EP0442394A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Synthetic peptides specific for the detection of antibodies to HCV, diagnosis of HCV infection and prevention thereof as vaccines
Assignee: United Biomedical Inc
•
Filing: 1991-02-08
•
Granted: —
•
Expires: 2011-02-08
Abstract
Abstract The present invention relates to a method for the detection in body fluids of antibodies to hepatitis C virus (HCV), also known as a non-A non-B hepatitis (NANBH) virus and to the diagnosis of NANBH by the use of a composition of synthetic peptides. Each of these peptides has an amino acid sequence corresponding to immunodominant regions of a fusion protein and a non-structural polypeptide of HCV, SOD/HCV C100 and a postulated HCV structural (core) protein. More specifically, the present invention is directed to the use of a group of synthetic peptides in a prescribed sequence or their analogues for the detection of antibodies to HCV in body fluids. The detection method includes an enzyme-linked immunosorbent assay (ELISA), and other forms of immunoassay procedures. The present invention also relates to a method for generating high titer antibodies to HCV in healthy mammals, including humans, by the use of compositions containing these synthetic peptides, analogues or mixtures thereof, in a free, conjugated or polymeric form as key components in synthetic vaccines for the prevention of non-A non-B hepatitis (NANBH).
Inventors
- 👤 Chang Yi Wang
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 13:00
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61P; A61P1/00; A61P1/16; C07K16/00; C07K16/08; C07K16/10; C07K16/116; C07K16/118; A61K; A61K39/00; C07K2317/00; C07K2317/30; C07K2317/34; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24211; C12N2770/24222; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/82
Claims Analysis
Total Claims: 46
Claim 1
Independent Claim
35 words
A peptide composition comprising a peptide with an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 ; and (xi) analogues, segments, mixtures, combinations, conjugates and polymers thereof.
Claim 2
Dependent Claim
35 words
A peptide composition comprising a peptide with an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 ; and (xi) analogues, segments, mixtures, combinations, conjugates and polymers thereof.
Claim 3
Dependent Claim
33 words
A peptide composition according to Claim 1 comprising a combination of Peptides IE, IIG, IIIF and VE and having the amino acid sequence: wherein X is -OH or -NH 2 and analogues thereof.
Claim 4
Dependent Claim
35 words
A peptide composition according to Claim 1 comprising a segment of Peptide II and having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 and analogues thereof.
Claim 5
Dependent Claim
26 words
A peptide composition according to Claim 3 and having an amino acid sequence as follows: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 6
Dependent Claim
36 words
A peptide composition according to Claim 1 comprising a segment of Peptide IIIF and having an amino acid sequence selected from the group consisting of: wherein X is - OH or -NH 2 and analogues thereof.
Claim 7
Dependent Claim
26 words
A peptide composition according to Claim 5 and having an amino acid sequence as follows: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 8
Dependent Claim
31 words
A peptide composition according to Claim 1 comprising a combination of Peptides VIIIE and IXE and having the amino acid sequence: wherein X is -OH or -NH 2 and analogues thereof.
Claim 9
Dependent Claim
36 words
A peptide composition according to Claim 1 comprising a segment of Peptide VIIIE and having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 10
Dependent Claim
36 words
A peptide composition according to Claim 1 comprising a segment of Peptide IXE and having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 11
Dependent Claim
28 words
A peptide composition according to Claim 1 wherein the peptide has an amino acid sequence as follows: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 12
Dependent Claim
27 words
A peptide composition according to Claim 1 wherein the peptide has an amino acid sequence as follows: wherein X is -OH or -NH 2 and analogues thereof.
Claim 13
Dependent Claim
82 words
A method of detecting antibodies to hepatitis C virus (HCV) or diagnosis of HCV infection or NANBH comprising the steps: (i) Preparing a peptide composition according to Claim 1; (ii) Using an effective amount of the peptide composition as an antigen to form a complex with antibodies to HCV or NANBHV; (iii) Detecting the presence of the complex of peptide with antibodies to HCV or NANBHV by an enzyme linked immunosorbent assay, an immunoradiometric assay or an agglutination assay or other immunoassays.
