EP0411925A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Process for cross-linking collagenous materials and resulting product
Assignee: University of North Carolina at Chapel Hill
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Filing: 1990-08-01
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Granted: —
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Expires: 2010-08-01
Abstract
Abstract The present invention relates to a process for cross-linking a proteinaceous material. The process comprises: i) soaking the material to be cross-linked in an agueous solution of high osmolality; ii) incubating the material in an agueous buffer including an amount of a photooxidative catalyst sufficient to catalyze photooxidation of the material; and iii) irradiating the material and the catalyst of step (i) with light that includes a range of wavelengths selectively absorbed by the catalyst. Irradiation is effected under conditions such that cross-linking of the material occurs. In a further embodiment, the present invention relates to a cross-linked product produced by the above-described method.
Inventors
- 👤 Gerald L. Mechanic
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 15:13
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3687; C; C08; C08H; C08H1/00; C08H1/06
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
145 words
A process for cross-linking proteinaceous materials comprising: incubating a sample of a proteinaceous material to be cross-linked in an aqueous media solution containing a photooxidative catalyst capable of donating electrons to the proteinaceous material when excited by incident light, the media solution being buffered to a pH in the range of from 6.8 to 8.6, for a period of time sufficient to allow equilibration of the concentrations of media solution, proteinaceous material, and catalyst and irradiating the proteinaceous material with light in the presence of oxygen for a period of time sufficient to cross-link the proteinaceous material by transfer of electrons from the catalyst to the proteinaceous material while maintaining the pH of the media solution in the range of from 6.8 to 8.6 and holding the temperature in the range of from -2 to 40°C to maintain the oxygen concentration in the media solution.
Claim 2
Dependent Claim
145 words
A process for cross-linking proteinaceous materials comprising: incubating a sample of a proteinaceous material to be cross-linked in an aqueous media solution containing a photooxidative catalyst capable of donating electrons to the proteinaceous material when excited by incident light, the media solution being buffered to a pH in the range of from 6.8 to 8.6, for a period of time sufficient to allow equilibration of the concentrations of media solution, proteinaceous material, and catalyst and irradiating the proteinaceous material with light in the presence of oxygen for a period of time sufficient to cross-link the proteinaceous material by transfer of electrons from the catalyst to the proteinaceous material while maintaining the pH of the media solution in the range of from 6.8 to 8.6 and holding the temperature in the range of from -2 to 40°C to maintain the oxygen concentration in the media solution.
Claim 3
Dependent Claim
19 words
A process according to Claim 1, wherein the pH is maintained in a range of from 7.4 to 8.0.
Claim 4
Dependent Claim
37 words
A process according to Claim 1 or 2, wherein the oxygen concentration in the media is maintained during irradiation by maintaining the oxygen concentration of the atmosphere above the media at greater than 0 up to 25%.
Claim 5
Dependent Claim
33 words
A process according to Claim 3, wherein the oxygen concentration of the media during irradiation is maintained by maintaining the oxygen concentration of the atmosphere above the media at from 5 to 20%.
Claim 6
Dependent Claim
23 words
A process according to any one of the preceding claims, wherein the proteinaceous material is irradiated for from 100 to 20,000 lumen hours.
Claim 7
Dependent Claim
29 words
A process according to any one of the preceding claims, wherein the proteinaceous material is soaked in an aqueous buffer solution before being incubated in the aqueous media solution.
Claim 8
Dependent Claim
23 words
A process according to Claim 6, wherein the osmolality of the buffer solution is in the range of from 393 to 800 mosm.
Claim 9
Dependent Claim
23 words
A process according to Claim 7, wherein the osmolality of the media solution is in the range of from 150 to 400 mosm.
Claim 10
Dependent Claim
23 words
A process according to any one of the preceding claims, wherein the temperature is maintained in the range of from 0 to 25°C.
Claim 11
Dependent Claim
76 words
A cross-linked product producible by the method of: soaking collagen molecules in an aqueous medium; equilibrating the collagen molecules with a photooxidative catalyst; and thereafter oxidizing the collagen molecules to form cross-linkages therebetween by inducing the transfer of electrons from the photooxidative catalyst to the collagen molecules in the presence of oxygen by exposing the collagen molecules to light while holding temperature and pH at levels sufficient to maintain the oxygen concentration in the aqueous medium.
Claim 12
Dependent Claim
76 words
A cross-linked product producible by the method of: soaking collagen molecules in an aqueous medium; equilibrating the collagen molecules with a photooxidative catalyst; and thereafter oxidizing the collagen molecules to form cross-linkages therebetween by inducing the transfer of electrons from the photooxidative catalyst to the collagen molecules in the presence of oxygen by exposing the collagen molecules to light while holding temperature and pH at levels sufficient to maintain the oxygen concentration in the aqueous medium.
Claim 13
Dependent Claim
22 words
A product according to Claim 10, wherein the medium is buffered to a pH in the range of from 6.8 to 8.6.
Claim 14
Dependent Claim
23 words
A product according to Claim 10 or 11, wherein the concentration of catalyst is in the range of from 0.0001% to 0.25% w/v.
Claim 15
Dependent Claim
26 words
A product according to Claim 10, 11 or 12, wherein the collagen molecules are photooxidized at a temperature in the range of from -2°C to +40°C.
Claim 16
Dependent Claim
24 words
A product according to any one of Claims 10 to 13, wherein the collagen molecules are irradiated for from 100 to 20,000 lumen hours.
Claim 17
Dependent Claim
28 words
A product according to any one of Claims 10 to 14, wherein the osmolality of the aqueous medium is in the range of from 150 to 800 mosm.
Claim 18
Dependent Claim
20 words
A product according to any one of Claims 10 to 15, wherein the catalyst is dissolved in the aqueous buffer.
References Cited (Backward Citations)
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