Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
167 words
A method for determining ligands in a sample, characterized in that said method comprises mixing with the sample: (a) a tracer having the following formula where R is a ligand or analog thereof having at least one common epitope with a ligand to be determined so that the ligand to be determined and the ligand or analog thereof of the tracer are both specifically recognizable by a given antibody, and n is an integer from one to ten, or a biologically acceptable salt of such a tracer, (b) a monoclonal antibody which is capable of specifically recognizing both the ligand to be determined and the tracer, and (c) glycerol added as a part of a solution of the monoclonal antibody in which the glycerol is present in an amount sufficient to increase the stability of the monoclonal antibody in the solution, and then determining the amount of tracer bound to the antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 2
Independent Claim
167 words
A method for determining ligands in a sample, characterized in that said method comprises mixing with the sample: (a) a tracer having the following formula where R is a ligand or analog thereof having at least one common epitope with a ligand to be determined so that the ligand to be determined and the ligand or analog thereof of the tracer are both specifically recognizable by a given antibody, and n is an integer from one to ten, or a biologically acceptable salt of such a tracer, (b) a monoclonal antibody which is capable of specifically recognizing both the ligand to be determined and the tracer, and (c) glycerol added as a part of a solution of the monoclonal antibody in which the glycerol is present in an amount sufficient to increase the stability of the monoclonal antibody in the solution, and then determining the amount of tracer bound to the antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 3
Dependent Claim
29 words
A method as claimed in claim 1 characterized in that the glycerol is present in the monoclonal antibody solution in an amount ranging from about 5% to about 20%.
Claim 4
Dependent Claim
31 words
A method as claimed in claim 1 characterized in that the glycerol constitutes about 10% of the monoclonal antibody solution that is added to the sample subjected to fluorescence polarization techniques.
Claim 5
Dependent Claim
15 words
A method as claimed in claim 1 characterized in that R has the following structure
Claim 6
Dependent Claim
29 words
A method as claimed in claim 4 characterized in that the glycerol is present in the monoclonal antibody solution in an amount ranging from about 5% to about 20%.
Claim 7
Dependent Claim
31 words
A method as claimed in claim 4 characterized in that the glycerol constitutes about 10% of the monoclonal antibody solution that is added to the sample subjected to fluorescence polarization techniques.
Claim 8
Dependent Claim
60 words
A buffered solution of a monoclonal antibody which is capable of specifically recognizing both a ligand to be determined and a tracer having the formula where R is a ligand or analog thereof, and n is an integer from 1 to 10, and an amount of glycerol sufficient to increase significantly the stability of the monoclonal antibody in the solution.
Claim 9
Dependent Claim
60 words
A buffered solution of a monoclonal antibody which is capable of specifically recognizing both a ligand to be determined and a tracer having the formula where R is a ligand or analog thereof, and n is an integer from 1 to 10, and an amount of glycerol sufficient to increase significantly the stability of the monoclonal antibody in the solution.
Claim 10
Dependent Claim
22 words
A solution as claimed in claim 7 characterized in that the glycerol constitutes from about 5% to about 20% of the solution.
Claim 11
Dependent Claim
22 words
A solution as claimed in claim 7 characterized in that the glycerol constitutes from about 8% to about 12% of the solution.
Claim 12
Dependent Claim
18 words
A solution as claimed in claim 7 characterized in that the glycerol constitutes about 10% of the solution.