EP0442025A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Optical particle analyzing apparatus having two types of light sources
Assignee: Sysmex Corp
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Filing: 1990-06-21
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Granted: —
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Expires: 2010-06-21
Abstract
Abstract An optical particle analyzing apparatus includes a flow cytometer in which laser (10) light and lamp (26) light having a wavelength shorter than that of the laser (10) light are used simultaneously when particles in a blood specimen or the like are analyzed by the flow cytometer. Scattered light emitted by a particle due to irradiation with the laser (10) light and fluorescence emitted by the particle due to irradiation with the lamp (26) light are detected. A signal indicative of the scattered light is used as a timing signal to detect the fluorescence. This arrangement makes it possible to reduce the size and cost of the apparatus.
Inventors
- 👤 Masakazu Fukuda
- 👤 Hiroyuki Nakamoto
- 👤 Hiroyuki Seshimo
- 👤 Hidemichi Tohori
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 02:01
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N15/1459; G01N2015/1477; G01N21/00; G01N21/17; G01N2021/1789; G01N2021/1791; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N2021/6439; G01N21/47
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
242 words
An optical particle analyzing apparatus for classifying and enumerating particles, comprising: a flow cell; means for passing particles such as cells stained with a fluorescent stain through said flow cell in the form of a fine particle stream; means for irradiating an irradiating zone in the fine stream of particles in said flow cell with two types of light; means for detecting respective ones of scattered light and fluorescence produced by the particles in the irradiating zone; means for processing and analyzing signals outputted by said detecting means, wherein said two kinds of light which irradiate the irradiating zone are a laser light beam emitted by a laser light source and a lamp light beam, which has a wavelength shorter than that of the laser light beam, emitted by a lamp light source, the laser light beam and lamp light beam intersect each other at the irradiating zone to irradiate the same, and the irradiating laser light beam is narrowed to a beam narrower than the lamp light beam in a direction in which the particles flow; and a signal processor for signal-processing a scattered-light signal which results from the laser light beam and is detected in the irradiating zone, and a fluorescence signal which results from the lamp light beam and is detected in the irradiating zone, wherein when the fluorescence signal is signal-processed by said signal processor, the scattered-light signal is used as a timing signal for this signal processing.
Claim 2
Dependent Claim
15 words
The apparatus according to Claim 1, wherein the laser light source is a semiconductor laser.
Claim 3
Dependent Claim
61 words
The apparatus according to Claim 1, wherein the irradiating light beams composed of the laser light beam and lamp light beam intersect each other at an angle of approximately 90°, and forward-scattered light and side fluorescence which result from the laser light beam and lamp light beam, respectively, and are emitted in the same direction, are detected upon undergoing wavelength selection.
Claim 4
Dependent Claim
76 words
The apparatus according to Claim 1, wherein the irradiating light beams composed of the laser light beam and lamp light beam intersect each other at an angle of approximately 90°, and forward-scattered light which results from the laser light beam and is emitted in a first direction, as well as side-scattered light and back fluorescence which result from the laser light beam and lamp light beam, respectively, and are emitted in a second direction, is detected.
References Cited (Backward Citations)
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