EP0425018A2
Expired / Lapsed
EP
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Method and formulation employing type II endoglycosidase
Assignee: Procter and Gamble Co
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Filing: 1990-10-16
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Granted: —
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Expires: 2010-10-16
Abstract
Abstract Methods and formulations for removing glycoside-containing substances from surfaces by treatment with Type II endoglycosidase alone or in combination with other enzymes and/or detergents.
Inventors
- 👤 Richard Shepard Carpenter
- 👤 Pushkaraj Jogannath Lad
- 👤 Irwin J. Goldstein
- 👤 Ann Margaret Wolff
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 16:13
CPC Classifications
C; C11; C11D; C11D1/00; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38645; A; A61; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/49; A61K8/4906; A61K8/4933; A61K8/64; A61K8/66; A61Q; A61Q11/00; A61Q11/02; A61Q15/00; A61Q17/00; A61Q17/04; A61Q5/00; A61Q5/02; C11D3/0005; C11D3/0078; C11D3/38636
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
101 words
A method for releasing at least a portion of a substance, preferably a glycoprotein-containing substance, more preferably a Type II endoglycosidase reactive substance, from a surface to which it is bound other than by an immunological bond, said substance having a proximal portion bound to said surface, a distal portion extending outwardly from said proximal portion, and a linkage disposed at the juncture of said proximal and distal portions which is reactive with a Type II endoglycosidase, said method characterized in that it comprises cleaving said linkage with a Type II endoglycosidase to release said distal portion from said proximal portion.
Claim 2
Independent Claim
101 words
A method for releasing at least a portion of a substance, preferably a glycoprotein-containing substance, more preferably a Type II endoglycosidase reactive substance, from a surface to which it is bound other than by an immunological bond, said substance having a proximal portion bound to said surface, a distal portion extending outwardly from said proximal portion, and a linkage disposed at the juncture of said proximal and distal portions which is reactive with a Type II endoglycosidase, said method characterized in that it comprises cleaving said linkage with a Type II endoglycosidase to release said distal portion from said proximal portion.
Claim 3
Independent Claim
84 words
A method for releasing at least a portion of a glycoside-containing substance, preferably a glycoprotein-containing substance, said glycoside-containing substance having at least a carbohydrate portion, an aglycon portion and a glycosidic linkage at the juncture of said carbohydrate and aglycon portions, said substance being bound through said carbohydrate portion to said surface, said method characterized in that it comprises cleaving said glycosidic linkage by reacting it with a Type II endoglycosidase to release the aglycon portion of said glycoside-containing substance from said carbohydrate portion.
Claim 4
Independent Claim
84 words
A method for releasing at least a portion of a glycoside-containing substance, preferably a glycoprotein-containing substance, said glycoside-containing substance having at least a carbohydrate portion, an aglycon portion and a glycosidic linkage at the juncture of said carbohydrate and aglycon portions, said substance being bound through said carbohydrate portion to said surface, said method characterized in that it comprises cleaving said glycosidic linkage by reacting it with a Type II endoglycosidase to release the aglycon portion of said glycoside-containing substance from said carbohydrate portion.
Claim 5
Independent Claim
123 words
A method for releasing at least a portion of a glycoside-containing substance, preferably a glycoprotein-containing substance, having at least one glycosidic linkage which is bound to a surface, said method characterized in that it comprises contacting said bound glycoside-containing substance with at least a first enzyme and a second enzyme, wherein said first enzyme is a Type II endoglycosidase reactive with a first linkage comprising said glycosidic linkage and said second enzyme, preferably a subtilisin or mutant subtilisin, is reactive with a second linkage in said bound glycoside-containing substance other than said first glycosidic linkage to release from said surface the portion of said glycoside-containing substance distal from said surface which is formed by the cleavage of said first and said second enzymes.
Claim 6
Independent Claim
123 words
A method for releasing at least a portion of a glycoside-containing substance, preferably a glycoprotein-containing substance, having at least one glycosidic linkage which is bound to a surface, said method characterized in that it comprises contacting said bound glycoside-containing substance with at least a first enzyme and a second enzyme, wherein said first enzyme is a Type II endoglycosidase reactive with a first linkage comprising said glycosidic linkage and said second enzyme, preferably a subtilisin or mutant subtilisin, is reactive with a second linkage in said bound glycoside-containing substance other than said first glycosidic linkage to release from said surface the portion of said glycoside-containing substance distal from said surface which is formed by the cleavage of said first and said second enzymes.
Claim 7
Independent Claim
54 words
A method for cleaning a surface on which is bound a glycoside-containing substance, preferably a glycoprotein-containing substance, said method characterized in that it comprises contacting said glycoside-containing substance with a Type II endoglycosidase reactive with a glycosidic linkage in said glycoside-containing substance to release at least a part of said substance from said surface.
Claim 8
Independent Claim
54 words
A method for cleaning a surface on which is bound a glycoside-containing substance, preferably a glycoprotein-containing substance, said method characterized in that it comprises contacting said glycoside-containing substance with a Type II endoglycosidase reactive with a glycosidic linkage in said glycoside-containing substance to release at least a part of said substance from said surface.
Claim 9
Independent Claim
75 words
A method for releasing at least a portion of a glycoside-containing substance, preferably a glycoprotein-containing substance, having at least one glycosidic linkage which is bound to a surface, said method characterized in that it comprises contacting said bound glycoside-containing substance with a Type II endoglycosidase and a detergent, said Type II endoglycosidase being reactive with said glycosidic linkage to release from said surface the cleaved portion of said glycoside-containing substance distal from said glycosidic linkage.
