EP0375438A2
Expired / Lapsed
EP
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Recombinant DNA molecules, hosts, processes and human somatomedin carrier protein-like polypeptides
Assignee: Celtrix Pharmaceuticals Inc
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Filing: 1989-12-21
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Granted: —
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Expires: 2009-12-21
Abstract
Abstract This invention relates to polypeptides that are human somatomedin carrier protein subunits and to processes for producing them. The carrier protein subunits bind to human somatomedin-like polypeptides, also known as insulin-like growth factors. The process involves preparation from a human serum fraction, Cohn IV-1, by a molecule of various chromatographic steps. This invention also relates to DNA molecules encoding human somatomedin carrier protein-like polypeptides, recombinant DNA molecules, hosts, processes for producing carrier protein-like polypeptides, human somatomedin carrier protein-like polypeptides produced using those molecules, hosts and processes. The invention relates to DNA molecules and their expression in appropriate hosts. The recombinant DNA molecules contain DNA molecules that code for polypeptides which have a biological activity of the human carrier protein or a human carrier protein subunit capable of binding somatomedins. The DNA molecules, recombinant DNA molecules, hosts, and processes of this invention may be used in the production of polypeptides useful in a variety of therapeutic, diagnostic, and other useful applications.
Inventors
- 👤 Emerald Martin Spencer
- 👤 Carol Talkington-Verser
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 13:14
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; C07K14/46; C07K14/47; C07K14/4701; C07K14/4743; A; A61; A61P; A61P19/00; A61P19/08; A61P19/10; A61P27/00; A61P27/02; A61P3/00; A61P3/02; A61P43/00; A61P5/00; A61P5/06; A61K; A61K38/00
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
13 words
A DNA molecule comprising a gene which codes for a carrier protein-like polypeptide.
Claim 2
Dependent Claim
13 words
A DNA molecule comprising a gene which codes for a carrier protein-like polypeptide.
Claim 3
Dependent Claim
13 words
A DNA molecule of claim 1, wherein the gene is free of introns.
Claim 4
Dependent Claim
27 words
A DNA molecule of claim 1, wherein the DNA molecule is essentially free of genes which code for any other polypeptide coded for by the human genome.
Claim 5
Dependent Claim
31 words
A DNA molecule of claim 1, wherein the gene codes for a polypeptide having a molecular weight of about 40,000-50,000 daltons or less, if measured in a form accompanied by glycosylation.
Claim 6
Dependent Claim
66 words
A process for obtaining a DNA molecule, comprising preparing cDNA molecules from mRNA found in cells or tissues that produce the carrier protein, determining which of the cDNA molecules hybridize to one or more labelled polynucleotide probes based on the DNA sequence of Figure 4, analyzing the cDNA molecules that hybridized, and obtaining a DNA molecule having a gene which codes for a carrier protein-like polypeptide.
Claim 7
Dependent Claim
66 words
A process for obtaining a DNA molecule, comprising preparing cDNA molecules from mRNA found in cells or tissues that produce the carrier protein, determining which of the cDNA molecules hybridize to one or more labelled polynucleotide probes based on the DNA sequence of Figure 4, analyzing the cDNA molecules that hybridized, and obtaining a DNA molecule having a gene which codes for a carrier protein-like polypeptide.
Claim 8
Dependent Claim
33 words
A DNA molecule obtainable by the process of claim 5, and a DNA molecule which encodes a carrier protein-like polypeptide coded for by a DNA molecule obtainable by the process of claim 5.
Claim 9
Dependent Claim
73 words
A DNA molecule selected from the group consisting of the DNA molecules LCP 0.70, LCP 0.77, LCP 2.3, LCP 2.5, FCP 1.8, and FCP 2.5, DNA molecules which hybridize to any of the DNA molecules LCP 0.70, LCP 0.77, LCP 2.3, LCP 2.5, FCP 1.8 and FCP 2.5 and which code for a carrier protein-like polypeptide, and DNA molecules which code for a polypeptide coded for by any of the foregoing DNA molecules.
Claim 10
Dependent Claim
73 words
A DNA molecule selected from the group consisting of the DNA molecules LCP 0.70, LCP 0.77, LCP 2.3, LCP 2.5, FCP 1.8, and FCP 2.5, DNA molecules which hybridize to any of the DNA molecules LCP 0.70, LCP 0.77, LCP 2.3, LCP 2.5, FCP 1.8 and FCP 2.5 and which code for a carrier protein-like polypeptide, and DNA molecules which code for a polypeptide coded for by any of the foregoing DNA molecules.
Claim 11
Dependent Claim
23 words
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids -1 to 290 of Figure 4.
Claim 12
Dependent Claim
23 words
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids -1 to 290 of Figure 4.
Claim 13
Dependent Claim
23 words
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 of 290 of Figure 4.
Claim 14
Dependent Claim
23 words
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 of 290 of Figure 4.
Claim 15
Dependent Claim
32 words
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 to 290 of Figure 4 and having a methionine residue preceding amino acid 27.
