EP0374044B1
Expired / Lapsed
EP
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TGF - beta 1 / beta 2 : a novel chimeric transforming growth factor-beta.
Assignee: Oncogen LP
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Filing: 1989-12-13
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Granted: 1994-09-14
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Expires: 2009-12-13
Abstract
Abstract The present invention relates to a chimeric transforming growth factor- beta 1/ beta 2 comprising the amino acid sequence substantially as depicted in FIG. 1 from about amino acid number 279 to about amino acid residue number 390, and to the production by recombinant DNA methods.
Inventors
- 👤 Anthony F. Purchio
- 👤 Linda Madisen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 15:21
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/495; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
31 words
A method for preparing a chimeric transforming growth factor-β1/β2 comprising preparing the amino acid sequence as depicted in Fig. 1 from amino acid number 279 to amino acid residue number 390.
Claim 2
Independent Claim
35 words
A method for preparing a DNA molecule encoding chimeric transforming growth factor-β1/β2 comprising isolating the amino acid sequence as depicted in Fig. 1 from amino acid residue number 279 to amino acid residue number 390.
Claim 3
Independent Claim
35 words
A method for preparing a DNA molecule encoding chimeric transforming growth factor-β1/β2 comprising isolating the amino acid sequence as depicted in Fig. 1 from amino acid residue number 1 to amino acid residue number 390.
Claim 4
Independent Claim
44 words
A method for preparing a transformed mammalian cell wherein a mammalian cell is transformed with a DNA molecule coding for chimeric transforming growth factor-β1/β2 comprising the amino acid sequence as depicted in Fig. 1 from amino acid number 279 to amino acid number 390.
Claim 5
Independent Claim
44 words
A method for preparing a transformed mammalian cell wherein a mammalian cell is transformed with a DNA molecule coding for chimeric transforming growth factor-β1/β2 comprising the amino acid sequence as depicted in Fig. 1 from amino acid number 1 to amino acid number 390.
Claim 6
Independent Claim
73 words
A method for preparing a transformed mammalian cell wherein a mammalian cell is transformed with a DNA molecule coding for chimeric transforming growth factor-β1/β2 comprising the amino acid sequence as depicted in Fig. 1 from amino acid number 279 to amino acid number 390, wherein the coding region of said DNA molecule is under the control of a nucleotide sequence that regulates gene expression so that the cell produces chimeric transforming growth factor-β1/β2.
Claim 7
Independent Claim
74 words
A method for preparing a transformed mammalian cell wherein a mammalian cell is transformed with a DNA molecule coding for chimeric transforming growth factor-β1/β2 comprising the amino acid sequence as depicted in Fig. 1 from amino acid number 1 to amino acid number 390, wherein the coding region of said DNA molecule is under the control of a nucleotide sequence that regulates gene expression so that the cell produces chimeric transforming growth factor-β1/β2 .
Claim 8
Independent Claim
15 words
The method according to Claims 6 and 7 which is a Chinese Hamster Ovary cell.
Claim 9
Dependent Claim
21 words
The method according to Claim 6 or 7 in which the nucleotide sequence that regulates gene expression comprises an SV40 promoter.
Claim 10
Dependent Claim
28 words
The method according to Claim 6 or 7 in which the nucleotide sequence that regulates gene expression comprises a promoter and a coding sequence for a selectable marker.
Claim 11
Dependent Claim
14 words
The method according to Claim 10 in which the selectable marker is dihydrofolate reductase.
Claim 12
Independent Claim
95 words
A method for producing chimeric transforming growth factor-β1/β2 comprising: (a) culturing a mammalian host cell transformed with a DNA molecule coding for chimeric transforming growth factor-β1/β2 comprising the amino acid sequence as depicted in FIG. 1 from amino acid number 279 to amino acid number 390, wherein the coding region of said DNA molecule is under the control of a nucleotide sequence that regulates gene expression so that a peptide or protein having chimeric transforming growth factor-β1/β2 activity is produced by the host cell; and (b) recovering the chimeric transforming growth factor-β1/β2 from the culture.
Claim 13
Independent Claim
95 words
A method for producing chimeric transforming growth factor-β1/β2 comprising: (a) culturing a mammalian host cell transformed with a DNA molecule coding for chimeric transforming growth factor-β1/β2, comprising the amino acid sequence as depicted in FIG. 1 from amino acid number 1 to amino acid number 390, wherein the coding region of said DNA molecule is under the control of a nucleotide sequence that regulates gene expression so that a peptide or protein having chimeric transforming growth factor-β1/β2 activity is produced by the host cell; and (b) recovering the chimeric transforming growth factor-β1/β2 from the culture.
Claim 14
Dependent Claim
19 words
The method according to Claim 12 or 13 in which the host cell is a Chinese Hamster Ovary Cell.
Claim 15
Dependent Claim
21 words
The method according to Claim 12 or 13 in which the nucleotide sequence which regulates gene expression comprises an SV40 promoter.
Claim 16
Dependent Claim
51 words
The method according to Claim 12 or 13 in which the nucleotide sequence that regulates gene expression comprises a promoter and a coding sequence for a selectable marker for which the host cell is deficient, so that the host cell containing the chimeric transforming growth factor-β1/β2 coding sequence can be identified.
Claim 17
Dependent Claim
14 words
The method according to Claim 16 in which the selectable marker is dihydrofolate reductase.
Claim 18
Dependent Claim
37 words
The method according to Claim 17 further comprising exposing the host cell to methotrexate so that resistant colonies are selected which contain amplified levels of the coding sequence for dihydrofolate reductase and the chimeric transforming growth factor-β1/β2.
Claim 19
Independent Claim
36 words
A method for producing chimeric transforming growth factor-β1/β2, comprising: (a) culturing transfectant CHO-5β41,2.5 CL5, deposited with the American Type Culture Collection and having accession No. CRL 9959; (b) recovering chimeric transforming growth factor-β1/β2 from the culture.
Claim 20
Dependent Claim
17 words
The method according to Claim 19 in which the transfectant is cultured in the presence of methotrexate.
References Cited (Backward Citations)
No citation records indexed.
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