Claim 10
Independent Claim
75 words
A method for releasing at least a portion of a glycoside-containing substance, preferably a glycoprotein-containing substance, having at least one glycosidic linkage which is bound to a surface, said method characterized in that it comprises contacting said bound glycoside-containing substance with a Type II endoglycosidase and a detergent, said Type II endoglycosidase being reactive with said glycosidic linkage to release from said surface the cleaved portion of said glycoside-containing substance distal from said glycosidic linkage.
Claim 11
Independent Claim
65 words
A method according to any one of the preceding claims wherein said Type II endoglycosidase is capable of cleaving at least one glycosidic linkage within the first three, preferably within the first two, glycosidic linkages of the proteincarbohydrate unit juncture of a glycoprotein, and more preferably is capable of cleaving at least one glycosidic linkage within the core structure of an N-linked or O-linked glycoprotein.
Claim 12
Independent Claim
47 words
A method according to any one of the preceding claims wherein the portion of said substance released from said surface is contacted with a washing solution or a fluid containing a detergent to remove said portion from said surface in said washing solution or in said fluid.
Claim 13
Independent Claim
50 words
A method according to any one of the preceding claims wherein said Type II endoglycosidase is selected from the group consisting of Endo-β-N-acetylglucosaminidase, Endo-α-N- acetylgalactosaminidase and Endo-β-N-galactosidase, preferably is selected from the group consisting of Endo-D, Endo-H, Endo-L, Endo-C, Endo-CII, Endo-F-Gal type, Endo-F and PNGaseF, and more preferably is Endo-H.
Claim 14
Independent Claim
23 words
A method according to any one of the preceding claims further comprising contacting said substance with a disulfide cleaving reagent or a surfactant.
Claim 15
Dependent Claim
54 words
A cleaning composition comprising a first enzyme and a second enzyme, characterized in that said first enzyme is a Type II endoglycosidase and said second enzyme, preferably a subtilisin or mutant subtilisin, is selected from the group consisting of proteases, lipases, nucleases, glycosidases different from said first enzyme, preferably an exoglycosidase, and combinations thereof.
Claim 16
Dependent Claim
54 words
A cleaning composition comprising a first enzyme and a second enzyme, characterized in that said first enzyme is a Type II endoglycosidase and said second enzyme, preferably a subtilisin or mutant subtilisin, is selected from the group consisting of proteases, lipases, nucleases, glycosidases different from said first enzyme, preferably an exoglycosidase, and combinations thereof.
Claim 17
Dependent Claim
29 words
The composition of Claim 10 further comprising a detergent surfactant, preferably selected from the group consisting of anionic, nonionic, ampholytic, zwitterionic and cationic surfactants, or a disulfide cleaving reagent.
Claim 18
Dependent Claim
16 words
A composition comprising a detergent surfactant characterized in that it additionally comprises a Type II endoglycosidase.
Claim 19
Dependent Claim
16 words
A composition comprising a detergent surfactant characterized in that it additionally comprises a Type II endoglycosidase.
Claim 20
Dependent Claim
48 words
A composition according to Claim 10, 11 or 12 wherein said Type II endoglycosidase is selected from the group consisting of Endo-β-N-acetylglucosaminidase, Endo-α-acetylgalactosaminidase and Endo-β-N-galactosidase, preferably is selected from the group consisting of Endo-D, Endo-H, Endo-L, Endo-C, Endo-CII, Endo-F-Gal type, Endo-F and PNGaseF, and more preferably is Endo-H.
Claim 21
Dependent Claim
34 words
A cleaning composition characterized in that it comprises a Type II endoglycosidase, preferably selected from the group consisting of Endo-H, Endo-F and Endo-D, in an amount effective for removal of glycosidase-containing substances from surfaces.
Claim 22
Dependent Claim
34 words
A cleaning composition characterized in that it comprises a Type II endoglycosidase, preferably selected from the group consisting of Endo-H, Endo-F and Endo-D, in an amount effective for removal of glycosidase-containing substances from surfaces.
Claim 23
Dependent Claim
20 words
A cleaning composition according to Claim 14 having a pH of from 4 to 10, preferably from 5 to 8.
Claim 24
Dependent Claim
14 words
A cleaning composition according to Claim 14 or 15 further comprising a protease enzyme.
Claim 25
Dependent Claim
26 words
A detergent composition according to Claim 14, 15 or 16 further comprising from 1% to 90%, preferably from 5% to 50%, by weight of detergent surfactant.
Claim 26
Dependent Claim
42 words
A laundry detergent composition according to Claim 14, 15, 16 or 17 further comprising from 1% to 75%, preferably from 5% to 40%, by weight of detergent builder, preferably selected from the group consisting of fatty acids, polycarboxylates, polyphosphates, and mixtures thereof.
Claim 27
Dependent Claim
40 words
A liquid laundry detergent composition according to Claim 18 comprising from 10% to 40% by weight of detergent surfactant, from 10% to 20% by weight of detergent builder, and from 20 ppm to 200 ppm of Endo-H, Endo-F or Endo-D.
Claim 28
Independent Claim
61 words
A method for removing glycoside-containing substance 5, preferably Type II endoglycosidase reactive substances, more preferably N-acetylglucosamine-containing substances, from surfaces, said method characterized in that it comprises contacting said substances with a composition according to any of Claims 10-17 in an amount effective for removal of said substances from the surfaces to which they are attached or in which they have embedded.
References Cited (Backward Citations)
No citation records indexed.
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