EP0397269A1
Expired / Lapsed
EP
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Method for the synthesis of ribonucleic acid (RNA)
Assignee: Akzo Nobel NV
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Filing: 1990-05-08
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Granted: —
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Expires: 2010-05-08
Abstract
Abstract The invention relates to a method for the synthesis of ribonucleic acid starting from deoxyribonucleic acid in which a target sequence is present. Said DNA is treated with restriction enzyme(s) followed by strand separation. Subsequently one uses one nucleic acid primer containing a promoter sequence coupled to a nucleotide sequence which corresponds to a part of the target sequence. The invention further relates to a test kit for synthesizing RNA.
Inventors
- 👤 Lawrence T. Malek
- 👤 Cheryl Davey
- 👤 Peter Franklin Lens
- 👤 Frits Wielaard
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 08:44
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6865; C07; C07H; C07H21/00; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12Q1/70; C12Q1/701; C12Q1/705
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
122 words
Method for the synthesis of ribonucleic acid (RNA) starting from deoxyribonucleic acid (DNA) in which a target sequence is present, by treating this DNA with one or more restriction enzymes, followed by a separation of the DNA fragment thus obtained, by which means single-strand DNA is formed, after which one nucleic acid primer containing a promoter sequence which is coupled to a nucleotide sequence which corresponds to a part of the target sequence is hybridized under suitable conditions on the corresponding single-strand DNA, after which the two nucleic acid sequences obtained in hybrid form are extended from the free 3′-end by a DNA polymerase to produce a double-strand DNA, which is then used as a matrix by RNA polymerase for synthesizing RNA.
Claim 2
Dependent Claim
122 words
Method for the synthesis of ribonucleic acid (RNA) starting from deoxyribonucleic acid (DNA) in which a target sequence is present, by treating this DNA with one or more restriction enzymes, followed by a separation of the DNA fragment thus obtained, by which means single-strand DNA is formed, after which one nucleic acid primer containing a promoter sequence which is coupled to a nucleotide sequence which corresponds to a part of the target sequence is hybridized under suitable conditions on the corresponding single-strand DNA, after which the two nucleic acid sequences obtained in hybrid form are extended from the free 3′-end by a DNA polymerase to produce a double-strand DNA, which is then used as a matrix by RNA polymerase for synthesizing RNA.
Claim 3
Dependent Claim
35 words
Method according to Claim 1, characterized in that the synthesized RNA is detected by means of gel electrophoresis under denaturing conditions followed by blotting, after which hybridization with a labelled specific oligonucleotide sequence takes place.
Claim 4
Dependent Claim
9 words
Test kit for synthesizing RNA according to Claim 1.
References Cited (Backward Citations)
WO1988010315A1
Cited
WO1989006700A1
Cited
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