Claim 14
Dependent Claim
17 words
A method according to Claim 12 where in the peptide composition is coated on a solid substrate.
Claim 15
Dependent Claim
33 words
A method according to Claim 13 wherein the step of detecting the presence of the complex of peptide with antibodies to HCV or NANBHV is by means of an enzyme linked immunosorbent assay.
Claim 16
Dependent Claim
29 words
A method according to Claim 12 wherein the method of detecting the presence of the complex of peptide with antibodies to HCV or NANBHV is by using an immunoradiometric-assay.
Claim 17
Dependent Claim
29 words
A method according to Claim 12 wherein the method of detecting the presence of the complex of peptide with antibodies to HCV or NANBHV is by an agglutination assay.
Claim 18
Dependent Claim
37 words
A method according to Claim 12 wherein the peptide composition is a combination of peptides IE, IIG, IIIF and VE and having the amino acid sequence: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 19
Dependent Claim
38 words
A method according to claim 12 wherein the peptide composition comprises a segment of Peptide II and has an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 and analogues thereof.
Claim 20
Dependent Claim
29 words
A method according to claim 12, wherein the peptide composition comprises a peptide having an amino acid sequence: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 21
Dependent Claim
38 words
A method according to claim 12 wherein the peptide composition is a segment of peptide IIIF and having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 and analogues thereof.
Claim 22
Dependent Claim
29 words
A method according to claim 12 wherein the peptide composition comprises a peptide having an amino acid sequence: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 23
Dependent Claim
34 words
A method according to Claim 12 wherein the peptide composition comprises a combination of Peptides VIIIE and IXE and has an amino acid sequence: wherein X is -OH or -NH 2 and analogues thereof.
Claim 24
Dependent Claim
32 words
A method according to Claim 12 wherein the peptide composition comprises a segment of Peptide VIIIE and has an amino acid sequence: wherein X is -OH or -NH 2 and analogues thereof.
Claim 25
Dependent Claim
39 words
A method according to Claim 12 wherein the peptide composition comprises a segment of Peptide IXE and has an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 26
Dependent Claim
31 words
A method according to Claim 12 wherein the peptide composition comprises a peptide having an amino acid sequence as follows: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 27
Dependent Claim
30 words
A method according to Claim 12 wherein the peptide composition comprises a peptide having an amino acid sequence as follows: wherein X is -OH or -NH 2 and analogues thereof.
Claim 28
Dependent Claim
46 words
Antibodies to HCV or NANBHV produced by using as an immunogen a peptide composition comprising a peptide with an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 ; and (xi) analogues, segments, mixtures, combinations, conjugates and polymers thereof.
Claim 29
Dependent Claim
46 words
Antibodies to HCV or NANBHV produced by using as an immunogen a peptide composition comprising a peptide with an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 ; and (xi) analogues, segments, mixtures, combinations, conjugates and polymers thereof.
Claim 30
Dependent Claim
89 words
An enzyme linked immunosorbent assay (ELISA) test kit for the detection of antibodies to HCV or NANBHV or the diagnosis of HCV or NANBHV infection comprising: (i) compartmented enclosure containing multiple wells coated with a peptide composition according to Claim 1; (ii) a negative control sample; (iii) an inactivated HCV positive control sample; (iv) specimen diluent comprising PBS buffer containing 20% by volume normal goat serum; 1% by weight gelatin and 0.05% by weight TWEEN 20; (v) peroxidase labelled antibodies to human IgG; and (vi) a color change indicator.
Claim 31
Dependent Claim
44 words
An ELISA test kit according to claim 28 wherein the multiple wells are coated with a peptide composition which is a combination of Peptide IE, IIG, IIIF and VE with an amino acid sequence: wherein X is -OH or -NH 2 and analogues thereof.
Claim 32
Dependent Claim
46 words
An ELISA test kit according to claim 28 wherein the multiple wells are coated with a peptide composition comprising a segment of Peptide II and having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 and analogues thereof.
