EP0454046A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Diagnostic test slide
Assignee: Difco Laboratories Inc
•
Filing: 1991-04-23
•
Granted: —
•
Expires: 2011-04-23
Abstract
Abstract A diagnostic test slide for performing diagnostic tests for detecting the presence of microorganisms, enzymes or metabolites comprising a plastic film (14) having a coating (34) therein comprising a carrier and a reagent. The coated film (14, 34) is placed in a mount (12) that is constructed and arranged to form a border around a portion of the film. A method is provided for making the test slide utilizing conventional, automated devices for making coated photographic films, and conventional, automated devices for mounting slide film in mounts.
Inventors
- 👤 Thomas E. Thompson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 11:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/26; C12Q1/34; C12Q1/37; G01N2333/00; G01N2333/795; G01N2333/80; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
168 words
A test slide for performing diagnostic tests for detecting the presence of microorganisms, their enzymes and metabolites, said test slide comprising a plastic film (14) having a top surface and a bottom surface, said plastic film (14) being dimensionally stable and impervious to liquids, a coating (34) bonded to the top surface of said film (14), said coating (34) comprising a carrier and a diagnostic reagent, said coating (34) having a high melting point, being dry and stable, and water soluble, said reagent being capable of rehydration such that it changes spectroscopic properties upon exposure to a specific microorganism, enzyme or metabolite, a mount (12) comprising a rear wall (20) and a front wall (18), said front wall (18) having an opening (28) therein, said coated film (14) being mounted between said rear wall (20) and said front wall (18) such that said opening (28) in said front wall (18) overlies said top surface of said film exposing a portion of the coating (34) through said opening (28).
Claim 2
Dependent Claim
23 words
The test slide set forth in claim 1 wherein said coating (28) is bonded to the entire top surface of said film (14).
Claim 3
Dependent Claim
38 words
The test slide set forth in claim 1 or 2 wherein said plastic film (14) is selected from the group consisting of polyethylene, PVC (polyvinyl chloride), PET (polyethylene terephthalate), PETG (polyethylene terephthalate glycol modified), polyester, and cellulose triacetate.
Claim 4
Dependent Claim
47 words
The test slide set forth in any of claims 1 to 3 wherein said carrier in said coating (34) is selected from the group consisting of gelatin, polygalacturonic acid, pectin, agar, agarose, cellulose, carboxymethyl cellulose, guar, xanthan, acacia, plant gums, starch, polyvinyl alcohol, polyvinyl chloride and polyacrylamide.
Claim 5
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting oxidase.
Claim 6
Dependent Claim
23 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting beta lactamase.
Claim 7
Dependent Claim
24 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting L-aniline amino peptidase.
Claim 8
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting indole.
Claim 9
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting catalase.
Claim 10
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting coagulase.
Claim 11
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting urease.
Claim 12
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting H₂S.
Claim 13
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting Voges-Proskauer.
Claim 14
Dependent Claim
23 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting bile esculin.
Claim 15
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting phosphatase.
Claim 16
Dependent Claim
22 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting B-glucosidase.
Claim 17
Dependent Claim
23 words
The test slide set forth in any of claims 1 to 4 wherein said diagnostic reagent comprises a reagent for detecting citrate utilization.
Claim 18
Dependent Claim
33 words
The test slide set forth in any of claims 1 to 17 wherein said coated film (14, 34) is bonded to said mount (12) at least in the area surrounding said opening (28).
Claim 19
Dependent Claim
97 words
The test slide set forth in any of claims 1 to 19 wherein said rear and front walls (18, 20) of said mount (12) each has outer and inner surfaces (24, 26), a layer (22) of pressure sensitive adhesive on the inner surface (24; 26) of said respective rear and front wall (18, 20), said coated film (14, 34) being positioned between said walls (18, 20) and having a configuration sucht that portions of said adhesive layers (22) bond to one another and to said film (14) to secure said plastic film (14) in said mount (12).
