EP0385433A2
Expired / Lapsed
EP
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Method and apparatus for the continuous polymer synthesis on a solid carrier
Assignee: Czech Academy of Sciences
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Filing: 1990-02-28
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Granted: —
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Expires: 2010-02-28
Abstract
Abstract The invention relates to a novel method and apparatus for the solid-phase synthesis of polymers such as polypeptides wherein an elongated, preferably band-like carrier (1) is used. The attachment of the reactants is realized by successive introduction of the carrier (1) into the appropriate baths. This method allows continuous preparation of polypeptides, polynucleotides, etc. The apparatus comprises a solid, insoluble, elongated carrier (1) comprising functional groups and an arrangement of successive reaction zones (5, 7) and processing zones (4, 6, 8) of a sequence identical with that of the reaction and processing steps of the process sequence of the respective synthesis, and comprises means (2a, 2b) for successively contacting the carrier (1) with the reaction and processing liquids.
Inventors
- 👤 Michal Lébl
- 👤 Vladimir Gut
- 👤 Jutta Eichler
- 👤 Viktor Krchnák
- 👤 Josef Vágner
- 👤 Jiri Stepánek
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 18:26
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/04; C07K1/045; B; B01; B01J; B01J19/00; B01J19/0046; B01J19/18; B01J19/22; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00497; B01J2219/00513; B01J2219/00515; B01J2219/00518; B01J2219/00527; B01J2219/00533; B01J2219/00583; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00623; B01J2219/00626; B01J2219/00657; B01J2219/00659; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B40/10
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
100 words
A method for the continuous synthesis of oligomeric and polymeric organic compounds, particularly of biopolymers and preferably of polypeptides and polynucleotides, characterized in that - a solid, insoluble, elongated carrier is used comprising functional groups serving for anchoring the first reaction component of the process sequence of the respective synthesis either directly or by means of a spacer, and - the individual reaction and processing steps are carried out in successive zones of a sequence identical with that of the reaction and processing steps of the process sequence moving in one direction given by the longest dimension of the carrier.
Claim 2
Independent Claim
100 words
A method for the continuous synthesis of oligomeric and polymeric organic compounds, particularly of biopolymers and preferably of polypeptides and polynucleotides, characterized in that - a solid, insoluble, elongated carrier is used comprising functional groups serving for anchoring the first reaction component of the process sequence of the respective synthesis either directly or by means of a spacer, and - the individual reaction and processing steps are carried out in successive zones of a sequence identical with that of the reaction and processing steps of the process sequence moving in one direction given by the longest dimension of the carrier.
Claim 3
Dependent Claim
31 words
The method according to claim 1, characterized in that all reaction and processing steps of the process sequence are carried out at the same time in different parts of the carrier.
Claim 4
Independent Claim
26 words
The method according to claims 1 and 2, characterized in that the carrier is successively subjected to the reaction and processing steps of the process sequence.
Claim 5
Independent Claim
25 words
The method according to claims 1 to 3, characterized in that the carrier is moved through the successive zones, preferably with a predetermined, constant speed.
Claim 6
Independent Claim
19 words
The method according to claims 1 to 4, characterized in that a planar, band-shaped, or thread-like carrier is used.
Claim 7
Independent Claim
44 words
The method according to claims 1 to 5, characterized in that a carrier is used made of a cellulose material, such as cotton fabric or paper, modified polystyrene, polytetrafluoroethylene, polyamide, modified polypropylene, or functionalized glass, such as modified glass fibers or modified fibre glass.
Claim 8
Independent Claim
46 words
The method according to claims 1 to 6, characterized in that an open- or closed-loop carrier is used the length of which at least corresponds to the total residence time necessary for all reaction and processing steps of the process sequence for a given moving speed.
Claim 9
Independent Claim
76 words
The method according to claims 1 to 7, characterized in that the synthesis process is stopped at a stage where a series of intermediate compounds and eventually also the derived final product corresponding to the respective series of reaction steps of the process sequence have been formed in the corresponding parts of the carrier, and these compounds are removed from the carrier either continuously or in a batch-like manner from the respective parts of the carrier.
Claim 10
Independent Claim
37 words
The method according to claims 1 to 8, characterized in that the final product is removed from the carrier after completion of the total process sequence in all parts of the carrier, preferably in a batch-like manner.
Claim 11
Independent Claim
106 words
An apparatus for the continuous synthesis of oligomeric and polymeric organic compounds, particularly for carrying out the method according to claims 1 to 9, comprising - a solid, insoluble, elongated carrier (1) comprising functional groups serving for anchoring the first reaction component of the process sequence, and - an arrangement of successive reaction and processing zones (4, 5, 6, 7, 8) of a sequence identical with that of the reaction and processing steps of the process sequence of the respective synthesis, and comprising means (2a, 2b) for successively contacting the carrier (1) with the liquids of the reaction and processing zones (4, 5, 6, 7, 8).
Claim 12
Dependent Claim
30 words
The apparatus according to claim 10, comprising means for continuously moving the carrier (1) through the reaction and processing zones (4, 5, 6, 7, 8) with a predetermined, constant speed.
Claim 13
Independent Claim
77 words
The apparatus according to claims 10 and 11, characterized in that it comprises an open- or closed-loop carrier (1) the length of which at least corresponds to the total residence time necessary for all reaction and processing steps of the process sequence for a given moving speed, and the carrier path length of the individual reaction and processing steps corresponds to the residence time necessary for the respective reaction or processing step for the given moving speed.
Claim 14
Independent Claim
19 words
The apparatus according to claims 10 to 12, characterized in that the carrier (1) is planar, band-shaped or thread-like.
Claim 15
Dependent Claim
56 words
Carrier for use in the method according to claims 1 to 9 and/or in the apparatus according to claims 10 to 13, characterized in that it is made of a cellulose material, such as cotton fabric or paper, modified polystyrene, polytetrafluoroethylene, polyamide, modified polypropylene, or functionalized glass, such as modified glass fibers or modified fibre glass.
Claim 16
Dependent Claim
35 words
Use of the apparatus according to claims 10 to 13 and/or of the carrier according to claim 14 for and/or application of the method according to claims 1 to 9 to peptide or polynucleotide synthesis.
References Cited (Backward Citations)
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