 words
The organism of any one of claims 1 to 8, wherein said transcriptional promotor
segment comprises an inducible promotor.
Claim 10
Dependent Claim
22 words
The organism of any one of claims 1 to 9, wherein said gene segment includes
the 5' non-coding region of said gene.
Claim 11
Dependent Claim
22 words
The organism of any one of claims 1 to 10, wherein said gene segment includes
the ribosome binding portion of said gene.
Claim 12
Dependent Claim
22 words
The organism of any one of claims 1 to 11, wherein said gene segment includes
the translation initiation portion of said gene.
Claim 13
Dependent Claim
24 words
The organism of any one claims 1 to 12 containing said artificial nucleic acid
construct incorporated in or associated with the chromosomal genetic material.
Claim 14
Dependent Claim
28 words
The organism according to claim 13 containing said artificial nucleic acid
construct incorporated in or associated with the chromosomal genetic material in
the nucleus of its cellular material.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.