EP0413440A2
Expired / Lapsed
EP
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Peptidyl prolyl-cis.trans-isomerase
Assignee: Tonen General Sekiyu KK
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Filing: 1990-07-19
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Granted: —
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Expires: 2010-07-19
Abstract
Abstract A yeast PPIase characterized by possessing the following properties: (1) acting on and isomerizing the bond X aa -Pro (wherein X aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA; an E . coli PPIase-β characterized by possessing the following properties: (1) acting on and isomerizing the bond X aa -Pro (wherein X aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA; an E . coli PPIase-α characterized by possessing the following properties: (1) acting on and isomerizing the bond X aa -Pro (wherein X aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.
Inventors
- 👤 Toshiya C/O Tonen Corporation Corp. Res. Hayano
- 👤 Setsuko C/O Tonen Corporation Corp. Res. Katou
- 👤 Noboru C/O Tonen Corporation Corp. Res. Maki
- 👤 Nobuhiro Tonen Corporation Corp. Res. Takahashi
- 👤 Masanori C/O Tonen Corporation Corp. Res. Suzuki
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 13:00
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/90
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
70 words
A yeast peptidyl-prolyl cis-trans isomerase possessing the characteristics: (1) acting on and isomerizing an X aa -Pro bond (wherein X aa stands for any amino acid and Pro for L-proline) in a peptide chain, (2) exhibiting a single molecular weight of about 17,000 daltons on sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.2 on isoelectric focusing, and (4) being inhibited by CsA.
Claim 2
Dependent Claim
70 words
A yeast peptidyl-prolyl cis-trans isomerase possessing the characteristics: (1) acting on and isomerizing an X aa -Pro bond (wherein X aa stands for any amino acid and Pro for L-proline) in a peptide chain, (2) exhibiting a single molecular weight of about 17,000 daltons on sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.2 on isoelectric focusing, and (4) being inhibited by CsA.
Claim 3
Dependent Claim
175 words
An enzyme according to claim 1, which possesses the following amino acid sequence: (Met) Ser Gln Val Tyr Phe Asp Val Glu Ala Asp Gly Gln Pro Ile Gly Arg Val Val Phe Lys Leu Tyr Asn Asp Ile Val Pro Lys Thr Ala Glu Asn Phe Arg Ala Leu Cys Thr Gly Glu Lys Gly Phe Gly Tyr Ala Gly Ser Pro Phe His Arg Val Ile Pro Asp Phe Met Leu Gln Gly Gly Asp Phe Thr Ala Gly Asn Gly Thr Gly Gly Lys Ser Ile Tyr Gly Gly Lys Phe Pro Asp Glu Asn Phe Lys Lys His His Asp Arg Pro Gly Leu Leu Ser Met Ala Asn Ala Gly Pro Asn Thr Asn Gly Ser Gln Phe Phe Ile Thr Thr Val Pro Cys Pro Trp Leu Asp Gly Lys His Val Val Phe Gly Glu Val Val Asp Gly Tyr Asp Ile Val Lys Lys Val Glu Ser Leu Gly Ser Pro Ser Gly Ala Thr Lys Ala Arg Ile Val Val Ala Lys Ser Gly Glu Leu.
Claim 4
Dependent Claim
26 words
A method of producing of yeast peptidyl-prolyl cis-trans isomerase, characterized by recovering the enzyme of claim 1 or claim 2 from yeast cells of Saccharomyces .
Claim 5
Dependent Claim
14 words
A gene coding for yeast peptidyl-prolyl cis-trans isomerase of claim 1 or claim 2.
Claim 6
Dependent Claim
174 words
A gene according to claim 4, which possesses the following base sequence: ATG TCC CAA GTC TAT TTT GAT GTC GAA GCT GAT GGC CAA CCA ATT GGC CGT GTC GTT TTC AAG TTG TAC AAC GAC ATA GTC CCA AAG ACT GCA GAA AAC TTC AGA GCT CTA TGT ACC GGT GAS AAG GGA TTC GGC TAC GCT GGC TCT CCA TTC CAC AGA GTT ATT CCA GAC TTC ATG TTG CAA GGT GGT GAC TTC ACT GCT GGT AAC GGT ACC GGC GGT AAG TCT ATC TAC GGT GGC AAA TTC CCA GAT GAA AAC TTC AAG AAG CAC CAC GAC AGA CCA GGT TTG TTG TCC ATG GCC AAC GCC GGT CCA AAC ACC AAC GGT TCT CAA TTC TTC ATC ACC ACC GTT CCA TGC CCA TGG TTG GAC GGT AAG CAT GTT GTC TTT GGT GAA GTT GTT GAC GGT TAC GAC ATC GTT AAG AAG GTT GAG TCC TTG GGT TCT CCT TCC GGT GCC ACC AAG GCT AGA ATT GTT GTT GCC AAG TCC GGT GAA TTA.
Claim 7
Dependent Claim
74 words
An Escherichia coli peptidyl prolyl cis·trans isomerase β possessing the characteristics: (1) acting on and isomerizing an X aa -Pro bond (wherein X aa stands for any amino acid and Pro for L-proline) in a peptide chain, (2) exhibiting a single molecular weight of about 20,000 daltons on sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 on isoelectric focusing, and (4) not being inhibited by CsA.
