EP0391608A2
Expired / Lapsed
EP
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Metal oxide supports for nucleic acids
Assignee: 3M Co
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Filing: 1990-03-29
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Granted: —
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Expires: 2010-03-29
Abstract
Abstract Nucleic acids sorbed to metal oxide supports with sufficient strength and in sufficient amounts to enable the resulting compositions of matter to be used for such purposes as hybridizing, labeling, sequencing, and synthesis of nucleic acids. The compositions can optionally also be bound with blocking agent to prevent undesired nucleic acid sorption, e.g., of probe nucleic acids in a hybridization experiment. Preferred compositions are easily prepared and used, versatile, and reusable.
Inventors
- 👤 Rex M. C/O Minnesota Mining And Bitner
- 👤 Eric F. C/O Minnesota Mining And Funkenbusch
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 05:02
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12N11/00; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/553
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
52 words
A composition of matter comprising; (a) a support comprising a sufficient amount of metal oxide at its surface to sorb nucleic acid, (b) nucleic acid sorbed to at least a portion of the available surface of said support in a manner such that said nucleic acid substantially retains biological accessibility and reactivity.
Claim 2
Dependent Claim
52 words
A composition of matter comprising; (a) a support comprising a sufficient amount of metal oxide at its surface to sorb nucleic acid, (b) nucleic acid sorbed to at least a portion of the available surface of said support in a manner such that said nucleic acid substantially retains biological accessibility and reactivity.
Claim 3
Dependent Claim
62 words
A composition according to claim 1 further comprising blocking agent bound to substantially the remainder of the available surface of said support in a manner that substantially precludes the ability of the support to sorb additional nucleic acid, wherein said blocking agent is selected from the group consisting of organic or inorganic molecules providing phosphate, phosphonate, pyrophosphate, carboxylate, sulfate and fluoride groups.
Claim 4
Dependent Claim
50 words
A composition according to claim 1 wherein said support is in a form selected from the group consisting of particles, fibers, and monolithic bodies, and wherein said metal oxide is selected from the group consisting of magnesium, aluminum, calcium, titanium, manganese, iron, cobalt, nickel, zinc, yttrium, zirconium, lanthanum, and hafnium.
Claim 5
Dependent Claim
34 words
A composition according to claim 3 wherein said support is in the form of a particle and said particle is used in a conformation selected from the group consisting of suspensions, coatings, and composites.
Claim 6
Dependent Claim
21 words
A composition according to claim 4 wherein said conformation is a composite provided in the form of a web of PTFE.
Claim 7
Dependent Claim
14 words
A composition according to claim 3 wherein said metal oxide is zirconium or aluminum.
Claim 8
Independent Claim
61 words
A method of preparing a composition of matter comprising the steps of: (a) providing a support comprising an amount of metal oxide at its surface sufficient to sorb nucleic acid, and (b) sorbing nucleic acid to at least a portion of the available surface of said support in a manner that substantially retains biological accessibility and reactivity of the nucleic acid.
Claim 9
Independent Claim
61 words
A method of preparing a composition of matter comprising the steps of: (a) providing a support comprising an amount of metal oxide at its surface sufficient to sorb nucleic acid, and (b) sorbing nucleic acid to at least a portion of the available surface of said support in a manner that substantially retains biological accessibility and reactivity of the nucleic acid.
Claim 10
Dependent Claim
41 words
A method according to claim 16 further comprising the step of binding blocking agent to substantially the remainder of the available surface of said support in a manner that substantially precludes the ability of the support to sorb additional nucleic acid.
Claim 11
Independent Claim
104 words
A method of hybridizing, labeling, and/or sequencing nucleic acid comprising the steps of (a) providing a composition comprising (i) a support comprising an amount of metal oxide sufficient to sorb nucleic acid, (ii) nucleic acid sorbed to at least a portion of the available surface of said support in a manner such that said nucleic acid substantially retains biological accessibility and reactivity, and (iii) blocking agent bound to substantially the remainder of the available surface of said support in a manner that substantially precludes the ability of the support to sorb additional nucleic acid, and (b) hybridizing, labeling, or sequencing said sorbed nucleic acid.
Claim 12
Independent Claim
104 words
A method of hybridizing, labeling, and/or sequencing nucleic acid comprising the steps of (a) providing a composition comprising (i) a support comprising an amount of metal oxide sufficient to sorb nucleic acid, (ii) nucleic acid sorbed to at least a portion of the available surface of said support in a manner such that said nucleic acid substantially retains biological accessibility and reactivity, and (iii) blocking agent bound to substantially the remainder of the available surface of said support in a manner that substantially precludes the ability of the support to sorb additional nucleic acid, and (b) hybridizing, labeling, or sequencing said sorbed nucleic acid.
References Cited (Backward Citations)
No citation records indexed.
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