Claim 20
Dependent Claim
28 words
The test slide set forth in claim 19 wherein said mount (12) is of a semi-rigid material selected from the group consisting of plastic material and cardboard material.
Claim 21
Dependent Claim
15 words
The test slide set forth in claim 20 wherein said rear wall (20) is opaque.
Claim 22
Independent Claim
161 words
The method of making a test slide for performing diagnostic tests for detecting the presence of microorganisms, their enzymes and metabolites, said method comprising providing a plastic film (14) which is dimensionally stable and impervious to liquids, mixing a water soluble carrier and a water soluble diagnostic reagent for a specific microorganism, enzyme or metabolite, said reagent being capable of rehydration such that it changes spectroscopic properties upon exposure to a specific microorganism, enzyme or metabolite, coating said mixture on said film (14), drying said coated film creating a high melting point, dry and stable, coated film (34), capable of rehydration such that its spectroscopic properties change upon exposure to said specific microorganism, enzyme or metabolite, and mounting said coated film (34) in a mount (12) comprising a rear and front wall (18, 20), said front wall (18) having an opening (28) therein such that a portion of the coated surface (34) of the film is exposed through said opening (28).
Claim 23
Dependent Claim
27 words
The method set forth in claim 22 wherein said step of coating said film (14) comprises applying said mixture to the entire surface of said film (14).
Claim 24
Dependent Claim
34 words
The method set forth in claim 22 wherein said film (14) is selected from the group consisting of polyethylene, PVC (polyvinyl chloride), PET (polyethylene terephthalate), PETG (polyethylene terephthalate glycol modified), polyester, and cellulose triacetate.
Claim 25
Dependent Claim
42 words
The method set forth in claim 22 wherein said carrier in said coating (34) is selected from the group consisting of gelatin, polygalacturonic acid, pectin, agar, agarose, cellulose, carboxymethyl cellulose, guar, xanthan, acacia, plant gums, starch, polyvinyl alcohol, polyvinyl chloride and polyacrylamide.
Claim 26
Dependent Claim
13 words
The method set forth in claim 22 wherein said diagnostic reagent comprises oxidase.
Claim 27
Dependent Claim
18 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting beta lactamase.
Claim 28
Dependent Claim
19 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting L-aniline amino peptidase.
Claim 29
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting indole.
Claim 30
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting catalase.
Claim 31
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting coagulase.
Claim 32
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting urease.
Claim 33
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting H₂S.
Claim 34
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting Voges-Proskauer.
Claim 35
Dependent Claim
18 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting bile esculin.
Claim 36
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting phosphatase.
Claim 37
Dependent Claim
17 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting B-glucosidase.
Claim 38
Dependent Claim
18 words
The method set forth in claim 22 wherein said diagnostic reagent comprises a reagent for detecting citrate utilization.
Claim 39
Dependent Claim
39 words
The method set forth in claim 22 including the step of providing said coating (34) to said film (14) when the film (14) is in the form of a continuous strip and thereafter severing said strip into separate films.
Claim 40
Dependent Claim
99 words
The method set forth in claim 22 wherein said step of providing a mount (12) comprises a mount having rear and front walls (18, 20), each having outer and inner surfaces (24, 26), said step of bonding said substrate film (14) to said mount (12) comprises providing a layer (22) of pressure sensitive adhesive on the inner surface (24, 26) of said respective rear and front walls (18, 20) having a configuration such that portions of said adhesive layers (22) bond to one another and to said film (14) to secure said plastic film (14) in said mount (12).
Claim 41
Dependent Claim
26 words
The test slide set forth in claim 22 including the step of forming said mount (12) of a semi-rigid material of plastic material or cardboard material.
Claim 42
Independent Claim
54 words
The method set forth in any of claims 22-41 including the steps of applying a microorganism, its enzyme or metabolite on said coating (34) on said test slide through said opening (28); observing any spectroscopic change which occurs indicating said reagent has been changed due to the presence of said microorganism, enzyme or metabolite.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.