Antimicrobial method and formulation employing type II endoglycosidase and antimicrobial agent

Claim 2

The antimicrobial composition of Claim 1 wherein said Type II endoglycosidase comprises 1 ppm to 1200 ppm and said antimicrobial agent comprises 2 ppm to 1200 ppm of said composition.

Claim 3

The antimicrobial composition of Claim 1 or 2 wherein said antimicrobial agent is a bacteriocide selected from the group consisting of chlorhexidine, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, Triclocarban, penicillins, tetracycline and Bacitracin.

Claim 4

The antimicrobial composition according to any one of the preceding claims in the form of a mouthwash, dentifrice or denture cleaner comprising from 50 to 350 ppm of chlorhexidine.

Claim 5

The antimicrobial composition according to any one of the preceding claims comprising 5 to 350 ppm of 2,4,4'-trichloro-2'-hydroxydiphenyl ether.

Claim 6

The antimicrobial composition of Claim 1 or 2 wherein said antimicrobial agent is a fungicide selected from the group consisting of Nystatin, Amphotericin B, Benonyl, Captan and Dichloran.

Claim 7

The antimicrobial composition of Claim 6 in the form of a liquid hand soap comprising from 50 to 400 ppm Endo-H, and preferably from 0.5 to 100 ppm Nystatin.

Claim 8

The antimicrobial composition of Claim 1 or 2 wherein said antimicrobial agent is surfactant-stable antimicrobial enzyme, preferably a lysozyme.

Claim 9

The antimicrobial composition of Claim 1, 2, 3, 5, 6 or 8 in the form of personal care or household cleaning products selected from the group consisting of liquid soap, hard surface cleaner, laundry detergent, anti-acne medication, deodorant, shampoo, face cream, mouthwash, dentifrice, and denture cleaner.

Claim 10

The antimicrobial composition of Claim 9 in the form of a hard surface cleaner comprising from 100 to 1000 ppm endo-β-N-acetylglucosaminidase H and from 0.1 to 20 weight % detergent surfactant.

Claim 11

The antimicrobial composition of Claim 9 in the form of a liquid or granular laundry detergent comprising from 2 to 250 ppm Endo-H and from 5 to 60 weight % detergent surfactant.

Claim 12

The antimicrobial method comprising contacting a microorganism, preferably bacteria, fungus or algae, or a surface containing a microorganism with an antimicrobial composition comprising at least two different components, characterized in that said first component comprises a Type II endoglycosidase, preferably selected from the group consisting of Endo-D, Endo-H, Endo-F and PNGaseF, more preferably Endo-H, and said second component comprises an antimicrobial agent, preferably an antibiotic selected from the group consisting of bacteriocides, fungicides and algicides.

Claim 13

The antimicrobial method of Claim 12 wherein said Type II endoglycosidase comprises 1 ppm to 1200 ppm and said antimicrobial agent comprises 2 ppm to 1200 ppm of said composition.

Claim 14

The antimicrobial method of Claim 12 or 13 wherein said antimicrobial agent is a bacteriocide selected from the group consisting of chlorhexidine, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, Triclocarban, penicillins, tetracycline and Bacitracin, preferably chlorhexidine or 2,4,4'-trichloro-2'-hydroxydiphenyl ether, preferably comprising 5 to 350 ppm of the composition.

Claim 15

The antimicrobial method of Claim 12 or 13 wherein said antimicrobial agent is a fungicide selected from the group consisting of Nystatin, Amphotcricin 8, Benonyl , Captan and Dichloran, preferably Nystatin or Amphotericin B.

Claim 16

The antimicrobial method of Claim 12 or 13 wherein said antimicrobial agent is surfactant-stable antimicrobial enzyme, preferably a lysozyme.

Claim 17

The antimicrobial method of Claim 12 or 13 wherein Endo-H and said antimicrobial agent are contained in mouthwash, dentifrice, or denture cleaner, and said antimicrobial agent preferably is chlorhexidine or surfactant-stable antimicrobial enzyme.

Claim 18

The antimicrobial method of Claim 12 or 13 wherein said microorganism is bacteria, preferably selected from the group consisting of Escherichia coli , Strentococcus mutans , Staphylococcus epidermidis , and Staphylococcus aureus , or fungus, preferably Candida albicans .

Claim 19

The antimicrobial method of any one of Claims 12-18 wherein said method kills said microorganism or inhibits the growth of said microorganism.

Claim 20

The antimicrobial method of any one of Claims 12-19 wherein said microorganism is attached to a surface and said method removes said microorganism from said surface.

Claim 21

The antimicrobial method of any one of Claims 12-20 wherein said antimicrobial composition is contained in personal care or household cleaning products, preferably selected from the group consisting of liquid soap, hard surface cleaner, laundry detergent, anti-acne medication, deodorant, shampoo, and face cream.

Claim 22

The antimicrobial method of Claim 21 wherein said personal care product is liquid hand soap containing an antimicrobial composition comprising from 50 to 400 ppm Endo-H and 5 to 100 ppm 2,4,4'-trichloro-2'-hydroxydiphenyl ether.

Claim 23

The antimicrobial method of Claim 21 wherein said household cleaning product is a hard surface cleaner containing an antimicrobial composition comprising from 100 to 1000 ppm Endo-H and from 0.1 to 20 weight % detergent surfactant.

Claim 24

The antimicrobial method of Claim 21 wherein said household cleaning product is a liquid or granular laundry detergent containing an antimicrobial composition comprising from 2 to 250 ppm Endo-H and from 5 to 60 weight % detergent surfactant.

Claim 25

The antimicrobial method of any one of Claims 12-24 wherein said antimicrobial composition is applied periodically so as to prevent further adhesion of said microorganisms to said surface.

Claim 26

The antimicrobial method of any one of Claims 12-25 wherein said contacting is of plant, vegetable or fruit surfaces, preferably of crops selected from the group consisting of corn, citrus, wheat, tobacco, soybeans, tomatoes and strawberries.

Claim 27

An antimicrobial method for killing or inhibiting microorganisms, preferably bacteria, fungi or algae, said method characterized in that it comprises contacting said microorganisms with a first component comprising an antimicrobial-effective concentration of Type II endoglycosidase, preferably selected from the group consisting of Endo-D, Endo-H, Endo-F and PNGase F, more preferably Endo-H, and preferably contacting said microorganisms with a second component comprising an antimicrobial agent.