EP0355989A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
DNA and RNA probes, methods and kits for the detection of salmonella
Assignee: Shimadzu Corp
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Filing: 1989-07-19
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Granted: —
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Expires: 2009-07-19
Abstract
Abstract A probe for detecting Salmonella comprises: a labeled substance, and a DNA or RNA fragment which hybridizes with a base sequence having the following formula ( I ), or ( II ) being complementary to the formula ( I ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ ( II ) Also described are methods and kits for detecting Salmonella by using said probe. The probe reduces a time required for detecting Salmonella .
Inventors
- 👤 Hiroyuki Jikuya
- 👤 Tetuo Ohashi
- 👤 Jun Takano
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 02:13
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/879; Y10S436/00; Y10S436/811
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
46 words
A DNA probe for detecting Salmonella which comprises: a label substance, and a DNA fragment which hybridizes with a base sequence, which base sequence has the following formula ( I ), or the complementary formula ( II ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ ( II ).
Claim 2
Dependent Claim
46 words
A DNA probe for detecting Salmonella which comprises: a label substance, and a DNA fragment which hybridizes with a base sequence, which base sequence has the following formula ( I ), or the complementary formula ( II ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ ( II ).
Claim 3
Dependent Claim
46 words
A RNA probe for detecting Salmonella which comprises: a label substance, and a RNA fragment which hybridizes with a base sequence which base sequence has the following formula ( I ), or the complementary formula ( II ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ ( II ).
Claim 4
Dependent Claim
46 words
A RNA probe for detecting Salmonella which comprises: a label substance, and a RNA fragment which hybridizes with a base sequence which base sequence has the following formula ( I ), or the complementary formula ( II ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ ( II ).
Claim 5
Dependent Claim
41 words
A DNA probe for detecting Salmonella which comprises: a label substance; and a DNA fragment which comprises part or all of a base sequence having the following formula ( I ) or ( II ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ (II).
Claim 6
Dependent Claim
41 words
A DNA probe for detecting Salmonella which comprises: a label substance; and a DNA fragment which comprises part or all of a base sequence having the following formula ( I ) or ( II ) 5′-GCTCAGACGTATGGCGGTA-3′ ( I ) 3′-CGAGTCTGCATACCGCCAT-5′ (II).
Claim 7
Dependent Claim
43 words
A RNA probe for detecting Salmonella which comprises: a label substance; and a RNA fragment which comprises part or all of a base sequence having the following formula ( I′ ) or ( II′ ) 5′-GCUCAGACGUAUGGCGGUA-3′ ( I′ ) 3′-CGAGUCUGCAUACCGCCAU-5′ ( II′ ).
Claim 8
Dependent Claim
43 words
A RNA probe for detecting Salmonella which comprises: a label substance; and a RNA fragment which comprises part or all of a base sequence having the following formula ( I′ ) or ( II′ ) 5′-GCUCAGACGUAUGGCGGUA-3′ ( I′ ) 3′-CGAGUCUGCAUACCGCCAU-5′ ( II′ ).
Claim 9
Independent Claim
10 words
A method for detecting Salmonella in a sample, which comprises:
Claim 10
Independent Claim
10 words
A method for detecting Salmonella in a sample, which comprises:
Claim 11
Dependent Claim
35 words
contacting the probe according to any one of claims 1-4 with a sample under conditions in which the probe can hybridize with Salmonella DNA, and detecting the existence of a DNA-DNA or DNA-RNA hybridization complex.
Claim 12
Dependent Claim
18 words
A kit for the detection of Salmonella which comprises a probe according to any one of claims 1-4.
References Cited (Backward Citations)
No citation records indexed.
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