Patent US2438761A successfully refreshed from Google Patents.
EP0415679A2
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EP
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Blood separation and analyte detection techniques
Assignee: LifeScan Inc
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Filing: 1990-08-24
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Granted: —
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Expires: 2010-08-24
Abstract
Abstract A separation matrix impregnated with a separating agent separates red blood cells or hemoglobin from whole blood. Upon separation, an impregnated test reagent reacts with the separated substantially clear component fluid to form a colored product which enables a visual or meter test for analyte levels in whole blood. Such a test is appropriate for, among other things measuring glucose, cholesterol, or alcohol levels in whole blood samples.
Inventors
- 👤 Ernest J. Kiser
- 👤 Edward G. Rice
- 👤 Michael F. Tomasco
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 11:48
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
86 words
A reagent strip comprising: a matrix, said matrix impregnated with a separating reagent and a test reagent; said matrix having a thickness, and into which is capable of passing a sample of whole blood; said separating reagent capable of separating from said whole blood a substantially clear component fluid containing an analyte; and said test reagent capable of reacting with said analyte in said substantially clear component fluid to vary coloration of said matrix dependent upon the level of said analyte in said whole blood sample.
Claim 2
Dependent Claim
86 words
A reagent strip comprising: a matrix, said matrix impregnated with a separating reagent and a test reagent; said matrix having a thickness, and into which is capable of passing a sample of whole blood; said separating reagent capable of separating from said whole blood a substantially clear component fluid containing an analyte; and said test reagent capable of reacting with said analyte in said substantially clear component fluid to vary coloration of said matrix dependent upon the level of said analyte in said whole blood sample.
Claim 3
Dependent Claim
32 words
The reagent strip of claim 1 wherein said separating reagents are chosen from the group comprising: polyvinyl alcohol, polyvinyl sulfonic acid, polyethylene glycol, polystyrene sulfonic acid, hydroxypropyl cellulose, polyvinylpyrrolidone, and polyacrylic acid.
Claim 4
Dependent Claim
44 words
The reagent strip of claim 1 or claim 2 wherein said test reagents are chosen from the group comprising: 3-methyl-2-benzothiazolinone hydrazone hydrochloride with 3,dimethylaminobenzoic acid or 3, 5 dichloro-2-hydroxybenzene sulfonic acid; 4-aminoantipyrene with 5-oxo-1 (p-sulfophenyl)-2-pyrazoline-3-carboxylic acid, 4-methoxynaphthol or N-(m-tolyl)-diethanolamine; and 2,2′-azino-di (3-ethylenebenzthiazoline) sulfonic acid.
Claim 5
Dependent Claim
34 words
The reagent strip of any one of claims 1 to 3 wherein said matrix has a thickness of between 100 microns and 3000 microns and a porosity of between 0.5 microns and 150 microns.
Claim 6
Dependent Claim
27 words
The reagent strip of any one of claims 1 to 4 wherein said matrix material is chosen from the group comprising: polyesters, polyamides, polyolefins, polysulfones, and cellulosics.
Claim 7
Dependent Claim
50 words
The reagent strip of any one of claims 1 to 5 wherein an unmeasured whole blood sample is placed on a porous disc near said reagent strip matrix and said sample is contacted with said matrix strip, the side of said strip opposite said sample side visible through said strip.
Claim 8
Dependent Claim
41 words
The reagent strip of any of one of claims 1 to 6 wherein said matrix is comprised of a pair of layers, the first said layer embedded with said separator reagents and the second said layer embedded with said test reagents.
Claim 9
Dependent Claim
14 words
The reagent strip of claim 7 wherein said first layer also includes test reagents.
Claim 10
Dependent Claim
17 words
The reagent strip of claim 7 or claim 8 wherein said second layer also includes separating agents.
Claim 11
Dependent Claim
108 words
A reagent strip comprising: a matrix comprised of a polyamide, and containing with a mixture of a reaction agent comprising: 3-methyl-2-benzothiazoline hydrazone hydrochloride; glucose oxidase; horseradish peroxidase; and 3, dimethylaminobenzoic acid; and a separating agent comprising: 25% weight per volume polyvinyl sulfonic acid at pH 4.5 and 1% weight per weight bentonite; said matrix capable of separating whole blood into red blood cells and a substantially clear fluid through reaction with said separating agent, said reaction reagent reacting with analyte in said substantially clear fluid to color said matrix, said color intensity varying with the level of glucose in said sample; said matrix attached to said reagent strip.
Claim 12
Dependent Claim
108 words
A reagent strip comprising: a matrix comprised of a polyamide, and containing with a mixture of a reaction agent comprising: 3-methyl-2-benzothiazoline hydrazone hydrochloride; glucose oxidase; horseradish peroxidase; and 3, dimethylaminobenzoic acid; and a separating agent comprising: 25% weight per volume polyvinyl sulfonic acid at pH 4.5 and 1% weight per weight bentonite; said matrix capable of separating whole blood into red blood cells and a substantially clear fluid through reaction with said separating agent, said reaction reagent reacting with analyte in said substantially clear fluid to color said matrix, said color intensity varying with the level of glucose in said sample; said matrix attached to said reagent strip.
Claim 13
Independent Claim
73 words
A method for testing analyte levels on a reagent strip comprising: placing a sample of whole blood on said strip; reacting said whole blood with separating reagents impregnated on said strip; reacting said separated sample with test reagents on said strip said test reagents causing said separated sample to vary color of said matrix dependent on the level of analyte in said sample; and measuring said color levels visually or by meter means.
Claim 14
Independent Claim
73 words
A method for testing analyte levels on a reagent strip comprising: placing a sample of whole blood on said strip; reacting said whole blood with separating reagents impregnated on said strip; reacting said separated sample with test reagents on said strip said test reagents causing said separated sample to vary color of said matrix dependent on the level of analyte in said sample; and measuring said color levels visually or by meter means.
References Cited (Backward Citations)
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