Claim 16
Dependent Claim
32 words
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 to 290 of Figure 4 and having a methionine residue preceding amino acid 27.
Claim 17
Dependent Claim
28 words
A recombinant DNA molecule comprising a DNA molecule according to any one of claims 1 to 4 and 6 to 10 operatively linked to an expression control sequence.
Claim 18
Dependent Claim
14 words
A host transformed with at least one recombinant DNA molecule according to claim 11.
Claim 19
Dependent Claim
20 words
A method for producing a carrier protein-like polypeptide, comprising culturing a host according to claim 12 to make said polypeptide.
Claim 20
Dependent Claim
11 words
A vector comprising a recombinant DNA molecule according to claim 11.
Claim 21
Dependent Claim
11 words
A host transformed with at least one vector of claim 14.
Claim 22
Dependent Claim
24 words
A method for producing a carrier protein-like polypeptide, comprising culturing a host transformed by a vector according to claim 14 to make said polypeptide.
Claim 23
Dependent Claim
17 words
An essentially pure carrier protein-like polypeptide other than a carrier protein subunit capable of binding somatomedin-like polypeptides.
Claim 24
Dependent Claim
17 words
An essentially pure carrier protein-like polypeptide other than a carrier protein subunit capable of binding somatomedin-like polypeptides.
Claim 25
Dependent Claim
12 words
A carrier protein-like polypeptide lacking the natural glycosylation of the carrier protein.
Claim 26
Dependent Claim
12 words
A carrier protein-like polypeptide lacking the natural glycosylation of the carrier protein.
Claim 27
Dependent Claim
15 words
A polypeptide of claim 18 being essentially free of substances naturally present in human serum.
Claim 28
Dependent Claim
28 words
An essentially pure polypeptide having the sequence of amino acids 27 to 290 of Figure 4 and such a polypeptide having a methinonine residue preceding amino acid 27.
Claim 29
Dependent Claim
28 words
An essentially pure polypeptide having the sequence of amino acids 27 to 290 of Figure 4 and such a polypeptide having a methinonine residue preceding amino acid 27.
Claim 30
Dependent Claim
26 words
The polypeptide of claim 20 wherein one or more amino acid residues have been added, deleted or substituted and the polypeptide constitutes a carrier protein-like polypeptide.
Claim 31
Dependent Claim
36 words
A polypeptide selected from the group consisting of a polypeptide having the sequence of amino acids -1 to 290 of Figure 4, and polypeptides having a portion of that sequence and constituting a carrier protein-like polypeptide.
Claim 32
Dependent Claim
36 words
A polypeptide selected from the group consisting of a polypeptide having the sequence of amino acids -1 to 290 of Figure 4, and polypeptides having a portion of that sequence and constituting a carrier protein-like polypeptide.
Claim 33
Dependent Claim
99 words
A therapeutic composition comprising an effective amount for inhibiting the effect of somatomedin-C in acromegaly, for inhibiting the growth of retinal blood vessels and fibrous tissues in diabetic retinopathy, for inhibiting growth of tall children, for inhibiting the growth of keloid scars, for inhibiting the growth of tissue in the orbit of the eyes in malignant exophthalmos or for stimulating the healing of human or animal wounds, of at least one polypeptide selected from the group consisting of a polypeptide according to any one of claims 17 to 22, or a pharmacologically-acceptable salt thereof, and a pharmacologically acceptable carrier.
Claim 34
Dependent Claim
81 words
A method for inhibiting the growth of somatomedin-dependent cancers, for inhibiting the effect of somatomedin-C in acromegaly, for inhibiting the growth of retinal blood vessels and fibrous tissues in diabetic retinopathy, for inhibiting growth of tall children, for inhibiting the growth of keloid scars, for inhibiting the growth of tissue in the orbit of the eyes in malignant exophthalmos or for stimulating the healing of human or animal wounds, comprising administering an effective amount of a composition according to claim 23
Claim 35
Dependent Claim
32 words
A composition comprising at least one polypeptide selected from the group consisting of a polypeptide according to any one of claims 17 to 22 substantially complexed with at least one somatomedin-like polypeptide.
Claim 36
Dependent Claim
47 words
A therapeutic composition comprising an effective amount for treating osteoporosis in humans, for stimulating the growth of bone, for stimulating animal growth, for stimulating the healing of human and animal wounds, for stimulating the growth of patients with growth hormone deficiency, of a composition of claim 25.
Claim 37
Dependent Claim
48 words
A method for treating osteoporosis in humans, for stimulating the growth of bone, for stimulating animal growth, for stimulating the healing of human and animal wounds, for stimulating the growth of patients with growth hormone deficiency, comprising administering an effective amount of a composition according to claim 26.
Claim 38
Dependent Claim
10 words
Monoclonal and polyclonal antibodies against a polypeptide of claim 17.
Claim 39
Dependent Claim
25 words
A method for measuring the level of free somatomedins in human fluids comprising separating somatomedins complexed with a polypeptide of claim 17 from unbound somatomedins.
References Cited (Backward Citations)
No citation records indexed.
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