EP0374044A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
TGF - beta 1 / beta 2 : a novel chimeric transforming growth factor-beta.
Assignee: Oncogen LP
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Filing: 1989-12-13
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Granted: —
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Expires: 2009-12-13
Abstract
Abstract The present invention relates to a chimeric transforming growth factor-β1/β2 comprising the amino acid sequence substantially as depicted in FIG. 1 from about amino acid number 279 to about amino acid residue number 390, and to the production by recombinant DNA methods.
Inventors
- 👤 Anthony F. Purchio
- 👤 Linda Madisen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 16:15
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/495; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
29 words
A chimeric transforming growth factor-β1/β2 comprising the amino acid sequence substantially as depicted in FIG. 1 from about amino acid number 279 to about amino acid residue number 390.
Claim 2
Dependent Claim
29 words
A chimeric transforming growth factor-β1/β2 comprising the amino acid sequence substantially as depicted in FIG. 1 from about amino acid number 279 to about amino acid residue number 390.
Claim 3
Dependent Claim
31 words
A nucleotide sequence encoding chimeric transforming growth factor-β1/β2 comprising the nucleotide coding sequence substantially as depicted in FIG. 1 from about nucleotide residue number 836 to about nucleotide residue number 1170.
Claim 4
Dependent Claim
31 words
A nucleotide sequence encoding chimeric transforming growth factor-β1/β2 comprising the nucleotide coding sequence substantially as depicted in FIG. 1 from about nucleotide residue number 836 to about nucleotide residue number 1170.
Claim 5
Dependent Claim
31 words
A nucleotide sequence encoding chimeric transforming growth factor-β1/β2 comprising the nucleotide coding sequence substantially as depicted in FIG. 1 from about nucleotide residue number 1 to about nucleotide residue number 1170.
Claim 6
Dependent Claim
31 words
A nucleotide sequence encoding chimeric transforming growth factor-β1/β2 comprising the nucleotide coding sequence substantially as depicted in FIG. 1 from about nucleotide residue number 1 to about nucleotide residue number 1170.
Claim 7
Dependent Claim
28 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 836 to about nucleotide number 1170.
Claim 8
Dependent Claim
28 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 836 to about nucleotide number 1170.
Claim 9
Dependent Claim
28 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 1 to about nucleotide number 1170.
Claim 10
Dependent Claim
28 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 1 to about nucleotide number 1170.
Claim 11
Dependent Claim
49 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 836 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that the cell produces chimeric transforming growth factor-β1/β2.
Claim 12
Dependent Claim
49 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 836 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that the cell produces chimeric transforming growth factor-β1/β2.
Claim 13
Dependent Claim
49 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 1 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that the cell produces chimeric transforming growth factor-β1/β2.
Claim 14
Dependent Claim
49 words
A cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2 substantially as depicted in FIG. 1 from about nucleotide number 1 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that the cell produces chimeric transforming growth factor-β1/β2.
Claim 15
Dependent Claim
15 words
The cell according to Claim 6 or 7 which comprises a Chinese Hamster Ovary cell.
Claim 16
Dependent Claim
22 words
The cell according to Claim 6 or 7 in which the second nucleotide sequence that regulates gene expression comprises an SV40 promoter.
Claim 17
Dependent Claim
24 words
The cell according to Claim 6 or 7 in which the second sequence comprises a promoter and a coding sequence for a selectable marker.
Claim 18
Dependent Claim
14 words
The cell according to Claim 10 in which the selectable marker comprises dihydrofolate reductase.
Claim 19
Dependent Claim
18 words
A cell line comprises CHO-5β41,2.5 CL5 as deposited with the American Type Culture Collection, having accession No. .
Claim 20
Dependent Claim
18 words
A cell line comprises CHO-5β41,2.5 CL5 as deposited with the American Type Culture Collection, having accession No. .
Claim 21
Independent Claim
81 words
A method for producing chimeric transforming growth factor-β1/β2 comprising: (a) culturing a host cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2, substantially as depicted in FIG. 1 from about nucleotide number 836 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that a peptide or protein having chimeric transforming growth factor-β1/β2 activity is produced by the host cell; and (b) recovering the chimeric transforming growth factor-β1/β2 from the culture.
Claim 22
Independent Claim
81 words
A method for producing chimeric transforming growth factor-β1/β2 comprising: (a) culturing a host cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1/β2, substantially as depicted in FIG. 1 from about nucleotide number 836 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that a peptide or protein having chimeric transforming growth factor-β1/β2 activity is produced by the host cell; and (b) recovering the chimeric transforming growth factor-β1/β2 from the culture.
Claim 23
Independent Claim
81 words
A method for producing chimeric transforming growth factor-β1/β2 comprising: (a) culturing a host cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1,β2, substantially as depicted in FIG. 1 from about nucleotide number 1 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that a peptide or protein having chimeric transforming growth factor-β1/β2 activity is produced by the host cell; and (b) recovery the chimeric transforming growth factor-β1/β2 from the culture.
Claim 24
Independent Claim
81 words
A method for producing chimeric transforming growth factor-β1/β2 comprising: (a) culturing a host cell containing a nucleotide coding sequence for chimeric transforming growth factor-β1,β2, substantially as depicted in FIG. 1 from about nucleotide number 1 to about nucleotide number 1170, under the control of a second nucleotide sequence that regulates gene expression so that a peptide or protein having chimeric transforming growth factor-β1/β2 activity is produced by the host cell; and (b) recovery the chimeric transforming growth factor-β1/β2 from the culture.
Claim 25
Dependent Claim
19 words
The method according to Claim 13 or 14 in which the host cell comprises a Chinese Hamster Ovary Cell.
Claim 26
Dependent Claim
22 words
The method according to Claim 13 or 14 in which the second nucleotide sequence which regulates gene expression comprises an SV40 promoter.
Claim 27
Dependent Claim
48 words
The method according to Claim 13 or 14 in which the second nucleotide sequence comprises a promoter and a coding sequence for a selectable marker for which the host cell is deficient, so that the host cell containing the chimeric transforming growth factor-β1/β2 coding sequence can be identified.
Claim 28
Dependent Claim
14 words
The method according to Claim 17 in which the selectable marker comprises dihydrofolate reductase.
Claim 29
Dependent Claim
37 words
The method according to Claim 18 further comprising exposing the host cell to methotrexate so that resistant colonies are selected which contain amplified levels of the coding sequence for dihydrofolate reductase and the chimeric transforming growth factor-β1/β2.
Claim 30
Independent Claim
37 words
A method for producing chimeric transforming growth factor-β1/β2, comprising: (a) culturing transfectant CHO-5β41,2.5 CL5, deposited with the American Type Culture Collection and having accession No. CRL 9959 ; (b) recovering chimeric transforming growth factor-β1/β2 from the culture.
Claim 31
Independent Claim
37 words
A method for producing chimeric transforming growth factor-β1/β2, comprising: (a) culturing transfectant CHO-5β41,2.5 CL5, deposited with the American Type Culture Collection and having accession No. CRL 9959 ; (b) recovering chimeric transforming growth factor-β1/β2 from the culture.
Claim 32
Dependent Claim
17 words
The method according to Claim 20 in which the transfectant is cultured in the presence of methotrexate.
References Cited (Backward Citations)
No citation records indexed.
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