Claim 8
Dependent Claim
74 words
An Escherichia coli peptidyl prolyl cis·trans isomerase β possessing the characteristics: (1) acting on and isomerizing an X aa -Pro bond (wherein X aa stands for any amino acid and Pro for L-proline) in a peptide chain, (2) exhibiting a single molecular weight of about 20,000 daltons on sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 on isoelectric focusing, and (4) not being inhibited by CsA.
Claim 9
Dependent Claim
178 words
The enzyme β according to claim 6, which possesses the following amino acid sequence: Met Val Thr Phe His Thr Asn His Gly Asp Ile Val Ile Lys Thr Phe Asp Asp Lys Ala Pro Glu Thr Val Lys Asn Phe Leu Asp Tyr Cys Arg Glu Gly Phe Tyr Asn Asn Thr Ile Phe His Arg Val Ile Asn Gly Phe Met Ile Gln Gly Gly Gly Phe Glu Pro Gly Met Lys Gln Lys Ala Thr Lys Glu Pro Ile Lys Asn Glu Ala Asn Asn Gly Leu Lys Asn Thr Arg Gly Thr Leu Ala Met Ala Arg Thr Gln Ala Pro His Ser Ala Thr Ala Gln Phe Phe Ile Ans Val Val Asp Asn Asp Phe Leu Asn Phe Ser Gly Glu Ser Leu Gln Gly Trp Gly Tyr Cys Val Phe Ala Glu Val Val Asp Gly Met Asp Glu Val Asp Lys Ile Lys Gly Val Ala Thr Gly Arg Ser Gly Met His Gln Asp Val Pro Lys Glu Asp Val Ile Ile Glu Ser Val Thr Val Ser Glu.
Claim 10
Dependent Claim
29 words
A method for producing of an Escherichia coli peptidyl-prolyl cis-trans isomerase β, characterized by recovering the enzyme of claim 6 or claim 7 from cells of Escherichia coli .
Claim 11
Dependent Claim
17 words
A gene coding for an Escherichia coli peptidyl-prolyl cis-trans isomerase β of claim 6 or claim 7.
Claim 12
Dependent Claim
176 words
A gene according to claim 9, which possesses the following base sequence: ATG GTT ACT TTC CAC ACC AAT CAC GGC GAT ATT GTC ATC AAA ACT TTT GAC GAT AAA GCA CCT GAA ACA GTT AAA AAC TTC CTG GAC TAC TGC CGC GAA GGT TTT TAC AAC AAC ACC ATT TTC CAC CGT GTT ATC AAC GGC TTT ATG ATT CAG GGC GGC GGT TTT GAA CCG GGC ATG AAA CAA AAA GCC ACC AAA GAA CCG ATC AAA AAC GAA GCC AAC AAC GGC CTG AAA AAT ACC CGT GGT ACG CTG GCA ATG GCA CGT ACT CAG GCT CCG CAC TCT GCA ACT GCA CAG TTC TTC ATC AAC GTG GTT GAT AAC GAC TTC CTG AAC TTC TCT GGC GAA AGC CTG CAA GGT TGG GGC TAC TGC GTG TTT GCT GAA GTG GTT GAC GGC ATG GAC GAG GTA GAC AAA ATC AAA GGT GTT GCA ACC GGT CGT AGC GGT ATG CAC CAG GAC GTG CCA AAA GAA GAC GTT ATC ATT GAA AGC GTG ACC GTT AGC GAG.
Claim 13
Dependent Claim
12 words
An expression plasmid containing a gene of claim 9 or claim 10.
Claim 14
Dependent Claim
39 words
A method of producing of an Escherichia coli peptidyl-prolyl cis-trans isomerase β, characterized by culturing a host microorganism transformed with an expression plasmid of claim 11 and recovering an Escherichia coli peptidyl-prolyl cis-trans isomerase β from the resultant culture.
Claim 15
Dependent Claim
74 words
An Escherichia coli peptidyl-prolyl·cis-trans isomerase α possessing the characteristics: (1) acting on and isomerizing an X aa -Pro bond (wherein X aa stands for any amino acid and Pro for L-proline) in a peptide chain, (2) exhibiting a single molecular weight of about 22,000 daltons on the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 on the isoelectric focusing, and (4) not being inhibited by CsA.
Claim 16
Dependent Claim
74 words
An Escherichia coli peptidyl-prolyl·cis-trans isomerase α possessing the characteristics: (1) acting on and isomerizing an X aa -Pro bond (wherein X aa stands for any amino acid and Pro for L-proline) in a peptide chain, (2) exhibiting a single molecular weight of about 22,000 daltons on the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 on the isoelectric focusing, and (4) not being inhibited by CsA.
Claim 17
Dependent Claim
20 words
An Escherichia coli peptidyl-prolyl cis-trans isomerase α according to claim 13, which possesses the following partial amino acid sequence: Ala-Lys-Gly-Asp-Pro-His-Val-Leu-Leu-Thr-Thr-Ala-Gly-Val-Asn-Ile-Glu-Leu-X-Leu-Asp-Lys-X-Lys.
Claim 18
Dependent Claim
25 words
A method of producing an Escherichia coli peptidyl-prolyl cis-trans isomerase α, characterized by recovering an enzyme of claim 13 from cells of Escherichia coli .
References Cited (Backward Citations)
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