Patent EP0496009A1 successfully refreshed from Google Patents.
EP0388152A2
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Process for producing an immunosuppressant agent (demethimmunomycin) using a mutant strain of a microorganism
Assignee: Merck and Co Inc
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Filing: 1990-03-13
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Granted: —
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Expires: 2010-03-13
Abstract
Abstract Described is a new process for producing the immunosuppressant, L-683,742, a C-31 demethylated, derivative of L-683,590 (FK-520), produced under fermentation conditions utilizing the new mutant microorganism, Streptomyces hygroscopicus subsp. ascomyceticus (Merck Culture Collection MA 6646) ATCC No. 53855, being a blocked mutant of Streptomyces hygroscopicus subsp. ascomyceticus (MA 6475) ATCC No. 14891. The macrolide immunosuppressant is useful in preventing human host rejection of foreign organ transplants, e.g. bone marrow and heart transplants.
Inventors
- 👤 Carolyn L. Ruby
- 👤 Byron H. Arison
- 👤 Susan J. Danis
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 05:21
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/01; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P17/00; C12P17/18; C12P17/188; C12R; C12R2001/00; C12R2001/01; C12R2001/465; C12R2001/55
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
62 words
A process for producing an immunosuppressant, identified as a C-31 demethylated derivative of a L-683,590 type macrolide comprising the step of culturing a mutant of a Streptomyces which produces said C-31 demethylated derivative under submerged aerobic fermentation conditions in an aqueous carbohydrate medium containing a nitrogen nutrient at a pH below 8.0 for a sufficient time to produce the C-31 demethylated immunosuppressant.
Claim 2
Dependent Claim
62 words
A process for producing an immunosuppressant, identified as a C-31 demethylated derivative of a L-683,590 type macrolide comprising the step of culturing a mutant of a Streptomyces which produces said C-31 demethylated derivative under submerged aerobic fermentation conditions in an aqueous carbohydrate medium containing a nitrogen nutrient at a pH below 8.0 for a sufficient time to produce the C-31 demethylated immunosuppressant.
Claim 3
Dependent Claim
49 words
A process for producing an immunosuppressant, identified as L-683,742, comprising the step of culturing a strain of mutant "L-683,742-producing" Streptomyces hygroscopicus var. ascomyceticus under submerged aerobic fermentation conditions in an aqueous carbohydrate medium containing a nitrogen nutrient at a pH below 8.0 for a sufficient time to produce L-683,742.
Claim 4
Dependent Claim
49 words
A process for producing an immunosuppressant, identified as L-683,742, comprising the step of culturing a strain of mutant "L-683,742-producing" Streptomyces hygroscopicus var. ascomyceticus under submerged aerobic fermentation conditions in an aqueous carbohydrate medium containing a nitrogen nutrient at a pH below 8.0 for a sufficient time to produce L-683,742.
Claim 5
Dependent Claim
14 words
The process of Claim 2 wherein said mutant microorganism is ATCC No. 53855 .
Claim 6
Dependent Claim
15 words
The broth produced by the process of Claim 2 exhibiting positive inhibition of T-cell activation.
Claim 7
Dependent Claim
12 words
A mutant microorganism Streptomyces hygroscopicus subsp. ascomyceticus , ATCC No. 53855 .
Claim 8
Dependent Claim
12 words
A mutant microorganism Streptomyces hygroscopicus subsp. ascomyceticus , ATCC No. 53855 .
Claim 9
Dependent Claim
10 words
The mutant microorganism of claim 5 in biologically pure form.
References Cited (Backward Citations)
No citation records indexed.
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