EP0429907B1
Expired / Lapsed
EP
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Optical biosensor
Assignee: Bayer AG
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Filing: 1990-11-08
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Granted: 1994-06-01
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Expires: 2010-11-08
Abstract
Abstract The optical biosensor is based on fluorescence energy transfer and consists of a) a solid support, b) a monolayer or multilayer Langmuir-Blodgett(LB) film applied to a), c) at least one fluorescent dye F1 which is located in at least one of the upper 4 layers of the LB film, d) a molecule (receptor) which is capable of specific interaction and is bound or located in or on the uppermost layer of the LB film, and e) a mobile fluorescent dye F2 whose excitation band overlaps sufficiently for energy transfer with the emission band of F1 and which e1) is covalently bonded to a ligand which is able to bind to the receptor or which e2) is covalently bonded to another receptor which is able to bind to the complex of the first receptor and ligand, where the ligand or the ligand and the second receptor are not initially bound to the LB film.
Inventors
- 👤 Herbert Dr. Hugl
- 👤 Eberhard Dr. Kuckert
- 👤 Dietmar Dr. Möbius
- 👤 Holger Dr. Ohst
- 👤 Meinhard Dr. Rolf
- 👤 Hans Jürgen Dr. Rosenkranz
- 👤 Heinrich Christian Dr. Schopper
- 👤 Hans-Ulrich Dr. Siegmund
- 👤 Klaus Dr. Sommer
- 👤 Rolf Dr. Wehrmann
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 17:32
CPC Classifications
C; C07; C07D; C07D277/00; C07D277/02; C07D277/20; C07D277/32; C07D277/56; C07D311/00; C07D311/02; C07D311/04; C07D311/06; C07D311/08; C07D311/16; C07D417/00; C07D417/02; C07D417/04; G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/543; G01N33/54366; G01N33/54373; G01N33/551; G01N33/552; G01N33/553; G01N33/58; G01N33/582; G01N2021/6439; G01N2021/6441; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/80; Y10S436/805
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
154 words
Optical biosensor based on fluorescence energy transfer, consisting of a) a solid support, b) a single-layer or multilayer Langmuir-Blodgett (LB) film attached to a), c) at least one fluorescent dye F₁ which is located in at least one of the top 4 layers of the LB film, d) a molecule (receptor) which is capable of specific interaction and which is bound or located in or on the topmost layer of the LB film, and e) a mobile fluorescent dye F₂ whose excitation band overlaps, sufficiently for an energy transfer, with the emission band of F₁ and which e1) is covalently bonded to a ligand which is able to bind to the receptor, or which e2) is covalently bonded to another receptor which is able to bind to the complex composed of the first receptor and ligand, where the ligand or the ligand and the second receptor are initially not bound to the LB film.
Claim 2
Dependent Claim
49 words
Biosensor according to Claim 1, characterized in that the solid support used is composed of glass, quartz glass, Li niobate, zinc selenide, porcelain, semiconductor materials such as germanium, GaAs or silicon, a metal, a plastic or a metallized plastic, it being possible for the solid support to be surface-modified.
Claim 3
Dependent Claim
17 words
Biosensor according to Claim 1, characterized in that the matrix of the LB film is a polymer.
Claim 4
Dependent Claim
15 words
Biosensor according to Claim 1, characterized in that F₁ is covalently bonded to the matrix.
Claim 5
Dependent Claim
35 words
Biosensor according to Claim 1, characterized in that the dye F₁ is spread together with an amphiphilic matrix, preferably in that the spreading together is with a dye F₁ which forms disc aggregates (J aggregates).
Claim 6
Dependent Claim
26 words
Biosensor according to Claim 1, characterized in that the dye F₁ is located in at least one of the two upper layers of the LB film.
Claim 7
Dependent Claim
24 words
Biosensor according to Claim 1, characterized in that the receptor molecule is attached covalently, preferably via a spacer molecule, to the topmost LB film.
Claim 8
Dependent Claim
22 words
Biosensor according to Claim 1, characterized in that the receptor molecule is located onto the topmost film via a hydrophobic membrane anchor.
Claim 9
Dependent Claim
54 words
Biosensor according to Claim 1, characterized in that a ligand which is labelled with fluorescent dye or is self-fluorescent binds to the binding sites of the receptor molecules, or in that an analyte which is not fluorescent-labelled competitively displaces a fluorescent-labelled ligand which is initially bound to the binding sites of the receptor molecules.
Claim 10
Dependent Claim
56 words
Biosensor according to Claim 1, characterized in that a second receptor molecule which is labelled with the fluorescent dye F₂ and binds either specifically to the complex of receptor and analyte or to a different molecular region of the analyte than the receptor in the manner of a sandwich assay is added to the analyte solution.
References Cited (Backward Citations)
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