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EP0435380A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

A chemical reaction pack and method of using same

Assignee: Eastman Kodak Co
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Filing: 1990-12-14
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Granted: —
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Expires: 2010-12-14
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Abstract

Abstract In techniques for detecting nucleic acid material, there is a risk of contamination during unstoppering of reaction containers and transfer of liquids used in such reactions. This is because aerosols are produced during such unstoppering and transfer, which may contain some amplified nucleic acid material. Described herein are improvements in a disposable chemical reaction pack (10) and method for examining fluids using such a pack, in which liquids can be reacted and examined without the need to unstopper the reaction container and transfer the liquid to a further container for examination. The pack (10) comprises a first chamber (20) into which liquid to be examined is first inserted and sealed. This chamber (20) is connected to a second chamber (24) in which the liquid is examined. A rupturable seal (34) is provided between the first and second chambers (20, 24), which allows liquid to flow from the first chamber (20) into the second chamber (24) once linear pressure has been applied to the liquid in the first chamber (20). Advantageously, an expansion chamber (22) is provided intermediate the first and second chambers to control the velocity of liquid transfer from the first chamber (20) to the second chamber (24).

Inventors

  • 👤 Thomas William C/O Eastman Kodak Comp. Glanville
  • 👤 Douglas Steven C/O Eastman Kodak Comp. Bensley

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-01 21:07
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; G; G01; G01N; G01N33/00

Claims Analysis

Total Claims: 5
Claim 1
Independent Claim 127 words
A disposable chemical reaction pack (10) comprising:- a sealed hollow body (12) formed from impermeable pliable sheet material; a first chamber (20) formed within the body (12), and arranged to receive liquid from a sample to be reacted; a second chamber (24) serially connected to the first chamber (20) and being normally open to receive fluid flow from the first chamber (20); and a rupturable seal (34) disposed between the first and second chambers (20, 24) for retaining the sample within the first chamber (20), the rupturable seal (34) being constructed so that it will rupture upon the application of pressure to the liquid in the first chamber (20); characterized in that the second chamber (24) includes an expansion chamber (22) located downstream from the seal (34).
Claim 2
Dependent Claim 24 words
A pack according to claim 1, wherein the rupturable seal (34) forms a wall between the first chamber (20) and the expansion chamber (22).
Claim 3
Dependent Claim 49 words
A pack according to claim 1 or 2, wherein the chambers (20, 22, 24) are disposed within the hollow body (12) such that liquid contained within the first chamber (20) can be progressively transferred from one chamber to another by applying external pressure linearly to the hollow body (12).
Claim 4
Dependent Claim 21 words
A pack according to any one of the preceding claims, wherein some of the chambers (20, 22, 24) have reagents therewithin.
Claim 5
Independent Claim 116 words
A method of examining a liquid comprising the steps of: sealing the liquid to be examined in the first chamber (20) of a chemical reaction pack (10) according to any one of the preceding claims; applying an external pressure to the first chamber (20) to cause the seal (34) to rupture and to allow at least part of the liquid to continuously transfer out from the first chamber (20) into the second chamber (24) through the expansion chamber (22); and examining the liquid in the second chamber (24); characterized in that the expansion chamber (22) acts to slow down the velocity of transfer of the liquid from the first chamber (20) to the second chamber (24).

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