Claim 33
Dependent Claim
32 words
An ELISA test kit according to claim 28 wherein the multiple wells are coated with a peptide having an amino acid sequence: wherein X is OH or -NH 2 and analogues thereof.
Claim 34
Dependent Claim
45 words
An ELISA test kit according to claim 28 wherein the multiple wells are coated with a peptide composition comprising a segment of Peptide IIIF having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 and analogues thereof.
Claim 35
Dependent Claim
23 words
An ELISA test kit according to claim 32 wherein the peptide is: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 36
Dependent Claim
41 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a combination of Peptide VIIIE and IXE having an amino acid sequence: wherein X is -OH or -NH 2 and analogues thereof.
Claim 37
Dependent Claim
46 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a segment of Peptide VIIIE having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 38
Dependent Claim
46 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a segment of Peptide IXE having an amino acid sequence selected from the group consisting of: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 39
Dependent Claim
39 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a peptide having an amino acid sequence as follows: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 40
Dependent Claim
38 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a peptide having an amino acid sequence as follows: wherein X is -OH or -NH 2 and analogues thereof.
Claim 41
Dependent Claim
47 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a mixture of Peptides IIH and VE, Peptides IIH and VE having the following amino acid sequences respectively: wherein X is -OH or -NH 2 or analogues thereof.
Claim 42
Dependent Claim
49 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a mixture of Peptides IIF, IIID and VE, Peptide IIF, IIID and VE having the following amino acid sequences respectively: wherein X is -OH or -NH 2 or analogues thereof.
Claim 43
Dependent Claim
50 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a mixture of Peptides IIH, VE and VIIIE, Peptide IIH, VE and VIIIE having the following amino acid sequences respectively: wherein X is -OH or -NH 2 or an analogue thereof.
Claim 44
Dependent Claim
47 words
An ELISA test kit according to Claim 28 wherein the multiple wells are coated with a peptide composition comprising a mixture of Peptides VIIIE and IXD, Peptide VIIIE and IXD having the following amino acid sequences respectively: wherein X is -OH or -NH 2 and analogues thereof.
Claim 45
Dependent Claim
67 words
A peptide which is immunoreactive to HCV with at least about 15 to not more than about 65 amino acids in a sequences having as a segment thereof said segment selected from the group consisting of: (i) Ser-Gly-Lys-Pro-Ala; (XII) (ii) Ile-Ile-Pro-Asp-Arg; (XIII) (iii) Glu-Glu-Cys-Ser-Gln; (XIV) (iv) His-Leu-Pro-Tyr-Ile; (XV) (v) Glu-Gln-Gly-Met-Met; (XVI) (vi) Leu-Ala-Glu-Gln-Phe; (XVII) (vii) Lys-Gln-Lys-Ala-Leu; (XVIII) (viii) Gly-Leu-Leu-Gln-Thr; (XIX) (ix) Glu-Val-Ile-Ala-Pro; and (XX) (x) Glu-Val-Leu-Tyr-Arg. (XXI)
Claim 46
Dependent Claim
67 words
A peptide which is immunoreactive to HCV with at least about 15 to not more than about 65 amino acids in a sequences having as a segment thereof said segment selected from the group consisting of: (i) Ser-Gly-Lys-Pro-Ala; (XII) (ii) Ile-Ile-Pro-Asp-Arg; (XIII) (iii) Glu-Glu-Cys-Ser-Gln; (XIV) (iv) His-Leu-Pro-Tyr-Ile; (XV) (v) Glu-Gln-Gly-Met-Met; (XVI) (vi) Leu-Ala-Glu-Gln-Phe; (XVII) (vii) Lys-Gln-Lys-Ala-Leu; (XVIII) (viii) Gly-Leu-Leu-Gln-Thr; (XIX) (ix) Glu-Val-Ile-Ala-Pro; and (XX) (x) Glu-Val-Leu-Tyr-Arg. (XXI)
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.